Validation of a phenol‐sulfuric acid method in a microplate format for the quantification of soluble sugars in ruminant feeds

Kondo, Makoto; Mulianda, Randi; Matamura, Masaya; Shibata, Toshiyuki; Mishima, Takashi; Jayanegara, Anuraga; Isono, Naoto

doi:10.1111/asj.13530

Cited by 16 publications

(10 citation statements)

References 39 publications

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“…This method is carried out to determine the appropriate concentration to obtain the highest absorbance that can be read on a UV-Vis Spectrophotometer [18,19]. The test was carried out three times using several comparisons of Fucoidan concentration, 5% phenol and sulfuric acid as shown in Table 1 and Table 2.…”

Section: Determination Of Phenol-sulfuric Acid Concentration For Fuco...mentioning

confidence: 99%

Ex Vivo and In Vivo Retention Time Evaluation of Fucoidan Isolated from Macrocystis pyrifera Through a Thermosensitive Gel System in The Vaginal Route

Sam,

Marzaman,

Mudjahid

et al. 2024

BIO Web Conf.

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This study evaluated Fucoidan from Macrocystis pyrifera as a potential treatment for cervical cancer. The research aimed to examine Fucoidan’s in vivo retention capacities in poloxamer-based in situ gels for vaginal drug delivery systems. Five different thermosensitive gel formulations were developed, each with varying concentrations of Pluronic F127 and F68 polymers. The incorporation of HPMC affected the gelation temperature, viscosity, and bioadhesive strength. The accepted formula, F3, had a bioadhesive value of 5415.93 ± 98.74 dyne/cm2 and could form a gel at physiological temperature. Ex vivo animal models showed that Fucoidan components retained well on vaginal tissue. Only F1, F2, and F3 achieved the media after 8 hours of examination. In vivo evaluation showed F3 had the highest drug concentration retained in the vaginal mucosa of female rats after 8 hours (24,115 ± 4,842 g), slowly removed after 24 hours (13,014 ± 5,596 g). In conclusion, increases in the hydrophilic content of formulations led to the retained hydrogel formula, which increased drug release and lowered intravaginal elimination.

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Section: Determination Of Phenol-sulfuric Acid Concentration For Fuco...mentioning

confidence: 99%

Ex Vivo and In Vivo Retention Time Evaluation of Fucoidan Isolated from Macrocystis pyrifera Through a Thermosensitive Gel System in The Vaginal Route

Sam,

Marzaman,

Mudjahid

et al. 2024

BIO Web Conf.

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“…The levan content was measured by the phenol-sulfuric acid method (PSA) with minor modifications (Kondo et al, 2021). In brief, 1 mL of natto mucus (diluted to a certain number) was mixed with 1 mL of distilled water, 1 mL of 5% phenol solution and 5 mL of concentrated sulfuric acid.…”

Section: Determination Of Mucus Composition Of Nattomentioning

confidence: 99%

Correlation analysis between soluble sugars of soybean and mucus drawing length of fermented natto

et al. 2022

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Natto is a traditional fermented food from Japan that is made from soybean fermented by Bacillus subtilis natto. The mucus drawing length of natto determines the sensory quality of natto, and there are differences in the mucus drawing length and sensory quality of natto fermented with soybeans with different contents of soluble sugars. In this study, soybeans with different contents of soluble sugars were used to ferment natto, and the differences and correlations between the mucilage composition, the mucus drawing length and the sensory quality of natto were investigated. The contents of oligosaccharides and total sugars in soybean were significantly positively correlated with the mucus drawing length (R = 0.921, R = 0.956), and the correlation between the drawing length of mucilage and total sugars was stronger. Fermenting soybeans with high contents of total sugars could make mucus drawing of natto longer. The contents of oligosaccharides and total sugars in soybean were significantly positively correlated with the sensory index score (R = 0.886, R = 0.925). The natto fermented from soybean with high contents of soluble total sugars had better overall sensory quality. Soluble sugars had a positive influence on mucus drawing length and sensory quality of natto.

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“…The NIR spectral data were combined with those of a colorimetric method that employs a phenol-sulfuric acid (PSA) assay in microplate format to quantify carbohydrates. 32 This method is used widely in food, algae, 33 mushrooms 34 and feed [35][36][37] because of its sensitivity and simplicity.…”

Section: Introductionmentioning

confidence: 99%

Nutritional labelling of food products purchased from online retail outlets: screening of compliance with European Union tolerance limits by near infrared spectroscopy

Bragolusi

Tata

Massaro

et al. 2023

Journal of Near Infrared Spectroscopy

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Nutritional information provided on food labels can impact healthy dietary decisions of consumers. The accuracy of the information provided is of paramount importance to guide consumers’ food choices and prevent food-related chronic diseases. The present study aimed to verify the veracity of nutritional labels of 103 food products purchased online through well-known e-commerce websites (80 processed and 23 unprocessed items) using near infrared spectroscopy. Among processed food products, surprisingly, 28 food products out of 80 (35%) did not bear nutritional labels. Considering the European tolerances for nutrient values declared on a label, the comparison of experimental values with those reported on the labels showed that more than 74% of the values declared on the label were congruent with the NIR experimental data, whereas 7.5% of the label values were non-compliant with the tolerance limits, and about 11.3% were slightly outside the tolerance limits. Note that 6.6% of the values indicated in the labels did not abide the regulation at all. Finally, 35.8% of the samples showed at least one value outside the tolerance limits. The current study demonstrated the capability of NIR spectroscopy for monitoring the compliance of nutritional labels with EU tolerance limits and guiding the choice of reference methods for further confirmation purposes.

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Validation of a phenol‐sulfuric acid method in a microplate format for the quantification of soluble sugars in ruminant feeds

Cited by 16 publications

References 39 publications

Ex Vivo and In Vivo Retention Time Evaluation of Fucoidan Isolated from Macrocystis pyrifera Through a Thermosensitive Gel System in The Vaginal Route

Ex Vivo and In Vivo Retention Time Evaluation of Fucoidan Isolated from Macrocystis pyrifera Through a Thermosensitive Gel System in The Vaginal Route

Correlation analysis between soluble sugars of soybean and mucus drawing length of fermented natto

Nutritional labelling of food products purchased from online retail outlets: screening of compliance with European Union tolerance limits by near infrared spectroscopy

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