“…The currently accepted methods for the detection of Trypanosoma such as microscopic examination and xenodiagnoses have poor sensitivity and are also labor-intensive and time-consuming [ 1 , 2 ]. Immunological methods such as enzyme-linked immunosorbent assay, immunochromatographic dipstick test, radioimmunosorbent assay, and immunofluorescence antibody test are rapid and sensitive but not specific [ 3 , 4 , 5 , 6 , 7 , 8 , 9 ]. Molecular methods such as PCR and real-time nucleic acid sequence-based amplification are very specific but expensive and time consuming, although combination of PCR and chemiluminescence southern blot has been used to improve the sensitivity of the detection of Trypanosoma [ 10 , 11 , 12 , 13 , 14 , 15 , 16 , 17 ].…”