Validation of an automated immune turbidimetric assay for serum gelsolin and its possible clinical utility in sepsis

Horváth‐Szalai, Zoltán; Kabos, Peter; Szirmay, Balázs; Lakatos, Ágnes; Christensen, Per Hjort; Huber, Tamás; Bugyi, Beáta; Mühl, Diána; Ludány, Andrea; Miseta, Attila; Kovaćs, Gábor L.; Kőszegi, Tamás

doi:10.1002/jcla.22321

Cited by 6 publications

(5 citation statements)

References 17 publications

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“…Nevertheless, its clinical applicability is limited by the lack of rapid laboratory methods for determination of actin and gelsolin in patients’ samples [ 102 ]. In response to this issue, novel turbidimetric measurements of gelsolin have been developed, offering a rapid and accurate quantitation of gelsolin in human serum samples; nevertheless, more accurate analyses are needed to introduce this analytic method into clinical practice [ 104 ]. Another proposition from this research team involves the simultaneous determination of plasma gelsolin and Gc-globulin.…”

Section: Alterations In Plasma Gelsolin Concentrations In Differenmentioning

confidence: 99%

“…Notably, none of these methods are routinely applicable in clinical settings due to the high cost of reagents, equipment, significant personnel involvement, and the long duration of the analysis. Considering this, novel reports about the development of an automated serum gelsolin immune turbidimetric assay for possible clinical use are particularly interesting [ 104 ].…”

Section: Alterations In Plasma Gelsolin Concentrations In Differenmentioning

confidence: 99%

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Plasma Gelsolin: Indicator of Inflammation and Its Potential as a Diagnostic Tool and Therapeutic Target

Piktel

Levental

Durnaś

et al. 2018

IJMS

102

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Gelsolin, an actin-depolymerizing protein expressed both in extracellular fluids and in the cytoplasm of a majority of human cells, has been recently implicated in a variety of both physiological and pathological processes. Its extracellular isoform, called plasma gelsolin (pGSN), is present in blood, cerebrospinal fluid, milk, urine, and other extracellular fluids. This isoform has been recognized as a potential biomarker of inflammatory-associated medical conditions, allowing for the prediction of illness severity, recovery, efficacy of treatment, and clinical outcome. A compelling number of animal studies also demonstrate a broad spectrum of beneficial effects mediated by gelsolin, suggesting therapeutic utility for extracellular recombinant gelsolin. In the review, we summarize the current data related to the potential of pGSN as an inflammatory predictor and therapeutic target, discuss gelsolin-mediated mechanisms of action, and highlight recent progress in the clinical use of pGSN.

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Section: Alterations In Plasma Gelsolin Concentrations In Differenmentioning

confidence: 99%

Section: Alterations In Plasma Gelsolin Concentrations In Differenmentioning

confidence: 99%

Plasma Gelsolin: Indicator of Inflammation and Its Potential as a Diagnostic Tool and Therapeutic Target

Piktel

Levental

Durnaś

et al. 2018

IJMS

102

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“…They found a significant decrease in pGSN level with increased severity of sepsis ( P = .009). [ 18 ] Similarly, Horváth-Szalai et al [ 20 ] found that pGSN levels were lower in patients with severe sepsis as compared to those with sepsis [16.74 (8.21–34.57) and 26.31 (16.89–28.25) μg/mL, respectively].…”

Section: Discussionmentioning

confidence: 99%

Late onset neonatal sepsis: Can plasma gelsolin be a promising diagnostic marker?

Mokhtar,

Sherief,

Kamal

et al. 2024

Medicine

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Plasma gelsolin (pGSN) correlates with clinical improvement in septic patients. We aimed to investigate pGSN levels as a diagnostic and prognostic marker of neonatal late-onset-sepsis (LOS). A case-control study was done on 184 neonates (92 with LOS and 92 controls). All participants were subjected to detailed history taking, full clinical evaluation, sepsis workup, and pGSN enzyme-linked immunosorbent-assay measurement. We detected significantly lower pGSN level among cases compared to controls (90.63 ± 20.64 vs 451.83 ± 209.59). It was significantly related to the severity of sepsis and mortality, with significantly lower values among cases with septic shock and multiorgan failure and non-survivors. Follow-up pGSN significantly increased after sepsis improvement in survivors compared to admission values. pGSN might be a reliable diagnostic and prognostic marker for LOS.

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“…Serum total protein (TP), albumin, creatinine, hs-CRP, PCT, gelsolin (GSN), Gcglobulin, blood cell counts were determined by routine automated laboratory methods and/or our published automated tests [18,20] in our accredited Department of Laboratory Medicine, Hungary (NAH 9-0008/2021). Urinary parameters (u-TP, u-albumin, u-orosomucoid, u-Cystatin C) were obtained by our previously published automated techniques [14,19] and u-Gc by the present ELISA method.…”

Section: Clinical Data and Laboratory Testingmentioning

confidence: 99%

“…However, many other markers have been tested for the diagnosis and follow-up of sepsis such as presepsin, calprotectin, proinflammatory interleukins, monocyte chemoattractant protein-1, pentraxin-3, mid-regional fragment of pro-adrenomedullin and many others [13]. Trying to establish novel sepsis markers with a potential predictive value regarding organ dysfunctions as well, during the past few years, our research group has found non-commercially available complementary laboratory tests [10,[14][15][16][17][18][19][20][21][22] and adapted most of them to automated laboratory instrumentation including serum gelsolin (GSN), Gc-globulin (Gc), presepsin/GSN ratio, and urinary markers such as orosomucoid (u-ORM), Cystatin C (u-CysC) and neutrophil gelatinase associated lipocalin (u-NGAL).…”

Section: Introductionmentioning

confidence: 99%

Measurement of Urinary Gc-Globulin by a Fluorescence ELISA Technique: Method Validation and Clinical Evaluation in Septic Patients—A Pilot Study

Kőszegi,

Horváth-Szalai,

Ragán

et al. 2023

Molecules

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A major complication of sepsis is the development of acute kidney injury (AKI). In case of acute tubular damage, Gc-globulin, a known serum sepsis marker is increasingly filtrated into the urine therefore, urinary Gc-globulin (u-Gc) levels may predict septic AKI. We developed and validated a competitive fluorescence ELISA method for u-Gc measurement. Serum and urine samples from septic patients were collected in three consecutive days (T1, T2, T3) and data were compared to controls. Intra- and interassay imprecisions were CV < 14% and CV < 20%, respectively, with a recovery close to 100%. Controls and septic patients differed (p < 0.001) in their u-Gc/u-creatinine levels at admission (T1, median: 0.51 vs. 79.1 µg/mmol), T2 (median: 0.51 vs. 57.8 µg/mmol) and T3 (median: 0.51 vs. 55.6 µg/mmol). Septic patients with AKI expressed higher u-Gc/u-creatinine values than those without AKI at T1 (median: 23.6 vs. 136.5 µg/mmol, p < 0.01) and T3 (median: 34.4 vs. 75.8 µg/mmol, p < 0.05). AKI-2 stage patients exhibited more increased u-Gc/u-creatinine levels at T1 (median: 207.1 vs. 53.3 µg/mmol, p < 0.05) than AKI-1 stage individuals. Moderate correlations (p < 0.001) were observed between u-Gc/u-creatinine and se-urea, se-creatinine, se-hsCRP, WBC, u-total protein, u-albumin, u-orosomucoid/u-creatinine, and u-Cystatin C/u-creatinine levels. U-Gc testing may have a predictive value for AKI in septic patients.

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Validation of an automated immune turbidimetric assay for serum gelsolin and its possible clinical utility in sepsis

Abstract: Based on the results, our immune turbidimetric measurement offers a rapid and accurate quantitation of gelsolin in human serum samples. Serum gelsolin seems a promising additional diagnostic marker of sepsis which has to be further investigated.

Cited by 6 publications

References 17 publications

Plasma Gelsolin: Indicator of Inflammation and Its Potential as a Diagnostic Tool and Therapeutic Target

Plasma Gelsolin: Indicator of Inflammation and Its Potential as a Diagnostic Tool and Therapeutic Target

Late onset neonatal sepsis: Can plasma gelsolin be a promising diagnostic marker?

Measurement of Urinary Gc-Globulin by a Fluorescence ELISA Technique: Method Validation and Clinical Evaluation in Septic Patients—A Pilot Study

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