Validation of a human progesterone enzyme immunoassay (EIA) kit for use on serum of cattle in Cameroon

Bayemi, P. H.; Nsongka, V. M.; Perera, B.M.A.O.; Cavestany, D.; Webb, E.C.

doi:10.1007/s11250-007-9020-6

Cited by 10 publications

(7 citation statements)

References 3 publications

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“…Results showed that both of the ELISA KIT used are capable and have a good validity for measuring both hormones. Other validation tests were also been reported by other researchers to measure progesterone in cattle (Bayemi et al, 2007), and thyroid hormone in donkey (Todini et al, 2010).…”

Section: Biological Validationmentioning

confidence: 87%

“…The mean percentage of accuracy for this assay was 99.65±4.27% whereas, intra-and inter-assay coefficients of variation (% CV) of high and low value quality controls for the assay precision were below 10% and 15% respectively. Those results indicating that the accuracy and precision of assay were acceptable for measuring serum testosterone in this species (Bayemi et al, 2007;Heisterman, 2010;Bielohuby et al, 2012). Both accuracy and precision reflect how close a measurement is to the actual value.…”

Section: Analytical Validationmentioning

confidence: 91%

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MEASUREMENT OF SERUM TESTOSTERONE IN KACANG GOAT BYUSING ENZYME-LINKED IMMUNOSORBENT ASSAY (ELISA) TECHNIQUE: THE IMPORTANCE OF KIT VALIDATION (Pengukuran Testosteron Serum Kambing Kacang dengan Teknik Enzyme-Linked Immunosorbent Assay (ELISA): Pentingnya Validasi Kit)

G¹,

Wahyuni²,

kadar³

et al. 2016

J Kedokt Hewan

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This study was conducted to validate a commercial testosterone enzyme-linked immunosorbent assay (ELISA) kits (DRG EIA-1559) inanalytic and biological manner for measuring serum testosterone concentrations in kacang goats. This study used 18 healthy kacang goats, sixbucks (>2 years), six kids (<6 months), and six does (>2 years). Blood samples were collected from jugular vein and prepared as serum. Twovalidation tests were performed, an analytical validation comprises a parallelism, accuracy, precision and sensitivity and a biological validationby comparing testosterone concentration from bucks, kids, and does. Testosterone concentrations were measured using ELISA technique. Data ofanalytical validation were analyzed descriptively and test of equality of slope was performed to see the parallelism between samples and standardcurves. Analysis of variance (ANOVA) was used for biological validation data. Results of parallelism showed that sample curve was parallel tothe standard curve. Accuracy, precision (% CV of intra-and inter-assay) and sensitivity of the assay were 99.65±4.27%, <10%, <15% and 0.083ng/ml, respectively. Results of biological validation showed that the assay used were accurately measured testosterone which testosteroneconcentrations in bucks were significantly higher compared to kids and does (P<0.05). In conclusion, a commercial testosterone ELISA kits(DRG EIA-1559) is a reliable assay for measuring serum testosterone concentration in kacang goats. Key words: analytical and biological validations, ELISA, testosterone, kacang goat

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Section: Biological Validationmentioning

confidence: 87%

Section: Analytical Validationmentioning

confidence: 91%

MEASUREMENT OF SERUM TESTOSTERONE IN KACANG GOAT BYUSING ENZYME-LINKED IMMUNOSORBENT ASSAY (ELISA) TECHNIQUE: THE IMPORTANCE OF KIT VALIDATION (Pengukuran Testosteron Serum Kambing Kacang dengan Teknik Enzyme-Linked Immunosorbent Assay (ELISA): Pentingnya Validasi Kit)

G¹,

Wahyuni²,

kadar³

et al. 2016

J Kedokt Hewan

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“…It is an important reproductive hormone to maintain pregnancy and is produced by the corpus luteum of the ovary. Upon measuring, its blood level concentration increases after ovulation and during pregnancy and reduces during follicular growth after corpus luteum regress and parturition [ 12 ]. Progesterone concentration is maintained by corpus luteum at a high level in pregnant cows compared with non-pregnant cows [ 5 ].…”

Section: Discussionmentioning

confidence: 99%

“…The human progesterone ELISA kit was able to make the difference between the PG and NPG cattle; in that, the concentration of progesterone in NPG cows was less than 1.2 ng/ml, while it was above 3 ng/ml for the PG cows. Different experiments for veterinary progesterone kits assert similar distinctions between PG and NPG animals [ 12 ].…”

Section: Discussionmentioning

confidence: 99%

Validation of the Human Progesterone Assay Kit for Cattle as a Pregnancy Diagnosis Tool

Lobago

Lemma

et al. 2022

Veterinary Medicine International

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Accurate pregnancy diagnosis is an important criterion and management tool for successful dairying. Early identification of non-pregnant dairy heifers and cows after breeding can improve pregnancy rate and life time production. Determination of progesterone hormone levels is more accurate to diagnose failed pregnancies in dairy animals. This method is not always available in developing countries. Some of the kits available are developed for humans and might be used for cattle because in principle, progesterone is not species-specific and detection methods are the same in both animals and human beings. The study aimed at validating a human progesterone ELISA kit for use in cattle as a pregnancy diagnosis tool. Forty Boran and crossbred cattle (22 pregnant and 18 non-pregnant) were selected for the study. Ten milliliter of blood sample was collected from each animal using jugular venipuncture. Serum I and plasma was harvested within 2 hours after venipuncture and serum II after 12 hours, and all samples were analyzed for progesterone concentration using the ELISA procedure provided with the kit. The result showed that 88.9% (n = 16) of non-pregnant cows had progesterone concentration below 1 ng/ml with mean (±SE) of 0.48 ± 0.75 ng/ml while all pregnant cows had mean (±SE) concentration of 19.3 ± 0.68 ng/ml with individual values ranging from 5.2–38 ng/ml. Progesterone concentration between breeds and sample type did not show statistically significant difference for pregnant and non-pregnant cows. Nonetheless, the results of the experiments are very promising as far as pregnancy diagnosis is concerned in dairy cows from an economic perspective and accuracy; the experiments have to be performed on larger scale to proof repeatability and sensitivity

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“…Cows with 1 ng/ml for two consecutive samples or one sample at or above 3 ng/ml are an indication of the presence of corpus luteum while cows below 1 ng/ml will be in anoestrus. Therefore the kit was used for monitoring post-partum progesterone profiles in dairy herds (Bayemi et al 2007a). Pregnant dairy cows were selected for studies on post partum return to ovarian activity and milk production.…”

Section: Reproductionmentioning

confidence: 99%

An integrated method for improving the dairy production sector in developing countries: the case of Cameroon

Bayemi

Webb

2008

Trop Anim Health Prod

Self Cite

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During the past six years, ten research topics were carried out with the aim of developing an integrated method to improve production and sustainability of dairy systems in Cameroon. This involved reviewing dairy research done in the country, carrying out a participatory rural appraisal and an economic opportunity survey in selected dairy farms, setting up on-farm interventions, investigating cow reproduction, evaluating milk quality and the impact of integrated interventions. Guidelines for improvement of the dairy sector were set up. It was found that the developed integrated method had a positive impact on dairy farms. Farmers who adopted interventions had nearly 200% higher economic returns. In order to boost the Cameroonian dairy sector, it is suggested that the government acts as a motivating force by organizing the market, ensuring the monitoring of epizootic diseases and providing artificial insemination services and organizing breeding societies. It is also suggested that the integrated method becomes a discipline in dairy science and be applied in other developing countries.

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Validation of a human progesterone enzyme immunoassay (EIA) kit for use on serum of cattle in Cameroon

Cited by 10 publications

References 3 publications

MEASUREMENT OF SERUM TESTOSTERONE IN KACANG GOAT BYUSING ENZYME-LINKED IMMUNOSORBENT ASSAY (ELISA) TECHNIQUE: THE IMPORTANCE OF KIT VALIDATION (Pengukuran Testosteron Serum Kambing Kacang dengan Teknik Enzyme-Linked Immunosorbent Assay (ELISA): Pentingnya Validasi Kit)

MEASUREMENT OF SERUM TESTOSTERONE IN KACANG GOAT BYUSING ENZYME-LINKED IMMUNOSORBENT ASSAY (ELISA) TECHNIQUE: THE IMPORTANCE OF KIT VALIDATION (Pengukuran Testosteron Serum Kambing Kacang dengan Teknik Enzyme-Linked Immunosorbent Assay (ELISA): Pentingnya Validasi Kit)

Validation of the Human Progesterone Assay Kit for Cattle as a Pregnancy Diagnosis Tool

An integrated method for improving the dairy production sector in developing countries: the case of Cameroon

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