Validation of a fast gas chromatographic method for the study of semiochemical slow release formulations

Heuskin, Stéphanie; Rozet, Eric; Lorge, Stéphanie; Farmakidis, Julien; Hubert, Philippe; Verheggen, François; Haubruge, Éric; Wathelet, Jean‐Paul; Lognay, Georges

doi:10.1016/j.jpba.2010.07.014

Cited by 17 publications

(16 citation statements)

References 42 publications

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“…For quantitative analyses (percentage determination), we used fast GC, which proved to be powerful for analysis of essential oil constituents (Bicchi et al. 2004; Heuskin et al. 2009, 2010).…”

Section: Methodsmentioning

confidence: 99%

Effective concentrations of garlic distillate (Allium sativum) for the control of Tetranychus urticae (Tetranychidae)

Attia

Grissa

Mailleux

et al. 2011

J Applied Entomology

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The two‐spotted spider mite, Tetranychus urticae Koch, is a worldwide pest that feeds on a large variety of plant families. Because its resistance to acaricides is spreading rapidly, the development of new biological control tactics for population management is crucial. Plant extracts, such as garlic extract (Allium sativum Linn.), may represent viable alternatives, because they are currently considered to be minimum‐risk pesticides. Although garlic is known for its acaricidal properties, the extract concentration that provides the most efficient control has not yet been precisely determined. In this study, we conducted a series of laboratory experiments to determine the susceptibility of adult females to different concentrations of garlic extract. Fresh garlic cloves were steam‐distilled and sprayed using a Potter spray tower. Mortality and fecundity were measured upon treatment with garlic extract concentrations ranging from 0.46 to 14.4 mg/l. Female mortality increased with concentration, with LD50 and LD90 values of 7.49 and 13.5 mg/l, respectively. Reduced fecundity was previously observed at concentrations of 0.36 and 0.74 mg/l. The chemical composition of the Allium sativum distillate was characterized by reversed‐phase high‐performance liquid chromatography coupled with photodiode array detection, GC/MS and Fast GC‐FID against an authentic standard (Standard, Bioextract).Vinyl dithiin, diallyl disulphide, diallyl trisulphide and methyl allyl trisulphide were identified based on their mass spectra. Sesquiterpenoids were identified by their retention index.

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Section: Methodsmentioning

confidence: 99%

Effective concentrations of garlic distillate (Allium sativum) for the control of Tetranychus urticae (Tetranychidae)

Attia

Grissa

Mailleux

et al. 2011

J Applied Entomology

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“…Sesquiterpene analyses and quantification of volatile collection extracts by fast GC were validated according to the accuracy profile procedure described in Heuskin et al (2010) with longifolene as internal standard for quantification. The chromatographic conditions are the same than the one previously detailed in previous papers (Heuskin et al, 2009(Heuskin et al, , 2010(Heuskin et al, , 2012.…”

Section: Fast Gc Analysesmentioning

confidence: 99%

“…The chromatographic conditions are the same than the one previously detailed in previous papers (Heuskin et al, 2009(Heuskin et al, , 2010(Heuskin et al, , 2012.…”

Section: Fast Gc Analysesmentioning

confidence: 99%

“…This polysaccharide having low oxygen permeability can protect the sesquiterpenes from oxidation. The protection efficiency of alginate beads towards both molecules was demonstrated in Heuskin et al (2010). This formulation was optimised in terms of semiochemical encapsulation capacity and already proved its biological efficiency by attracting aphid parasitoids, Aphidius ervi (Heuskin et al, 2012).…”

Section: Introductionmentioning

confidence: 99%

“…First of all, volatile organic compounds were extracted and purified from essential oils of Matricaria chamomilla L. (Asteraceae) and Nepeta cataria L. (Lamiaceae) for E--farnesene and E--caryophyllene, respectively (Heuskin et al, 2009(Heuskin et al, , 2010. Secondly, alginate biodegradable matrix was chosen to formulate semiochemicals.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

A Semiochemical Slow-release Formulation in a Biological Control Approach to Attract Hoverflies

Heuskin

Lorge

Leroy

et al. 2012

JEE

Self Cite

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E-β-Farnesene, the alarm pheromone of many aphid species, and E-β-caryophyllene are considered as two sesquiterpenes attractive for aphid predators, among which Syrphidae species. Both compounds were formulated in alginate gel beads as slow-release devices in a biological control approach against aphids. Semiochemical diffusion from beads was studied in the laboratory according to abiotic parameters. Efficiency of formulations as hoverfly attractant was demonstrated in field experiments from June to August 2009. The diffusion of semiochemicals from alginate bead formulations was principally limited by high values (>85%) of relative humidity in the air. Temperature also impacts the release of volatile compounds. In field experiments, these two abiotic factors were supposed to highly condition the trapping of hoverflies. These field trappings demonstrated the efficiency of both semiochemical formulations compared to control (formulation without semiochemical) to catch females of Syrphidae during three months.This research proved the efficiency of semiochemical alginate formulations as potential biological control tool to attract aphid predators.

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Flexibility and applicability of β‐expectation tolerance interval approach to assess the fitness of purpose of pharmaceutical analytical methods

Bouabidi

Talbi

Bourichi

et al. 2012

Drug Testing and Analysis

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An innovative versatile strategy using Total Error has been proposed to decide about the method's validity that controls the risk of accepting an unsuitable assay together with the ability to predict the reliability of future results. This strategy is based on the simultaneous combination of systematic (bias) and random (imprecision) error of analytical methods. Using validation standards, both types of error are combined through the use of a prediction interval or β-expectation tolerance interval. Finally, an accuracy profile is built by connecting, on one hand all the upper tolerance limits, and on the other hand all the lower tolerance limits. This profile combined with pre-specified acceptance limits allows the evaluation of the validity of any quantitative analytical method and thus their fitness for their intended purpose. In this work, the approach of accuracy profile was evaluated on several types of analytical methods encountered in the pharmaceutical industrial field and also covering different pharmaceutical matrices. The four studied examples depicted the flexibility and applicability of this approach for different matrices ranging from tablets to syrups, different techniques such as liquid chromatography, or UV spectrophotometry, and for different categories of assays commonly encountered in the pharmaceutical industry i.e. content assays, dissolution assays, and quantitative impurity assays. The accuracy profile approach assesses the fitness of purpose of these methods for their future routine application. It also allows the selection of the most suitable calibration curve, the adequate evaluation of a potential matrix effect and propose efficient solution and the correct definition of the limits of quantification of the studied analytical procedures.

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Validation of a fast gas chromatographic method for the study of semiochemical slow release formulations

Cited by 17 publications

References 42 publications

Effective concentrations of garlic distillate (Allium sativum) for the control of Tetranychus urticae (Tetranychidae)

Effective concentrations of garlic distillate (Allium sativum) for the control of Tetranychus urticae (Tetranychidae)

A Semiochemical Slow-release Formulation in a Biological Control Approach to Attract Hoverflies

Flexibility and applicability of β‐expectation tolerance interval approach to assess the fitness of purpose of pharmaceutical analytical methods

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