Validation of a confirmatory method for the determination of sulphonamides in muscle according to the European Union regulation 2002/657/EC

Pecorelli, Ivan; Bibi, Rita; Fioroni, Laura; Galarini, Roberta

doi:10.1016/j.chroma.2003.11.010

Cited by 73 publications

(45 citation statements)

References 15 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…13 (11,13,19). According to validation data, methods of Papapanagiotou et al (9,10) are suitable for SMZ analysis in chicken meat and liver.…”

Section: Discussionmentioning

confidence: 99%

Effects of various freezing and cooking processes on the residues of sulfamethazine in broiler tissues

KANBUR

2015

Ankara Üniversitesi Veteriner Fakültesi Dergisi

View full text Add to dashboard Cite

Summary:This study was conducted to investigate the effects of frying, boiling and freezing processes on sulfamethazine (SMZ) residues in chick breast meat and liver. Chicks were fed with feed not containing sulfonamide residues for 30 days. After 30 days, the suspensions of SMZ (110 mg/kg) were given to 10 chicken by gavage to craw once daily for 5 days. After 35 day, all animals were slaughtered and their breast meat and liver were taken. The part of the tissues were stored in -20 C freezer for 30 and 45 days. The levels of SMZ in raw, grilled and boiled and freezed tissues were determined by HPLC-DAD detector. As a result of the study, it has been determined that, boiling and grilling processes reduced the residues of SMZ at different rates in broiler tissues; storing in the deep freezer did not cause significant changes on drug residues.Key words: Breast meat, broiler, cooking, freezing, liver, sulfamethazine Sülfametazinin etçi piliç dokularındaki kalıntıları üzerine çeşitli pişirme ve dondurma işlemlerinin etkileriÖzet: Bu araştırmayla kızartma, haşlama ve dondurma işlemlerinin etçi piliç göğüs eti ve karaciğer dokularındaki sülfametazin (SMZ) kalıntılarına yönelik etkilerinin ortaya konulması amaçlandı. Civcivler 30 gün boyunca sülfonamid içermeyen yemle beslendi. Otuzuncu günün sonunda deneme grubuna SMZ (110 mg/kg) günde 1 kez ve 5 gün boyunca sonda ile kursağa verildi. 35. günün sonunda tüm hayvanlar kesilerek sağ göğüs eti ve karaciğerleri alındı. Dokuların bir kısmı 30 ve 45 gün süresince -20 C'lik derin dondurucuda saklandı. Çiğ dokular, kızartma ve haşlama yapılan dokular ile derin dondurucuda muhafaza edilen dokulardaki SMZ düzeyleri HPLC-DAD dedektörle belirlendi. Çalışma sonucunda broyler göğüs eti ve karaciğer dokularındaki SMZ düzeylerinin ızgara ve haşlama işlemleri ile değişik oranlarda azaldığı; dokularda SMZ kalıntıları üzerinde derin dondurucuda muhafaza işleminin önemli bir değişime yol açmadığı belirlendi.

show abstract

“…13 (11,13,19). According to validation data, methods of Papapanagiotou et al (9,10) are suitable for SMZ analysis in chicken meat and liver.…”

Section: Discussionmentioning

confidence: 99%

Effects of various freezing and cooking processes on the residues of sulfamethazine in broiler tissues

KANBUR

2015

Ankara Üniversitesi Veteriner Fakültesi Dergisi

View full text Add to dashboard Cite

show abstract

“…Dentre os diversos estudos disponíveis na literatura para determinação de sulfonamidas em músculo de camarão, a cromatografia líquida de alta eficiência (CLAE) tem sido reportada como técnica analí-tica frequentemente utilizada devido a sua precisão e recuperação satisfatórias. [20][21][22][23] Este trabalho teve como objetivo otimizar e validar um méto-do multirresíduo para determinação de sulfonamidas (sulfatiazol, sulfametazina, sulfadimetoxina e sulfaquinoxalina) em músculo de camarão comercial utilizando cromatografia líquida de alta eficiência com detecção por UV.…”

Section: Figura 1 Estruturas Químicas Das Sulfonamidasunclassified

Otimização e validação de método multirresíduo para determinação de sulfonamidas em camarão cultivado por cromatografia líquida de alta eficiência com detecção por UV

Santos¹,

Maciel²,

Lavorante³

et al. 2011

Quím. Nova

View full text Add to dashboard Cite

Recebido em 8/10/10; aceito em 3/2/11; publicado na web em 15/4/11 OPTIMIZATION AND VALIDATION MULTIRESIDUE METHOD FOR SULFONAMIDES DETERMINATION IN SHRIMP BY HIGH PERFORMANCE LIQUID CHROMATOGRAPHY WITH DETECTION BY UV. This work reports the optimization and method validation for sulfonamides (sulfamethazine, sulfaquinoxaline, sulfadimethoxine and sulfathiazole) in shrimp muscle using HPLC-UV. The sulfonamides were extracted with acetonitrile and acetic acid, and the extract cleaned up with a Strata SCX SPE cartridge prior to analysis. The method presented linearity in the range of 20-120 µg kg . The recovery for shrimp muscles spiked with 50-150 µg kg -1 ranged from 63.2-108.0%. Precision and accuracy analysis showed acceptable relative standard deviation. Commercial shrimps were analyzed and sulfonamides don't were found above of the method limit of quantification.Keywords: sulfonamides; shrimp; HPLC-UV. INTRODUÇÃOUma grande variedade de antimicrobianos está disponível para uso na medicina veterinária, inclusive na aquicultura. Entre esses, destacam-se os antimicrobianos das classes dos b-lactâmicos, sulfonamidas, tetraciclinas, aminoglicosídeos, macrolídeos, quinolonas e anfenicois, que são compostos que inibem o crescimento de determinados micro-organismos, sendo utilizados na produção animal para tratar e prevenir enfermidades causadas pela presença de organismos patogênicos e melhorar a taxa de crescimento ou conversão alimentar. 1Dentre os antimicrobianos sintéticos, as sulfonamidas (SAs) são um importante grupo que tem sido usado efetivamente no combate às infecções bacterianas e também na prática veterinária para promover o crescimento animal (Figura 1).O termo sulfonamida é utilizado para referir-se aos derivados do para-amino-benzeno-sulfonamida (sulfanilamida). O grupo p-NH 2 desses compostos é essencial e só pode ser substituído por radicais capazes de serem convertidos in vivo em grupos amino livre. Essas substituições possuem efeitos variáveis sobre a atividade antibacteriana da molécula. As sulfonamidas são análogos estruturais e antagonistas competitivos do ácido para-aminobenzoico (PABA) e impedem a sua utilização pelas bactérias na síntese do ácido fólico ou vitamina B9. Mais especificamente, as sulfonamidas são inibidores competitivos da di-hidropteroato-sintetase, a enzima bacteriana responsável pela incorporação do PABA no ácido di-hidropteroico, precursor imediato do ácido fólico. Os micro-organismos sensí-veis são aqueles que precisam sintetizar seu próprio ácido fólico, as bactérias capazes de utilizar o folato pré-formado não são afetadas. 2A carcinicultura é uma das áreas mais consolidadas do agronegócio brasileiro, sendo a região Nordeste produtora de mais de 90% de todo o camarão cultivado no Brasil que, em sua maioria, é destinado ao mercado europeu.3 Apesar do crescimento acelerado até 2003, a carcinicultura brasileira enfrentou alguns desafios nos anos seguintes, como a aplicação de taxas pelos países importadores e o surgimento de doenças infecciosas nos animais cultivados, fatos...

show abstract

“…A number of analytical techniques such as colorimetric method, Thin Layer Chromatography, High Performance-Thin Layer Chromatography is used for mycotocins, Gas Liquid Chromatography, Gas Chromatography-Mass Spectrometry, High Performance Liquid Chromatography (HPLC), Liquid Chromatography-Mass Spectrometry-Mass Photometry etc., are established for detection and quantification of pesticide residues in animal tissues [56]. However, colorimetric and Thin Layer Chromatography methods are limited only for qualitative determination due to its low sensitivity.…”

Section: Physico-chemical Assaysmentioning

confidence: 99%

Review on Chemical Residues in Milk and Their Public Health Concern in Ethiopia

Aytenfsu¹,

Mamo²

2016

J Nutr Food Sci

View full text Add to dashboard Cite

Chemical residues which could contaminants cow milk are chlorinated pesticides, organophosphates, herbicides, fungicides, antihelminthetic drugs, antibiotic, hormones, detergents and disinfectants, nitrites, poly chlorinated, poly brominated biphenyles, dioxins, mycotoxins, heavy metals and somatotropin hormone. Antibiotics such as betalactams, tetracycline, amino glycosides (e.g. streptomycin, neomycin and gentamycin), macrolides (e.g. erythromycin) and sulfonamides are the source of residues. Because of the public health significance, milk and milk products contaminated with antibiotics beyond a given residue levels, considered unfit for human consumption. Problems associated with antibiotic residues in milk include the risk of allergic reactions and occurrence of antibioticresistant bacteria, teratogenicity risk to the foetus, hypoplasia in developing teeth, aplasia of bone marrow, chronic insidious intake lead to elevated cancer risk and disruption of body's reproductive, immune, endocrine and nervous system. Organochlorine pesticides are substances containing contaminants classes: The dichlorodiphenylethanes, the chlorinated cyclodienes (aldrin, dieldrin, heptachlor, etc.), and the hexachlorocyclohexanes (lindane). The most common effects of the wide spread of organochlorine compounds in the environment are birth defects, neurological effects, behavioral effects, reproductive effects and cancer. The occurrence of chemical residues in the milk of lactating cows is a matter of public health concern, since dairy products are widely consumed by infants, children and many adults throughout the world. Governments have responsibility for making regulations to protect consumers against harm arising from chemical in food. The regulation of illegal residues in foods is a cooperative effort of Food Safety and Inspection Services, Feed and Drug Administration and Environmental Protection Agency. However, inEthiopia not yet attention given regarding to set standards, control measures and monitoring program of chemical residues in animal originated foodstuff.

show abstract

Validation of a confirmatory method for the determination of sulphonamides in muscle according to the European Union regulation 2002/657/EC

Cited by 73 publications

References 15 publications

Effects of various freezing and cooking processes on the residues of sulfamethazine in broiler tissues

Effects of various freezing and cooking processes on the residues of sulfamethazine in broiler tissues

Otimização e validação de método multirresíduo para determinação de sulfonamidas em camarão cultivado por cromatografia líquida de alta eficiência com detecção por UV

Review on Chemical Residues in Milk and Their Public Health Concern in Ethiopia

Contact Info

Product

Resources

About