Validation of a point-of-care handheld blood total calcium analyzer in postpartum dairy cows

Serrenho, Rita Couto; Bruinjé, T.C.; Morrison, Emma I.; Renaud, D.L.; DeVries, T.J.; Duffield, T.F.; LeBlanc, S.J.

doi:10.3168/jdsc.2020-0006

Cited by 4 publications

(5 citation statements)

References 21 publications

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“…In our study, the main objective was to compare the performance of a tCa POC device with the gold standard method to measure bovine blood tCa. The tCa concentration correlation between gold standard and the POC device is comparable to previous validations of different Ca POC ( Peiró et al, 2010 ; Couto Serrenho et al, 2021 ; Suzuki et al, 2021 ). The sensitivity, specificity, PPV, and NPV of the POC device in plasma and serum are summarized in Table 1 .…”

supporting

confidence: 83%

“…The accuracy (95% CI) of the POC device was 95.3% (90.6, 98.1) and 92.7% (87.3, 96.3) in serum and plasma, respectively. The PPV, NP, and accuracy of the test were greater in the current study compared with a similar device recently analyzed ( Couto Serrenho et al, 2021 ).…”

contrasting

confidence: 56%

“…Our results are similar to previous studies intending validation of POC Ca in whole blood, plasma, and serum. Unfortunately, the precision and accuracy of these POC devices to measure blood Ca for field or laboratory use are inconsistent ( Neves et al, 2018b ; Couto Serrenho et al, 2021 ).

Figure 1 Linear regression analysis between total calcium (tCa; mmol/L) blood concentration measured with the gold standard method at the New York Animal Health Diagnostic Center and (a) tCa plasma concentration with the tCa point-of-care compact analyzer (ARKRAY Inc.); (b) tCa serum concentration with the Ca point-of-care compact analyzer (ARKRAY Inc.).…”

mentioning

confidence: 99%

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Evaluation of a point-of-care calcium device in bovine plasma and serum

et al. 2023

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Graphical Abstract Summary: Serum and plasma from dairy cows were collected to measure total calcium (Ca) concentration using the Ca point-of-care compact analyzer (POC; ARKRAY Inc.) device and a gold standard method. The performance of the POC analyzer was estimated using correlation coefficients (Spearman), coefficient of variation (%), sensitivity, specificity, Passing and Bablok regression, and Bland-Altman agreement test. We found a strong correlation between the POC device and the gold standard method for total Ca plasma and serum. However, the clinical application of the POC device should be carefully considered because its ability to detect cows with hypocalcemia in serum or plasma samples was poor.

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supporting

confidence: 83%

contrasting

confidence: 56%

mentioning

confidence: 99%

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Evaluation of a point-of-care calcium device in bovine plasma and serum

et al. 2023

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“…Because there is no consensus in the literature on the best cut-off for defining SHC (Couto Serrenho et al, 2021), we assessed the effect of the AcD treatment on SHC using a threshold of plasma Ca < 2.0 mmol/l, which was based on the reference range of values in the Merck Veterinary Manual. Ideally, the threshold for defining SHC should be based on measured effects on outcomes of interest (Couto Serrenho et al, 2021), such as disease incidence, reproductive performance, or culling risk. However, this approach can lead to the use of different thresholds for different parameters, depending on the outcome of interest, cow parity, and the time of sampling.…”

Section: Discussionmentioning

confidence: 99%

Acidified diet is not effective in preventing hypocalcemia in dairy cows on French commercial farms

2022

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The objective of this study was to assess, in the context of typical dairy farms in western France, the preventive effects of prepartum diet acidification (AcD) and mineral intake during late gestation on the incidence of subclinical hypocalcemia (SHC) and subclinical hypophosphatemia (SHP) after calving. We conducted a longitudinal study that followed a cohort of 371 Holstein cows from 26 French dairy farms; of these, 235 cows (15 farms) were supplied with anionic salts during late gestation, and 136 cows (11 farms) were not. Blood samples were collected from 1 to 22 cows per farm (average of 14.3 cows per farm) between 24 and 48 h after calving. Total calcium and inorganic phosphorus concentrations in plasma were then quantified by inductively coupled plasma - optical emission spectrometry. The effects of AcD on the incidence of SHC and SHP were assessed using mixed linear models that evaluated the cow-level factors parity, milk yield index, and individual health events/treatments, and the farm-level factors diet calcium and magnesium content, dietary phosphorus intake, vitamin D supply, diet crude protein content, and duration of AcD, with farm as a random effect. Approximately 55% of cows were diagnosed with SHC (calcium < 2.0 mmol/l, n = 203) and 37% with SHP (inorganic phosphorus < 1.3 mmol/l, n=136). The first model confirmed earlier findings that the risk of SHC is higher with increased parity (P ≤ 0.0001) and revealed a higher risk associated with high milk yield (P = 0.0005), high phosphorus intake (40–60 g/cow per day, OR = 3.5; ≥ 60 g/cow per day, OR = 7.3; P = 0.0003) and high vitamin D supply (≥ 19950 IU/cow per day, OR = 3, P = 0.007). The second model highlighted a greater risk of SHP with increasing parity (P = 0.03) and showed trends for the preventive effects of AcD (OR = 0.4, P = 0.07) and moderate amounts of phosphorus in the diet (OR = 0.4, P = 0.10). Overall, our results do not support the effectiveness of AcD in preventing SHC or SHP under field conditions examined here, probably resulting from insufficient diet acidification.

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“…Blood collection for assessment of early lactation dairy cows is not uncommon for other metabolic disorders like hyperketonemia and hypocalcemia [ 9 , 10 , 11 ] and may represent an opportunity to assess liver TG content or functionality. However, the previously mentioned -emia disorders are defined by quantification of a blood biomarker, whereas bFLS is defined by a liver tissue measurement.…”

Section: Introductionmentioning

confidence: 99%

Prediction of Liver Triglyceride Content in Early Lactation Multiparous Holstein Cows Using Blood Metabolite, Mineral, and Protein Biomarker Concentrations

Pralle

Holdorf

Oliveira

et al. 2022

Animals

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Bovine fatty liver syndrome (bFLS) is difficult to diagnose because a liver tissue biopsy is required to assess liver triglyceride (TG) content. We hypothesized that a blood biomarker panel could be a convenient alternative method of liver TG content assessment and bFLS diagnosis. Our objectives were to predict liver TG using blood biomarker concentrations across days in milk (DIM; longitudinal, LT) or at a single timepoint (ST; 3, 7, or 14 DIM), as well as different biomarker combination based on their perceived accessibility. Data from two separate experiments (n = 65 cows) was used for model training and validation. Response variables were based on the maximum liver TG observed in 1 and 14 DIM liver biopsies: Max TG (continuous), Low TG (TG > 13.3% dry matter; DM), Median TG (TG > 17.1% DM), and High TG (TG > 22.0% DM). Model performance varied but High TG was well predicted by sparse partial least squares—discriminate analysis models using LT and ST data, achieving balanced error rates ≤ 15.4% for several model variations during cross-validation. In conclusion, blood biomarker panels using 7 DIM, 14 DIM, or LT data may be a useful diagnostic tool for bFLS in research and field settings.

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Validation of a point-of-care handheld blood total calcium analyzer in postpartum dairy cows

Cited by 4 publications

References 21 publications

Evaluation of a point-of-care calcium device in bovine plasma and serum

Evaluation of a point-of-care calcium device in bovine plasma and serum

Acidified diet is not effective in preventing hypocalcemia in dairy cows on French commercial farms

Prediction of Liver Triglyceride Content in Early Lactation Multiparous Holstein Cows Using Blood Metabolite, Mineral, and Protein Biomarker Concentrations

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