Validation of a HPLC-DAD method for quantification of ascorbic acid in mixed juice powder of acerola and seriguela / Validação de um método HPLC-DAD para quantificação de ácido ascórbico de suco misto em pó de acerola e seriguela

Abstract

The objective of this work was to optimize extraction process and to validate the HPLC-DAD method for quantification of ascorbic acid content in mixed juice powder of acerola and seriguela with different dextrose equivalent maltodextrin (5, 10, 15) as an encapsulating agent. The influence of the extraction variables was studied through factorial design. Control variables were mass, percent of orthophosphoric acid and stirring time. Chromatographic method was validated in terms of precision, selectivity, linearity and accuracy. Chromatographic and titrimetry results were compared for all dextrose equivalent. Mass and percentage of orthophosphoric acid presented p 0.05. There were no significant differences among the dextrose equivalents ranging from 2,830.1 mg/100 g to 2,931.61 mg/100 g of ascorbic acid. Both values, intra and intermediary precision test coefficients of variation were less than 5%. The correlation coefficient was 0.99. Respective value of limit of detection was 0.001 mg/mL and for quantification was 0.003 mg/mL. The application of HPLC-DAD method can be considered acceptable for quantification ascorbic acid in fruit powder.

Preparation, Characterization, and Antioxidant Activity of L-Ascorbic Acid/HP-β-Cyclodextrin Inclusion Complex-Incorporated Electrospun Nanofibers

“…A viscometer (IKA ® ROTAVISC LO-VI S000, Staufen, Germany) was used to measure the viscosity of the LAA/HP- β -CD solution, at 23 ± 1 °C. The entrapment efficiency of LAA/HP- β -CD-NF was assessed by high-performance liquid chromatography (HPLC), as per the method described by Reibero et al, with minor modifications [ 31 ]. The quantitative analysis of LAA was performed utilizing an HPLC system (Agilent 1260 Infinity II, Waldbronn, Germany) equipped with a quaternary pump (G7111A, 1260 Quat pump VL), an automatic vial sampler injector (G7129A, 1260 Vialsampler), a multicolumn thermostat (G7116A, 1260 MCT), and a DAD detector (G7115A, 1260 DADWR).…”