Validation of a hematology analyzer in donkey medicine

Ferreira, A.M.; Meneses, G.; Queiroga, F.L.; Martins, A.; Cruz, Z.; Silvestre-Ferreira, A.C.

doi:10.1016/j.jevs.2023.104984

Journal of Equine Veterinary Science

2024

DOI: 10.1016/j.jevs.2023.104984

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Validation of a hematology analyzer in donkey medicine

A.M. Ferreira,

G. Meneses,

F.L. Queiroga

et al.

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2024

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Cited by 2 publications

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Coagulation Profile of the Healthy Miranda’s Donkey

Silva,

Queiroga,

Cruz

et al. 2024

Animals

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Miranda’s donkey, originating in northern Portugal, is an autochthonous breed that is deeply intertwined with the region’s culture and economy. Knowledge of the physiological characteristics of the breed is important for its preservation, and several studies have been carried out, but none have focused on its coagulation profile. The aim of this study was to establish reference intervals (RIs) for coagulation in healthy Miranda’s donkey and to assess the influence of sex and age. Blood samples from 75 clinically healthy animals were analyzed for seven coagulation parameters: four using IDEXX ProCyte Dx and three using Start® 4-Diagnostica-Stago. The RI values were calculated following the ASVCP guidelines and with the Reference Advisor V.2.1 software. To analyze the influence of sex and age, SPSS version 29 was used. No significant differences were found between sexes (p > 0.05), but statistically significant differences were found between ages (p < 0.05) for platelet count and plateletcrit (both higher in young animals). The RIs described here can help monitor health and guide the diagnosis and treatment of diseased Miranda’s donkeys, contributing to their preservation. Our study encourages further research on coagulation in donkeys and the use of different methodologies to obtain information for veterinarians working with this species.

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Coagulation Profile of the Healthy Miranda’s Donkey

Silva,

Queiroga,

Cruz

et al. 2024

Animals

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Analytical Validation of Two Point-of-Care Assays for Hematological Analysis in the Miranda Donkey

Costa,

Sousa,

Silva

et al. 2024

Veterinary Sciences

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Miranda donkeys are an endangered autochthonous breed of Portugal. The HemoCue WBC DIFF and HemoCue Hb 201 portable analyzers, developed as a simplified alternative method for total and differential WBC count and hemoglobin measurement, respectively, may be relevant tools in veterinary practice. This study aimed to validate these instruments using Miranda donkey blood samples. For the HemoCue WBC DIFF, most parameters showed acceptable intra- and inter-assay precision with coefficients of variation (CV) below 5%, except for monocytes and eosinophils with higher CVs. The HemoCue Hb 201 showed CVs of 1.98% and 4.07%. Linearity correlation coefficients (r) ranged from 0.53 to 0.99 for HemoCue WBC DIFF and 0.99 for HemoCue Hb 201. Significant levels for neutrophils, lymphocytes, monocytes, eosinophils, and Hb measurements varied. Comparisons with ProCyte Dx showed an excellent correlation for WBC (rs = 0.96), neutrophils (rs = 0.91), lymphocytes (rs = 0.94), and eosinophils (rs = 0.90) but a poor correlation for monocytes and basophils. The HemoCue Hb 201 showed a correlation of rs = 0.96 with ProCyte Dx. In conclusion, both analyzers provided reliable results and are suitable for use in Miranda’s donkey breed for WBC counts and Hb measurements.

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Validation of a hematology analyzer in donkey medicine

Cited by 2 publications

References 20 publications

Coagulation Profile of the Healthy Miranda’s Donkey

Coagulation Profile of the Healthy Miranda’s Donkey

Analytical Validation of Two Point-of-Care Assays for Hematological Analysis in the Miranda Donkey

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