Validation of a refined short-term adult fish reproductive test with improved power for mummichog (Fundulus heteroclitus) to test complex effluents

Bosker, Thijs; Hewitt, L. Mark; Munkittrick, Kelly R.; MacLatchy, Deborah L.

doi:10.1016/j.ecoenv.2010.07.037

Cited by 8 publications

(4 citation statements)

References 32 publications

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“…Short-term reproductive bioassays are used to quantitate the effects of EDCs on fish reproduction and endocrine-related biomarkers. In Fundulus these studies have been developed and optimized using primarily 17␣-ethynylestradiol as the stressor (Blewett et al, 2013;Bosker et al, 2009Bosker et al, , 2010Doyle et al, 2013;MacLatchy et al, 2003). Yet there is evidence both from wild fish populations (Bugel et al, 2010;Pait and Nelson, 2009) and mammalian studies that PAHs, including BaP, also adversely impact reproductive potential.…”

Section: Discussionmentioning

confidence: 99%

“…The following study utilized Fundulus heteroclitus in a shortterm waterborne exposure in order to assess if BaP's published effects on aromatase modulation would translate into reproductive deficits. The experiment was adapted from previously described studies (Bosker et al, 2010;MacLatchy et al, 2003;Peters et al, 2007) in order to quickly observe potential impacts of BaP exposure to reproductive and steroidogenic endpoints.…”

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confidence: 99%

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Benzo[a]pyrene Effects on Reproductive Endpoints in Fundulus heteroclitus

Booc

Thornton

Lister

et al. 2014

Toxicological Sciences

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Benzo[a]pyrene (BaP) is a polycyclic aromatic hydrocarbon (PAH) that has been implicated in modulating aromatase enzyme function with the potential to interrupt normal reproductive function. The aim of this study was to use a fish model, Fundulus heteroclitus, to assess whether BaP exposure adversely impacts reproduction. Adult fish were exposed to waterborne BaP nominal concentrations of (0, 1, or 10 μg/l) for 28 days. Males and females were combined for the second half of the exposure (days 14-28) in order to quantitate egg production and fertilization success. Egg fertilization and subsequent hatching success of F1 embryos was significantly decreased by the high dose of BaP. In males, both gonad weight and plasma testosterone concentrations were significantly reduced compared to controls by 10 μg/l BaP. Histopathological examination of testes including spermatogonia, spermatocyte and spermatid cyst areas, percentage of cysts per phase, and area of spermatozoa per seminiferous tubule were not significantly affected. Other biomarkers, including male liver weight, liver vitellogenin (vtg) mRNA expression and sperm concentrations, were also not affected. In females, estradiol concentrations were significantly reduced after BaP exposure, but egg production, gonad weight, liver weight, vtg expression and oocyte maturation were not altered. Steroid concentrations in Fundulus larvae from exposed parents at 1 and 3 weeks posthatch were not significantly changed. BaP exposure at these environmentally relevant concentrations caused negative alterations particularly in male fish to both biochemical and phenotypic biomarkers associated with reproduction and multigenerational embryo survival.

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Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

Benzo[a]pyrene Effects on Reproductive Endpoints in Fundulus heteroclitus

Booc

Thornton

Lister

et al. 2014

Toxicological Sciences

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“…In July of 2009, mummichog were exposed using a modified and validated short‐term reproductive test, to optimize statistical power to detect differences . Fish were held in 16‰ saline water at 18 °C to 20 °C, under a summer photoperiod (16:8‐h light:dark) at >80% dissolved oxygen.…”

Section: Methodsmentioning

confidence: 99%

Mummichog (Fundulus heteroclitus) continue to successfully produce eggs after exposure to high levels of 17α‐ethinylestradiol

Bosker

Munkittrick

Lister

et al. 2016

Enviro Toxic and Chemistry

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17α-Ethinylestradiol (EE2) is a potent estrogen used in birth-control pills. Previous laboratory and field studies have shown negative impacts in a variety of fish species after exposure to low levels of EE2, most notably a nearly complete shutdown of egg production. The present study demonstrates that mummichog (Fundulus heteroclitus), a small-bodied estuarine species, is able to continue to produce eggs after exposure for 28 d to 100 ng of EE2/L. No effect of EE2 on egg production was observed, whereas a >35-fold increase in vitellogenin (vtg 1) gene expression in males was found. The lack of response in egg production in fish exposed to high levels of EE2 warrants further investigations on species-specific responses to estrogens and endocrine disruptors in general.

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“…Due to their euryhaline tolerances, mummichog are a model species providing the ability to test impacts of EDCs across a range of salinities. Studies using the northern subspecies of mummichog (F. h. macrolepidotus) have resulted in standardized reproductive endocrine bioassays (MacLatchy et al, 2003) and adult fish reproductive tests (Peters et al, 2007;Bosker et al, 2010), which have been used to assess the impacts of complex effluents (e.g., pulp mill; Bosker et al, 2009), environmental contaminants (including EE 2 ; Bosker et al, 2016), or other model compounds (e.g., androgens; Glinka et al, 2015;Rutherford et al, 2015). A suite of relevant endpoints, spanning various levels of biological organization have been used to assess the effects of EE 2 on reproductive function in mummichog.…”

Section: Introductionmentioning

confidence: 99%