Validation of a low-cost, liquid-based screening method for cervical intraepithelial neoplasia

Lee, Judy M.; Kelly, Deidra; Gravitt, Patti E.; Fansler, Zoya; Maksem, John A.; Clark, Douglas P.

doi:10.1016/j.ajog.2006.02.001

Cited by 17 publications

(28 citation statements)

References 18 publications

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“…The polymer solution used in our laboratory contains agarose, polyethyelene glycol, alcohol and poly -L-Lysine which help to form a cell button and form a thin monolayer of cells within a clean background (Jonhson et al, 2000;Maksem et al, 2001;Kavatkar et al, 2008). Thus by giving a clear background and removal of contaminating mucus & blood, MLBC improves the quality of screening of slides (Austin and Ramzy, 1998;Maksem et al, 2001;Lee, 2006;Deshou et al, 2009).…”

Section: Discussionmentioning

confidence: 99%

“…The thin layer preparation cells functions as part of the enrichment process by reducing unreadable, thick areas where cells of interest can hide (Baandrup et al, 2000). Although LBC is recommended for cervical cancer screening, it involves the use of automated devices which are high cost per test (Maksem et al, 2001;Lee et al, 2006;Kavatkar et al, 2008;Deshou et al, 2009).…”

Section: Discussionmentioning

confidence: 99%

“…Preparation (Richard et al, 1999) and detection of HPV DNA by PCR or in situ DNA hybridization (Maksem et al, 2001;Lee et al, 2006;Kavatkar et al, 2008;Deshou et al, 2009). …”

Section: Discussionmentioning

confidence: 99%

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Manual Liquid Based Cytology in Primary Screening for Cervical Cancer - a Cost Effective Preposition for Scarce Resource Settings

Nandini

Nandish²,

Pallavi³

et al. 2012

Asian Pacific Journal of Cancer Prevention

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Conventional pap smear (CPS) examination has been the mainstay for early detection of cervical cancer. However, its widespread use has not been possible due to the inherent limitations, like presence of obscuring blood and inflammation, reducing its sensitivity considerably. Automated methods in use in developed countries may not be affordable in the developing countries due to paucity of resources. On the other hand, manual liquid based cytology (MLBC) is a technique that is cost effective and improves detection of precursor lesions and specimen adequacy. Therefore the aim of the study was to compare the utility of MLBC with that of CPS in cervical cancer screening. A prospective study of 100 cases through MLBC and CPS was conducted from October 2009 to July 2010, in a Medical College in India, by two independent pathologists and correlated with histopathology (22 cases). Morphological features as seen through MLBC and CPS were compared. Subsequently, all the cases were grouped based on cytological diagnosis according to two methods into 10 groups and a subjective comparison was made. In order to compare the validity of MLBC with CPS in case of major diagnoses, sensitivity and specificity of the two methods were estimated considering histological examination as the gold standard. Increased detection rate with MLBC was 150%. The concordance rate by LBC/histopathology v/s CPS/histopathology was also improved (86% vs 77%) The percentage agreement by the two methods was 68%. MLBC was more sensitive in diagnosis of LSIL and more specific in the diagnosis of inflammation. Thus, MLBC was found to be better than CPS in diagnosis of precursor lesions. It provided better morphology with increased detection of abnormalities and preservation of specimen for cell block and ancillary studies like immunocytochemistry and HPV detection. Therefore, it can be used as alternative strategy for cervical cancer prevention in limited resource settings

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Manual Liquid Based Cytology in Primary Screening for Cervical Cancer - a Cost Effective Preposition for Scarce Resource Settings

Nandini

Nandish²,

Pallavi³

et al. 2012

Asian Pacific Journal of Cancer Prevention

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“…In most of these reports, a significant rise in sensitivity was achieved with liquid-based procedures, without major losses in specificity. 4,5 Cell blocks prepared from residual tissue fluids and fineneedle aspirations can be useful adjuncts to smears for establishing a more definitive cytopathological diagnosis and they can be a valuable diagnostic adjunct in cytological preparation in selected fluid-based cervicovaginal specimens. 6 Objectives of the Study The Aims and Objectives of the Study are-1.…”

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confidence: 99%

Advantages of Manual Liquid-Based Cytology in Cervicovaginal Smears With Special Emphasis on Cell Block

Kulkarni¹,

Manoli²,

Manoli³

2017

jemds

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“…One of these alternative monolayer slide preparation methods makes use of a cytocentrifuge and an alcoholic-agar solution, named 3MLBC. [5][6][7] Other known systems in Europe using the Hettich cytocentrifuge are CytoSCREEN 1 (Seroa, Monaco, France) and Turbitec 1 (Labonord, Templemars, France). In this quality control study, we determined the sensitivity and specificity of LBC with the Turbitec cytocentrifuge technique in a series of 632 women who recently had an abnormal cervical smear and were referred to our CIN outpatient clinic.…”

mentioning

confidence: 99%

Accuracy of a low priced liquid‐based method for cervical cytology in 632 women referred for colposcopy after a positive Pap smear

Hemel

Buikema

Groen

et al. 2009

Diagnostic Cytopathology

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The aim of this quality controlling study was to determine the accuracy of liquid‐based cytology (LBC) with the Turbitec® cytocentrifuge technique. Cervical smears of 632 women, who were referred to our CIN outpatient department, after at least two smears with ASCUS or higher were evaluated and compared with the histological outcome. In 592 cases the smears revealed abnormalities of squamous epithelium, and in 40 cases the abnormalities of glandular epithelium. In the group of squamous epithelium abnormalities, the sensitivity for LSIL was 39.7% and the specificity was 89.2%; for the LSIL+ group, these values were 89.4% and 91.4%, respectively. For HSIL the sensitivity was 68.3% and the specificity 92.8%, for the HSIL+ group 82.3% and 92.3%, respectively. The ASCUS rate was low (2.4%). The Turbitec cytocentrifuge method was proved to be a very good LBC method for cervical smears. Because of a comparable accuracy together with a lower price, this LBC method outweighs commercial alternatives. Diagn. Cytopathol. 2009. © 2009 Wiley‐Liss, Inc.

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Validation of a low-cost, liquid-based screening method for cervical intraepithelial neoplasia

Cited by 17 publications

References 18 publications

Manual Liquid Based Cytology in Primary Screening for Cervical Cancer - a Cost Effective Preposition for Scarce Resource Settings

Manual Liquid Based Cytology in Primary Screening for Cervical Cancer - a Cost Effective Preposition for Scarce Resource Settings

Advantages of Manual Liquid-Based Cytology in Cervicovaginal Smears With Special Emphasis on Cell Block

Accuracy of a low priced liquid‐based method for cervical cytology in 632 women referred for colposcopy after a positive Pap smear

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