Validation of a simple, sensitive method for the determination of β-estradiol in bovine urine using gas-chromatography negative-ion chemical ionization mass spectrometry

Abstract

A new method is presented for the analysis of 17[small beta]-estradiol in bovine urine. After deconjugation, the sample is cleaned up using an OASIS[trade mark sign] HLB disposable cartridge and extracted into 1-chlorobutane. The hormone is derivatized using pentafluorobenzoyl chloride. The derivatized estradiol is quantitated using gas-chromatography negative-ion chemical ionization mass spectrometry. Calibrations, obtained using spiked blank urine, are linear in the range of 100-1000 pg mL(-1) with CC[small alpha] approximately 170 pg mL(-1) and CC[small beta] of 287 pg mL(-1). Recoveries are in the range of 80 to 130%. The method is rugged, rapid and sensitive when compared to other hormone methods.

Picogram-level quantification of some growth hormones in bovine urine using mixed-solvent bubble-in-drop single drop micro-extraction

“…The set-up employs C4, C8 and C18 pre-column cartridges in the analysis of alkylphenolic compounds and steroid sex hormones in sediments at ng mL -1 levels. Solid phase extraction using commercially available phases and molecularly imprinted polymers have been used for the analysis of various hormones in bovine urine with LOD values of 0.17 ng mL -1 [17,18]. Additionally, solid phase extraction (SPE) with HPLC-UV techniques have been used to determine levels of steroid hormones in water, with LOD values in the 0.07-0.61 ng g -1 range for five analytes [10].…”

Multiresidue Determination of the Anabolic-Agent Residues Steroids, Stilbenes, and Resorcylic Acid Lactones in Bovine Urine by GC-MS/MS with Microwave-Assisted Derivatization

“…Gas or liquid chromatography–tandem mass spectrometry (GC-MS or LC-MS) is often employed for the identification and quantification of these substances. Brief descriptions of the works available in the literature describing the analysis of anabolic agents in bovine urine are shown in Table . ,− …”

A screening and determinative method for the analysis of natural and synthetic steroids, stilbenes and resorcyclic acid lactones in bovine urine

“…There are many previously published scientific papers describing the analysis of the compounds of interest in bovine urine using LC or GC separations, but they generally focus on one class of compounds. There are very few examples of methods using both GC and LC .…”

“…All the compounds in this method can be classed as growth promoters, estrogen mimickers, or endocrine disruptors due to their estrogenic activity and are banned for use in the EU. [6] There are many previously published scientific papers describing the analysis of the compounds of interest in bovine urine using LC [7,8] or GC [9][10][11] separations, but they generally focus on one class of compounds. There are very few examples of methods using both GC and LC.…”