Validation of a Salmonella loop-mediated isothermal amplification assay in animal food

Domesle, Kelly J.; Yang, Qianru; Hammack, Thomas S.; Ge, Beilei

doi:10.1016/j.ijfoodmicro.2017.10.020

Cited by 31 publications

(34 citation statements)

References 46 publications

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“… Monitoring methods used to detect LAMP amplicons. (a) Naked eye observation based on white precipitate (Hara-Kudo et al , 2005 ), DNA dye (SYBR Green I) (Mashooq et al , 2016 ), and colorimetric indictor (calcein) (Li et al , 2016 ), respectively; (b) gel electrophoresis (Hara-Kudo et al , 2005 ); (c) real-time turbidity (Domesle et al , 2018 ); (d) real-time fluorescence (Domesle et al , 2018 ); (e) BART (Yang et al , 2016 ); (f) ELISA (Ravan and Yazdanparast, 2012); (g) LFD (Zhao et al , 2017 ); and (h) electrochemical method (Hsieh et al , 2012 ). BART, bioluminescent assay in real-time; ELISA, enzyme-linked immunosorbent assay; LAMP, loop-mediated isothermal amplification; LFD, lateral flow dipstick.…”

Section: Salmonella Lamp Assay Developmentmentioning

confidence: 99%

“…enterica strains of 39 serovars were shown at the time of assay development (Hara-Kudo et al , 2005 ). Very recently, Domesle et al ( 2018 ) evaluated the specificity of our invA -based Salmonella LAMP assay (Yang et al , 2016 ) ( Fig. 2 ) using 300 bacterial strains (247 Salmonella strains of 185 serovars and 53 non- Salmonella strains) and demonstrated 100% specificity on both turbidimetry- and fluorescence-based platforms.…”

Section: Salmonella Lamp Assay Developmentmentioning

confidence: 99%

“…arizonae strains were tested and when compared to those belonging to other S. enterica subspecies, significantly longer time-to-positive results were required for these S. enterica subsp. arizonae strains (Domesle et al , 2018 ).…”

Section: Salmonella Lamp Assay Developmentmentioning

confidence: 99%

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Loop-Mediated Isothermal Amplification forSalmonellaDetection in Food and Feed: Current Applications and Future Directions

Yang

Domesle

2018

Foodborne Pathogens and Disease

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Loop-mediated isothermal amplification (LAMP) has become a powerful alternative to polymerase chain reaction (PCR) for pathogen detection in clinical specimens and food matrices. Nontyphoidal Salmonella is a zoonotic pathogen of significant food and feed safety concern worldwide. The first study employing LAMP for the rapid detection of Salmonella was reported in 2005, 5 years after the invention of the LAMP technology in Japan. This review provides an overview of international efforts in the past decade on the development and application of Salmonella LAMP assays in a wide array of food and feed matrices. Recent progress in assay design, platform development, commercial application, and method validation is reviewed. Future perspectives toward more practical and wider applications of Salmonella LAMP assays in food and feed testing are discussed.

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Section: Salmonella Lamp Assay Developmentmentioning

confidence: 99%

Section: Salmonella Lamp Assay Developmentmentioning

confidence: 99%

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Loop-Mediated Isothermal Amplification forSalmonellaDetection in Food and Feed: Current Applications and Future Directions

Yang

Domesle

2018

Foodborne Pathogens and Disease

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“…(Department of Medical Sciences Thailand, ). Regarding the spiked chicken meat samples, the analytical sensitivity of the real‐time LAMP assay after 6 hr of preincubation was comparable to that of the LAMP assays for Salmonella in spiked vegetables and fruits as 1.1–2.9 CFU/25 g (Yang et al, ) and six Salmonella serovars in spiked animal food as 1.3–28 CFU/25 g (Domesle, Yang, Hammack, & Ge, ). Nevertheless, Li et al () have developed a visual LAMP assay based on gene62181533 of Salmonella spp.…”

Section: Discussionmentioning

confidence: 86%

Development and evaluation of real‐time loop mediated isothermal amplification assay for rapid and sensitive detection of Salmonella spp. in chicken meat products

Arunrut

Kiatpathomchai

Ananchaipattana

2018

Journal of Food Safety

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Salmonella spp., one of the most prevalent bacterial causes of foodborne disease, is a major public health concern due to its common occurrence in chicken meat products. The detection of Salmonella spp. using conventional culturing methods requires an excessive amount of time and effort. Thus, in response to the need for a rapid, sensitive, and convenient method for Salmonella detection, a quantitative assay using real‐time Loop‐mediated isothermal amplification (real‐time LAMP) was developed using gene62181533 as the target sequence amplified. The real‐time LAMP assay does not show cross‐reactivity with several other common bacterial pathogens and the detection limit for genomic DNA in pure culture was found to be 1.2 CFU/ml. Testing with spiked samples simultaneously detected 7 CFU/ml of Salmonella pathogen in the artificially inoculated samples after enrichment for 6 hr. In comparison with the standard culture‐based methods in the analysis of 120 raw chicken meat samples, the results of the sensitivity, accuracy, and specificity tests of the real‐time LAMP assay were 94.02, 90.83, and 86.79%, respectively. These results indicate that this method is not only sensitive and specific but can also be used for rapid detection and differentiation of foodborne disease‐causing bacteria. Practical applications Salmonella is an important bacterial genus which causes most of the common foodborne illnesses worldwide. The disease mainly caused by Salmonella spp. through consumption of contaminated eggs and poultry products is salmonellosis which can manifest as bacterial diarrhea through to septicemia. The result indicates the potential usefulness of real‐time LAMP assay based on the gene62181533 for a rapid, specific, sensitive, and quantitative assay for Salmonella detection in food products. This assay offers a low cost quantitative method in the clinical diagnosis of Salmonella in resource‐limited health‐care facilities and clinical laboratories in developing countries and in field tests.

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“…The diagnostic workflow developed here can be easily adapted to detect the presence of target genes from different food pathogens using highly specific LAMP primer sets readily available in the literature [37–39]. Unlike specificity, the sensitivity of the primers is less critical to the success of the assay as the overnight enrichment step significantly increases the pathogen levels in the tested sample.…”

Section: Discussionmentioning

confidence: 99%

Development and on-site evaluation of an easy-to-perform and low-cost food pathogen diagnostic workflow for low-resource communities

Mason

Botella

2020

Preprint

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20Food-borne diseases are a leading cause of illness and death in many developing 21 countries and thus, there is a real need to develop affordable and practical 22 technologies that can help improve food safety in these countries. The ability to 23 efficiently identify food pathogens is essential to allow national regulatory authorities 24 to monitor food quality and implement safety protocols. In this study, we have 25 developed a simple, low-cost ($0.76 (USD)) complete food pathogen diagnostic 26 workflow ideally suited for deployment in low-resource environments that uses a 27 simple four step process (sample enrichment, cell lysis, DNA amplification, and 28 naked-eye readout). The minimal number of steps and equipment involved in our 29 diagnostic workflow, as well as the simplicity of the yes/no flocculation readout, 30 allows non-technical personnel to perform and interpret the assay. To evaluate the 31 system's performance, we tested the entire system on fresh produce samples 32 collected from local farms and markets in Cambodia for the presence of the E. coli 33 O157 O-antigen polymerase, wzy. Although this was a proof-of-concept study, our 34 system successfully revealed a clear correlation between the origin and condition of 35 the produce collected and their likelihood of contamination. In conclusion, we believe 36 that our easy-to-perform diagnostic system can have a significant impact on 37 improving food quality and human health if adopted by regulatory authorities in 38 developing countries due to the assay's simplicity, affordability, and adaptability. 3 40 Introduction 41Over the last 20 years, there has been a significant increase in the incidence of food-42 borne diseases worldwide [1]. The risk of infection by a food-borne disease is 43 significantly higher in developing countries due to a combination of factors including 44 access to clean water and bathroom facilities, poor hygiene education, inadequate 45 food production and storage practices, and either insufficient food safety legislation 46 or poor implementation of existing legislation [2]. Most food-borne disease infections 47 in these countries result from the consumption of perishable foods sold in informal 48 markets [3] and, as such, food-borne diseases have become a leading cause of 49 illness and death in developing countries [4, 5]. Hence, there is a need for low-cost 50 and simple technologies that can help health authorities to monitor and enforce 51 adequate levels of food safety. In addition to the health benefits, increased food 52 safety standards would likely have economic benefits as a result of increased 53 demand for fresh produce exports [3]. 54 55 Shiga toxin-producing Escherichia coli (STEC) are a class of E. coli responsible for 56 many food-borne outbreaks and sporadic cases of gastrointestinal illness, with a 57 range of symptoms including haemorrhagic colitis (stomach cramping and bloody 58 diarrhoea) and the potentially fatal hemolytic-uremic syndrome (break down of red 59 blood cells, kidney failure, re...

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Validation of a Salmonella loop-mediated isothermal amplification assay in animal food

Cited by 31 publications

References 46 publications

Loop-Mediated Isothermal Amplification forSalmonellaDetection in Food and Feed: Current Applications and Future Directions

Loop-Mediated Isothermal Amplification forSalmonellaDetection in Food and Feed: Current Applications and Future Directions

Development and evaluation of real‐time loop mediated isothermal amplification assay for rapid and sensitive detection of Salmonella spp. in chicken meat products

Development and on-site evaluation of an easy-to-perform and low-cost food pathogen diagnostic workflow for low-resource communities

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