Validation of a simple and economic HPLC-UV method for the simultaneous determination of vancomycin, meropenem, piperacillin and tazobactam in plasma samples

Milla, Paola; Ferrari, Fiorenza; Muntoni, Elisabetta; Sartori, Marco; Ronco, Claudio; Berlier, Gloria

doi:10.1016/j.jchromb.2020.122151

Cited by 38 publications

(33 citation statements)

References 22 publications

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“…Several HPLC and LC-MS/MS methods have been developed for quantification of antibiotics in plasma [ 223 , 224 , 225 , 226 , 227 , 228 , 229 , 230 ]. HPLC-extraction methods could request more than 100 µL of plasma [ 225 ], these volumes are often not available in preterm neonates. One limit of UV methods coupled with HPLC is the possibility of interfering compounds and long sample-preparation procedures.…”

Section: Therapeutic Drug Monitoring (Tdm) Of Antibiotics In Pretermsmentioning

confidence: 99%

Use of Antibiotics in Preterm Newborns

et al. 2022

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Due to complex maturational and physiological changes that characterize neonates and affect their response to pharmacological treatments, neonatal pharmacology is different from children and adults and deserves particular attention. Although preterms are usually considered part of the neonatal population, they have physiological and pharmacological hallmarks different from full-terms and, therefore, need specific considerations. Antibiotics are widely used among preterms. In fact, during their stay in neonatal intensive care units (NICUs), invasive procedures, including central catheters for parental nutrition and ventilators for respiratory support, are often sources of microbes and require antimicrobial treatments. Unfortunately, the majority of drugs administered to neonates are off-label due to the lack of clinical studies conducted on this special population. In fact, physiological and ethical concerns represent a huge limit in performing pharmacokinetic (PK) studies on these subjects, since they limit the number and volume of blood sampling. Therapeutic drug monitoring (TDM) is a useful tool that allows dose adjustments aiming to fit plasma concentrations within the therapeutic range and to reach specific drug target attainment. In this review of the last ten years’ literature, we performed Pubmed research aiming to summarize the PK aspects for the most used antibiotics in preterms.

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Section: Therapeutic Drug Monitoring (Tdm) Of Antibiotics In Pretermsmentioning

confidence: 99%

Use of Antibiotics in Preterm Newborns

et al. 2022

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“…The assigned chromatographic conditions gave the best results regarding selectivity and accuracy. Both methods are considered to be economic and suitable for routine low-cost analysis when compared to other hyphenated methods such as GC-MS, GC TQ-MS and LC-MS/MS [43][44][45][46] .…”

Section: Quechers and Chromatographic Conditionsmentioning

confidence: 99%

Appraisal for Edible Use of Vegetable Crops Cultivated in Egypt after Treatment with Selected Insecticides and Fungicides: Insights of Dissipation Rates and Pre-harvest Intervals

2021

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An analytical investigation was carried out to study the dissipation rate of two commonly used pesticides, Thiamethoxam (neonicotinoid insecticide) and propamocarb hydrochloride (carbamate fungicide) applied to four vegetable crops: cucumber, zucchini, lettuce and pepper, after openfield application. Samples were harvested according to a scheduled plan followed by QuEChERS extraction, then thiamethoxam residues were analyzed using a GC-ECD method, while propamocarb HCl residues were analyzed using an HPLC-UV method. Validation parameters were attained for both methods and the kinetic profile was studied which fitted the first -order kinetics where k, t ½ and t90 were calculated. The proper pre-harvest interval (PHI) was studied for each crop to ensure that the residues levels declined to reach below the maximum residue limit (MRL) where the crop is suitable for edible use. These values were found to be different from labelled values which proves that the PHIs are greatly affected by changing weather conditions.

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“…According to the literature survey, some techniques were reported for the estimation of VANH in different matrices including spectrophotometric [10][11][12], spectrofluorimetric [13][14][15], electrochemical [7,16,17], HPLC [18][19][20][21][22][23][24][25][26], LC-MS/MS [8,[27][28][29][30][31][32][33][34][35][36][37], capillary electrophoresis [38][39][40][41], radioimmunoassay and ELISA-based immunoassay techniques [42][43][44]. VANH has native fluorescence at 335 nm after excitation at 268 nm according to the previous study [14].…”

Section: Introductionmentioning

confidence: 99%

Utility of Silver-nanoparticles for Nano-fluorimetric Determination of Vancomycin Hydrochloride in Pharmaceutical Formulation and Biological Fluids: Greenness Assessment

Mohamed

2022

J Fluoresc

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Vancomycin hydrochloride (VANH) is a glycopeptide antibiotic commonly employed in the prophylaxis and therapy of various gram-positive bacterial life-threatening infections. Due to the narrow therapeutic window of VANH, its serum levels should be well-monitored to avoid its toxicity and to optimize its therapy. Herein, an innovative silver-nanoparticles enhanced fluorescence technique was designed for VANH rapid analysis in its pharmaceutical formulation and biological fluids. This technique is based on reinforcement of VANH fluorescence intensity with silver-nanoparticles that were synthesized by a redox reaction between VANH and silver nitrate in NaOH alkaline medium using polyvinylpyrrolidone as a stabilizer. The produced silver-nanoparticles were characterized by using UV–visible spectroscopy where they have an intense absorption maximum at 415 nm and transmission electron microscope (TEM) micrograph where they are spherical in shape with smooth surface morphology and size of 10.74 ± 2.44 nm. The fluorescence intensity was measured at 394 nm after excitation at 259 nm. Under optimum conditions, a good linear relationship was accomplished between the VANH concentration and the fluorescence intensity in a range of (1–36) ng/mL with a limit of detection of 0.29 ng/mL. Greenness assessment was performed using two assessment tools namely; eco-scale scoring and green analytical procedure index revealing excellent greenness of the proposed technique. The proposed technique was validated according to the International Conference on Harmonisation (ICH) recommendations and statistically compared with the reported HPLC method revealing no significant difference concerning accuracy and precision at p = 0.05. The proposed technique depended primarily on water as a cheap and eco-friendly solvent.

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Validation of a simple and economic HPLC-UV method for the simultaneous determination of vancomycin, meropenem, piperacillin and tazobactam in plasma samples

Cited by 38 publications

References 22 publications

Use of Antibiotics in Preterm Newborns

Use of Antibiotics in Preterm Newborns

Appraisal for Edible Use of Vegetable Crops Cultivated in Egypt after Treatment with Selected Insecticides and Fungicides: Insights of Dissipation Rates and Pre-harvest Intervals

Utility of Silver-nanoparticles for Nano-fluorimetric Determination of Vancomycin Hydrochloride in Pharmaceutical Formulation and Biological Fluids: Greenness Assessment

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