Validation and Usefulness of the Sperm Quality Analyzer (SQA II‐C) for Bull Semen Analysis

Hoflack, Geert; Rijsselaere, Tom; Maes, Dominiek; Dewulf, Jeroen; Opsomer, G.; Kruif, Aart de; Soom, Ann Van

doi:10.1111/j.1439-0531.2005.00584.x

Cited by 14 publications

(11 citation statements)

References 39 publications

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“…High repeatability of measurements was observed for all parameters assessed, since the CV's of the results remained below 10%. These findings are in agreement with findings in previous studies in dogs [Iguer-Ouada and Verstegen 2001b;Rijsselaere et al 2003;Schäfer-Somi and Aurich 2007] and bulls [Hoflack et al 2005] using different types of CASA systems. It is, however, important to emphasize that variations of 30 -60% were obtained when the motility of the same ejaculate was assessed using subjective microscopy by different observers within the same laboratory [Verstegen et al 2002].…”

Section: Discussionsupporting

confidence: 83%

Influence of sampling factors on canine sperm motility parameters measured by the Sperm Class Analyzer

Dorado

Rijsselaere

Muñoz-Serrano

et al. 2011

Systems Biology in Reproductive Medicine

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The aim of the present study was to investigate the effect of different technical settings and semen processing on sperm motility parameters measured by the Sperm Class Analyzer (SCA). Semen was collected from 3 dogs, pooled, and diluted in phosphate buffered saline and subsequently assessed by the SCA for the different sperm motility characteristics. The data were statistically analyzed by ANOVA and the repeatability was assessed by coefficient of variation (CV). After a principal component analysis, the reliability was determined with intraclass correlation coefficient (ICC). In experiment 1, the CV's were below 10% for all evaluated parameters. Significant differences (P < 0.05) were found between the different sperm concentrations (25, 50, and 75 x 10 6 spermatozoa/ml) in all of the motion parameters assessed, yielding the highest ICC (0.81) at 25 x 10 6 spermatozoa/ml. No significant differences (P > 0.05) in SCA read-outs were found between the number of microscopic fields captured (1, 2, 3, 4, or 5 fields), yielding the highest ICC (0.83) when 3 fields were captured. No significant differences (P > 0.05) in motility parameters were found between the number of cells analyzed in each field (20, 50, and 100 spermatozoa) with the exception of beat cross frequency. Reliability of the SCA was good (ICC = 0.71 to 0.90) for all motility measurements when 20 (ICC = 0.89) or 50 (ICC = 0.77) cells were captured in each field, but only just acceptable (ICC = 0.51 to 0.70) when 100 cells were counted (ICC = 0.67). The frame settings significantly (P < 0.05) influenced most of the measured motility characteristics. Scanning 60 frames at a frame rate of 30 Hz improved the reliability of the results (ICC = 0.92). In conclusion, we suggest that the measurements with the SCA are ideally performed at a sperm concentration of 25 x 10 6 spermatozoa/ml, counting at least 100 cells in three microscopic fields. We also propose that the SCA should analyze 60 frames at 30 Hz to yield consistent results of a set of measurements or a measuring instrument thus obtaining reliable motility results.

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Section: Discussionsupporting

confidence: 83%

Influence of sampling factors on canine sperm motility parameters measured by the Sperm Class Analyzer

Dorado

Rijsselaere

Muñoz-Serrano

et al. 2011

Systems Biology in Reproductive Medicine

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“…The following results are presented in Table 2 and Fig. 2 The rapid motile spermatozoa had higher values for velocity and progressiveness (VCL, VAP, VSL, ALH, STR and LIN) as similar patterns were observed by Hoflack et al (2005) in Holstein Friesian bulls. The spermatozoa in medium motile subpopulations although having straight line progression had poor velocity (low VAP, VCL, ALH and BCF).…”

Section: Resultssupporting

confidence: 67%

Understanding motility dynamics of crossbred bull spermatozoa when analyzed by Computer Assisted Semen Analyzer (CASA)

Rai¹,

Tyagi²,

Kumar³

et al. 2017

IJAR

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The study was conducted to understand sperm kinetics of Frieswal bull spermatozoa using Computer Assisted Semen Analyzer (CASA). Fifty bull ejaculates were collected from ten healthy bulls that were in routine semen collection. The semen samples were diluted in Tris buffer at a concentration of 25x10 6 spermatozoa/ ml for analysis. Rapidly moving spermatozoa represented the one with better velocity and progressiveness while spermatozoa with medium motility had low velocity with short distance travelled in spite of its ability to move in a straight line (straightness, STR >70 %). The slow moving cells had good head and flagella movement but they followed a circular path with straight line velocity (VSL m/ sec), linearity (LIN %) and straightenss (STR %) of 10.91, 9.00 and 22.54, respectively. Results indicated that individual sperm cells tracked by CASA as rapid, medium and slow motile were highly variable (p<0.001). However, the sperm motility between bulls varied (p<0.05) only in lateral head displacement (ALH, m) and beat cross frequency (BCF, Hz).

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“…Dilution of these ejaculates is necessary for accurate assessment of sperm quality. A decline in sperm motility as concentration increases has been reported in studies on bovine and human semen with automated semen analyzers (Bartoov et al, 1991; Hoflack et al, 2005). Collision between cells and restriction in movement from being in a condensed state were suggested to be likely causes.…”

Section: Discussionmentioning

confidence: 91%

Semen Collection and Ejaculate Characteristics in the Indo‐Pacific Bottlenose Dolphin (Tursiops aduncus)

Yuen

Brook

Kinoshita³

et al. 2009

Journal of Andrology

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The testes of sexually mature dolphins produce large quantities of sperm. Sperm are expelled in copious amounts of seminal fluid through repeated ejaculations that can occur within a short period of time. There is currently a lack of detail about the methodology of sequential ejaculate collection in dolphins and how the traits of individual ejaculates affect the assessment of reproductive capacity. The objectives of this study were to obtain series of ejaculates from 3 dolphins by following a well-defined collection protocol and then to characterize individual ejaculates. Semen was collected weekly for 1 year from 3 sexually mature Tursiops aduncus. The end of a collection session was marked by a lack of semen, in spite of effort, or micturition. Individual ejaculates were analyzed for volume, pH, and sperm concentration, count, motility, and viability following methods previously described for dolphin semen.The first ejaculate was typically higher in volume and sperm count but lower in sperm concentration, motility, and viability. The concentration of the second ejaculate was generally the highest. Sperm motility and viability of the second and subsequent ejaculates in a series were good (ie, .80%). Collection of the first ejaculate only can lead to underestimation of reproductive capacity. Sperm output fluctuated erratically from week to week, so it was difficult to assess any seasonal pattern. Results provide information for further investigation of male dolphin reproductive patterns and any effect of collection frequency on ejaculate characteristics.

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Validation and Usefulness of the Sperm Quality Analyzer (SQA II‐C) for Bull Semen Analysis

Cited by 14 publications

References 39 publications

Influence of sampling factors on canine sperm motility parameters measured by the Sperm Class Analyzer

Influence of sampling factors on canine sperm motility parameters measured by the Sperm Class Analyzer

Understanding motility dynamics of crossbred bull spermatozoa when analyzed by Computer Assisted Semen Analyzer (CASA)

Semen Collection and Ejaculate Characteristics in the Indo‐Pacific Bottlenose Dolphin (Tursiops aduncus)

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