Validation of a one-step extraction/methylation method for determination of fatty acids and cholesterol in marine tissues

Meier, Sonnich; Mjøs, Svein A.; Joensen, Hóraldur; Grahl‐Nielsen, Otto

doi:10.1016/j.chroma.2005.11.045

Cited by 144 publications

(96 citation statements)

References 42 publications

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“…phosphatidylcholine (PC) from egg yolk lecithin (95% PC), 1,2-dioleoylphosphatidylglycerol (DOPG), porcine brain phosphatidylserine (PBPS), 1-stearoyl-2-docosahexaenoylphosphatidylserine (SDPS), 1,2-dipalmitoylphosphatidylcholine (DPPC), 1,2-dimyristoylphosphatidylcholine (DMPC), 1,2-dihexanoylphosphatidylcholine (DHPC), and 1,2-dimyristoylphosphatidylserine (DMPS) were purchased from Avanti Polar Lipids, Inc., except egg yolk PC, which was from Sigma. The absence of fatty acid oxidation both in natural extracts and pure synthetic species was kindly verified by Sonnic Meier as reported (38).…”

Section: Methodsmentioning

confidence: 99%

Three-way Interaction between 14-3-3 Proteins, the N-terminal Region of Tyrosine Hydroxylase, and Negatively Charged Membranes

Halskau

Ying

Baumann

et al. 2009

Journal of Biological Chemistry

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Tyrosine hydroxylase (TH), the rate-limiting enzyme in the synthesis of catecholamines, is activated by phosphorylationdependent binding to 14-3-3 proteins. The N-terminal domain of TH is also involved in interaction with lipid membranes. We investigated the binding of the N-terminal domain to its different partners, both in the unphosphorylated (TH-(1-43)) and Ser 19 -phosphorylated (THp-(1-43)) states by surface plasmon resonance. THp-(1-43) showed high affinity for 14-3-3 proteins (K d ϳ 0.5 M for 14-3-3␥ and -and 7 M for 14-3-3 ). The domains also bind to negatively charged membranes with intermediate affinity (concentration at half-maximal binding S 0.5 ‫؍‬ 25-58 M (TH-(1-43)) and S 0.5 ‫؍‬ 135-475 M (THp-(1-43)), depending on phospholipid composition) and concomitant formation of helical structure. 14-3-3␥ showed a preferential binding to membranes, compared with 14-3-3 , both in chromaffin granules and with liposomes at neutral pH. The affinity of 14-3-3␥ for negatively charged membranes (S 0.5 ‫؍‬ 1-9 M) is much higher than the affinity of TH for the same membranes, compatible with the formation of a ternary complex between Ser 19 -phosphorylated TH, 14-3-3␥, and membranes. Our results shed light on interaction mechanisms that might be relevant for the modulation of the distribution of TH in the cytoplasm and membrane fractions and regulation of L-DOPA and dopamine synthesis.

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Section: Methodsmentioning

confidence: 99%

Three-way Interaction between 14-3-3 Proteins, the N-terminal Region of Tyrosine Hydroxylase, and Negatively Charged Membranes

Halskau

Ying

Baumann

et al. 2009

Journal of Biological Chemistry

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“…TAGs were separated from the total lipid by high-performance thin-layer chromatography (HPTLC) using the neutral solvent system of Olsen & Henderson (1989). All samples were methylated, and the respective fatty acid methyl esters (FAME) were analysed on a HP-7890A gas chromatograph (Agilent) with a flame ionization detector (GC-FID) as described previously in Olsen et al (2004) for the feeding experiment, and in Meier et al (2006) for the field trial. In total, 61 well-defined peaks in the chromatogram were selected, and identified by comparing retention times with a FAME standard (GLC-463 from Nu-Chek Prep) and retention index maps and mass spectral libraries (GC-MS) (www.chrombox.…”

Section: Fa Analysismentioning

confidence: 99%

Use of fatty acid profiles to monitor the escape history of farmed Atlantic salmon

Skilbrei¹,

Normann²,

Meier³

et al. 2015

Aquacult. Environ. Interact.

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Farmed Atlantic salmon can escape from fish farms at various stages of their life, from juveniles to large mature fish. Escapees that enter rivers to spawn pose a threat to the genetic integrity of wild populations. Knowledge about the timing of these escapes can provide important information for wildlife management and the aquaculture industry, enabling them to prevent or mitigate the negative impacts of escapees. Farmed salmon food has a high content of terrestrial lipids; thus, we used fatty acid (FA) profiling to monitor the escape history of farmed salmon. Escaped salmon captured in rivers (n = 251) presented a wide range of FA profiles that we used to classify the fish as (1) early-escaped wild-like fish that were assumed to have escaped at smolt or early post-smolt stage (24%), (2) recently escaped fish with high levels of FAs typically found in commercial salmon food (61%) and (3) intermediate escapees whose FA profiles lay between those 2 groups (15%). To estimate the size at escape of the intermediate escapees, we performed a feeding experiment that monitored the development of FA profiles after a shift in diet from terrestrial to marine lipids. Most intermediate escapees appeared to have escaped when they were < 3 kg, and ranged from 3 to 11 kg when recaptured in rivers. We conclude that FA profiling is a promising tool to monitor escape histories, and that the proportion of post-smolt escapees in this study was high compared to official escape statistics which include very few reports of young fish escaping.

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“…The application of chemical ionisation as opposed to electron impact ionisation allowed the analysis of a large number of chemically defined metabolites from the complex biological matrix [14]. Owing to the hypothesis-generating nature of the screening experiment and in order to avoid the introduction of contaminants, no antioxidant or chelate forming additives were used and a single step method of extraction/methylation [9][10][11] was used, that minimized the number of sample handling steps. Larger samples sizes and improved technologies will lead to a more complete understanding of the resulting molecular pattern in the future.…”

Section: Discussionmentioning

confidence: 99%

“…A one step extraction/methylation method using trimethylsulphonium hydroxide (TMSH) [9][10][11] as agent for methylation and protection from further metabolic activity in the sample was used as follows: Ten milligrams of frozen tissue were ground with 5 mm stainless steel balls using liquid nitrogen cooling (MM301 Mixer Mill,RetschH, Haan, Germany) for 30 seconds to a fine powder. For blank samples, all steps were performed identically in parallel to the patient samples, but without tissue.…”

Section: Sample Preparation and Derivatisation For Metabolite Analysismentioning

confidence: 99%

Metabolic signature of electrosurgical liver dissection

Schönfels¹,

Kampen²,

Patsenker³

et al. 2013

Z Gastroenterol

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Validation of a one-step extraction/methylation method for determination of fatty acids and cholesterol in marine tissues

Cited by 144 publications

References 42 publications

Three-way Interaction between 14-3-3 Proteins, the N-terminal Region of Tyrosine Hydroxylase, and Negatively Charged Membranes

Three-way Interaction between 14-3-3 Proteins, the N-terminal Region of Tyrosine Hydroxylase, and Negatively Charged Membranes

Use of fatty acid profiles to monitor the escape history of farmed Atlantic salmon

Metabolic signature of electrosurgical liver dissection

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