Validation of a multi-analyte HPLC-DAD method for determination of uric acid, creatinine, homovanillic acid, niacinamide, hippuric acid, indole-3-acetic acid and 2-methylhippuric acid in human urine

Remane, Daniela; Grunwald, Soeren; Hoeke, Henrike; Mueller, Andrea; Roeder, Stefan; Bergen, Martin von; Wissenbach, Dirk K.

doi:10.1016/j.jchromb.2015.06.021

Cited by 30 publications

(20 citation statements)

References 27 publications

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“…A similar observation also reported that the binding capacity of a cryogel towards template human serum albumin (HSA) present in non‐diluted serum is 390.2 mg/g whereas the binding capacity in binding studies is 25.9 mg/g , and also in our previous work reported, MIP towards HSA showed maximum adsorption capacity in urine (129.5 mg/g) whereas the binding capacity in binding studies is 86.7 mg/g . The UA , creatinine , and HSA in the human urine are found to be as ∼0.63–13.6 mM, ∼2.12–17.1 mM, and ∼0.2–0.39 µM, respectively. However, the adsorption capacity of human serum albumin (IF, 1.05) as well as creatinine (IF, 1.21) and uric acid (IF, 1.15), which are final metabolites in urine, is not very significant compared to ATP adsorption efficiency, suggesting that the interference of other metabolites to these ATP binding sites is nonspecific.…”

Section: Resultssupporting

confidence: 76%

“…The adsorption capacity of MIP towards other than adenine base analogues UD, UA, Cre which are also final metabolites in urine, is very low compared to adenine base as well as ADP and AMP both which have the similar structure confirmation, as shown in Supporting Information Figure S11. In general, ADP and AMP concentration in urine is significantly less in comparison to UD, UA, and Cre [53,54]. Even though the adsorption capacity of ADP and AMP was higher than the UD, UA, and Cre, it was attributed to the size and shapes of the imprinting sites are matched with the ADP and AMP which are structurally similar to the template ATP, showing the highest adsorption capacity.…”

Section: Selective Binding Study Of Adenosine 5ʹ-triphosphate Imprintmentioning

confidence: 93%

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Novel adsorptive materials by adenosine 5ʹ‐triphosphate imprinted‐polymer over the surface of polystyrene nanospheres for selective separation of adenosine 5ʹ‐triphosphate biomarker from urine

Jadda

Madhumanchi

Suedee

2019

J of Separation Science

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In this study, we have developed a method to assess adenosine 5ʹ‐triphosphate by adsorptive extraction using surface adenosine 5′‐triphosphate‐imprinted polymer over polystyrene nanoparticles (412 ± 16 nm) for selective recognition/separation from urine. Molecularly imprinted polymer was synthesized by emulsion copolymerization reaction using adenosine 5′‐triphosphate as a template, functional monomers (methacrylic acid, N‐isopropyl acrylamide, and dimethylamino ethylmethacrylate) and a crosslinker, methylenebisacrylamide. The binding capacities of imprinted and non‐imprinted polymers were measured using high‐performance liquid chromatography with UV detection with a detection limit of 1.6 ± 0.02 µM of adenosine 5′‐triphosphate in the urine. High binding affinity (QMIP, 42.65 µmol/g), and high selectivity and specificity to adenosine 5′‐triphosphate compared to other competitive nucleotides including adenosine 5ʹ‐diphosphate, adenosine 5ʹ‐monophosphate, and analogs such as adenosine, adenine, uridine, uric acid, and creatinine were observed. The imprinting efficiency of imprinted polymer is 2.11 for urine (QMIP, 100.3 µmol/g) and 2.51 for synthetic urine (QMIP, 48.5 µmol/g). The extraction protocol was successfully applied to the direct extraction of adenosine 5′‐triphosphate from spiked human urine indicating that this synthesized molecularly imprinted polymer allowed adenosine 5′‐triphosphate to be preconcentrated while simultaneously interfering compounds were removed from the matrix. These submicron imprinted polymers over nano polystyrene spheres have a potential in the pharmaceutical industries and clinical analysis applications.

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Section: Resultssupporting

confidence: 76%

Section: Selective Binding Study Of Adenosine 5ʹ-triphosphate Imprintmentioning

confidence: 93%

Novel adsorptive materials by adenosine 5ʹ‐triphosphate imprinted‐polymer over the surface of polystyrene nanospheres for selective separation of adenosine 5ʹ‐triphosphate biomarker from urine

Jadda

Madhumanchi

Suedee

2019

J of Separation Science

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“…In this test, alkaline picrate reacts with creatinine to form an orange-red product that is measured spectrophotometrically. Other current methods include HPLC-MS/MS [2], HPLC-DAD [3,4], spectrophotometry [5], spectrophotometry using a portable microfluidic system [6], and electrophoresis with electrochemical [7], UV [8], and colorimetric detection [9].…”

Section: Introductionmentioning

confidence: 99%

“…Analytical procedures for the measurement of uric acid include HPLC-DAD [3,4], electrophoresis with UV detection [8], spectrophotometry with chemometric tools [12], electrochemical methods [13], colorimetric detection with multiple indicators [14], enzymatic reaction [15], and gold nanoparticles [16]. Only methods that involve separation techniques, such as chromatography [3,4] and electrophoresis [8], are able to determine both analytes simultaneously. However, these techniques involve the use of large volumes of toxic organic solvents, produce substantial amounts of waste that may be dangerous to the operator and the environment [17], and require laborious clean-up steps.…”

Section: Introductionmentioning

confidence: 99%

Simultaneous determination of renal function biomarkers in urine using a validated paper-based microfluidic analytical device

Rossini

Milani

Carrilho

et al. 2018

Analytica Chimica Acta

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In this paper, we describe a validated paper-based microfluidic analytical device for the simultaneous quantification of two important biomarkers of renal function in urine. This paper platform provides an inexpensive, simple, and easy to use colorimetric method for the quantification of creatinine (CRN) and uric acid (UA) in urine samples. The microfluidic paper-based analytical device (μPAD) consists of a main channel with three identical arms, each containing a circular testing zone and a circular uptake zone. Creatinine detection is based on the Jaffé reaction, in which CRN reacts with picrate to form an orange-red product. Uric acid quantification is based on the reduction of Fe to Fe by UA, which is detected in a colorimetric reaction using 1,10-phenanthroline. Under optimum conditions, obtained through chemometrics, the concentrations of the analytes showed good linear correlations with the effective intensities, and the method presented satisfactory repeatability. The limits of detection and the linear ranges, respectively, were 15.7 mg L and 50-600 mg L for CRN and 16.5 mg L and 50-500 mg L for UA. There were no statistically significant differences between the results obtained using the μPAD and a chromatographic comparative method (Student's t-test at 95% confidence level).

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“…Other method is high performance liquid chromatography with UV absorbance detection [8]. However, the use of the electrochemical methods provides some advantages, such as short analysis time, simple experimental procedures, relatively economical instrumental requirements and high selectivity and sensitivity [2].…”

Section: Introductionmentioning

confidence: 99%

Flavin mononucleotide-exfoliated graphene flakes as electrodes for the electrochemical determination of uric acid in the presence of ascorbic acid

Abellán-Llobregat

Ayán-Varela

Vidal

et al. 2016

Journal of Electroanalytical Chemistry

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Gold interdigitated microelectrodes (Au-IDA) modified with either graphene flakes exfoliated using flavin mononucleotide (FMN) (Gr-FMN) or graphene flakes and platinum nanoparticles (Pt-Gr-FMN) have been studied in the oxidation of uric acid (UA). An electrochemical method for the detection and quantification of UA in phosphate buffer solution at physiological pH (PBS, 0.25 M, pH 7) in the absence and presence of ascorbic acid (AA) has been studied by cyclic voltammetry.The quantification of UA was investigated by cyclic voltammetry, presenting an oxidation peak at 0.99 V with both modified electrodes. Linearity range of 60-578 µM and 60-345 µM has been found for Gr-FMN/Au-IDA and Pt-Gr-FMN/Au-IDA electrodes, respectively. Limits of detection of 18 µM were obtained for both electrodes, and the repeatability was studied at 177 µM providing 4% and 8% for Gr-FMN/Au-IDA and Pt-Gr-FMN/Au-IDA, respectively. AA interference has been studied by cyclic voltammetry, showing two clearly separated oxidation peaks, at 0.99 V for UA oxidation and at 0.74 V for Gr-FMN/Au-IDA and 0.70 V for Pt-Gr-FMN/Au-IDA for AA oxidation. Linearity range has been studied in presence of 250 µM AA obtaining a working range of 60-578 µM for Gr-FMN/Au-IDA electrode and of 60-288 µM with Pt-Gr-FMN/Au-IDA electrode.Limits of detection remain at 18 µM for both electrodes and the repeatability was studied at 177 µM providing 8% and 14% for Gr-FMN/Au-IDA and Pt-Gr-FMN/Au-IDA electrodes respectively.

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Validation of a multi-analyte HPLC-DAD method for determination of uric acid, creatinine, homovanillic acid, niacinamide, hippuric acid, indole-3-acetic acid and 2-methylhippuric acid in human urine

Cited by 30 publications

References 27 publications

Novel adsorptive materials by adenosine 5ʹ‐triphosphate imprinted‐polymer over the surface of polystyrene nanospheres for selective separation of adenosine 5ʹ‐triphosphate biomarker from urine

Novel adsorptive materials by adenosine 5ʹ‐triphosphate imprinted‐polymer over the surface of polystyrene nanospheres for selective separation of adenosine 5ʹ‐triphosphate biomarker from urine

Simultaneous determination of renal function biomarkers in urine using a validated paper-based microfluidic analytical device

Flavin mononucleotide-exfoliated graphene flakes as electrodes for the electrochemical determination of uric acid in the presence of ascorbic acid

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