Validation of an ELISA method for the serological diagnosis of canine brucellosis due to Brucella canis

Oliveira, Maria Z.D.; Vale, Vera Lúcia Costa; Keid, Lara Borges; Freire, Songelí Menezes; Meyer, Roberto; Portela, Ricardo Wagner; Barrouin‐Melo, Stella Maria

doi:10.1016/j.rvsc.2010.07.004

Cited by 30 publications

(27 citation statements)

References 27 publications

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“…However, the AGID is not used by many laboratories today because of its low sensitivity and because it requires trained personnel and special media [8]. Enzyme-linked immunosorbent assays (ELISA) have been developed [58] and a lateral flow immune-chromatographic assay (LFIA) has recently been described [59]. A combination of different serological tests is often recommended.…”

Section: Introductionmentioning

confidence: 99%

The first case of Brucella canis in Sweden: background, case report and recommendations from a northern European perspective

et al. 2012

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Infection with Brucella canis has been diagnosed in Sweden for the first time. It was diagnosed in a three-year-old breeding bitch with reproductive disturbances. Fifteen in-contact dogs were tested repeatedly and all of them were negative for B. canis . The source of infection could not be defined. The present article describes the case and the measures undertaken and gives a short review over B. canis . Recommendations on how to avoid the infection in non-endemic countries are given.

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Section: Introductionmentioning

confidence: 99%

The first case of Brucella canis in Sweden: background, case report and recommendations from a northern European perspective

et al. 2012

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“…The cut-off values were generated using the point of maximum of combined specificity and sensitivity for each ROC curve (Ren et al, 2018). The validation parameters were calculated as described by de Oliveira et al (2011). Repeatability was calculated for the negative and positive control serum sample pools tested 20 times at the same moment and by the same operator, using the formula RP = (1 -CV) 9 100, where RP stands for repeatability and CV for coefficient of variation.…”

Section: Discussionmentioning

confidence: 99%

A panel of recombinant proteins for the serodiagnosis of caseous lymphadenitis in goats and sheep

Barral¹,

Mariutti

Arni

et al. 2019

Microbial Biotechnology

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Summary Caseous lymphadenitis (CLA) is a small ruminant disease characterized by the development of granulomatous lesions in superficial and internal lymph nodes, as well as in some organs, and causes significant economic losses worldwide. The aetiological agent of CLA is the bacterium Corynebacterium pseudotuberculosis; however, the commercially available diagnostic tools present problems with regard to specificity, which can lead to false‐negative results. This study aimed to develop an indirect enzyme‐linked immunosorbent assay (ELISA) for the detection of specific immunoglobulins in goats and sheep using recombinant C. pseudotuberculosis PLD, CP40, PknG, DtxR and Grx proteins. For validation of the ELISAs, 130 goat serum samples and 160 sheep serum samples were used. The best ELISA for goats was developed using a combination of PLD and CP40 as antigens at a 1:1 ratio, which presented 96.9% sensitivity and 98.4% specificity. The most effective ELISA for sheep presented 91% sensitivity and 98.7% specificity when recombinant PLD alone was used as the antigen. These ELISAs can be used as highly accurate tools in epidemiological surveys and for the serodiagnosis of C. pseudotuberculosis infection in goats and sheep.

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“…Brucella contributes to abortions, infertility, placenta retention, still birth or birth of weak off spring, and poor reproductive performance of animals, which results in huge economic losses for livestock farmers [6]. Currently, there is no effective method to prevent such pathophoresis, contributing to the increased attention on the rapid detection of Brucella.…”

Section: Introductionmentioning

confidence: 99%

A quantum dot fluorescent microsphere based immunochromatographic strip for detection of brucellosis

Kong

Wang

et al. 2020

Preprint

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Abstract Background: Brucellosis is a serious zoonosis disease that frequently causes significant economic loss in animal husbandry and threatens human health. Therefore, we established a rapid, accurate, simple and sensitive fluorescent immunochromatographic test strip (ICTS) based on quantum dots to detect serum antibodies of brucellosis.Result: The test strips were successfully prepared by quantum dot fluorescent microspheres (QDFM) as tracers, which were covalently coupled to an outer membrane protein of Brucella, OMP22. The outer membrane protein OMP28 and monoclonal antibodies of OMP22 against Brucella were separately dispensed onto a nitrocellulose membrane as test and quality control lines, respectively. Antibodies specific for brucellosis was detected qualitatively using the ratio of the fluorescence signals of the test and control lines (HT/HC). The threshold of assay was determined as a HT/HC value of 0.0492. The repeatability was excellent with an overall average CV of 8.78%. Under optimum conditions, the limit of detection was 1.05 ng/mL (1:512 dilution). With regard to the detection of brucellosis in 150 clinical samples, the total coincidence rate of ICTS and Rose Bengal plate test (RBPT) was 97.3%, the coincidence rate of positive samples was 98.8%, the coincidence rate of negative samples was 95.3%, and no cross reaction with the sera of other related diseaseswas observed.Conclusion: In our present study, the QDFM has promising application for on-site screening of brucellosis owing to its high detection speed, high sensitivity high specificity and low cost.

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Validation of an ELISA method for the serological diagnosis of canine brucellosis due to Brucella canis

Cited by 30 publications

References 27 publications

The first case of Brucella canis in Sweden: background, case report and recommendations from a northern European perspective

The first case of Brucella canis in Sweden: background, case report and recommendations from a northern European perspective

A panel of recombinant proteins for the serodiagnosis of caseous lymphadenitis in goats and sheep

A quantum dot fluorescent microsphere based immunochromatographic strip for detection of brucellosis

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