Validation and theoretical justification of an LC-MS method for the animal species specific detection of gelatin

Kleinnijenhuis, Anne J.; Holthoon, Frédérique L. van; Herregods, Griet

doi:10.1016/j.foodchem.2017.09.104

Cited by 32 publications

(22 citation statements)

References 17 publications

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“…Collagen tucks in a triple‐helical structure because of the specific abundance of Hyp, Pro, and Gly. [ 16 ] Commercial gelatin or CH is mainly made from hydrolyzed type 1 collagen (to date, 28 types of collagen have been found) because type 1 collagen is normally from skin, vasculature, tendon, bone and connective tissues, [ 17,18 ] composed of two α 1 chains and one α 2 chain. [ 14 ] So far, this material has been widely applied in the cosmetics, photography, food, and pharmaceutical industries; [ 19 ] it is also used as a gelatinized electrolyte for batteries, due to its hydrophilic, gummy, elastic and stable features.…”

Section: Characterization Of Materials and Electrodesmentioning

confidence: 99%

Mn²⁺ Ions Confined by Electrode Microskin for Aqueous Battery beyond Intercalation Capacity

Liu

Zhi

Sedighi

et al. 2020

Advanced Energy Materials

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In aqueous rechargeable zinc–manganese dioxide batteries (ZMBs), some irreversible side reactions, such as Mn2+ dissolution, often lead to capacity fading over cycling. These side reactions play a crucial role in the capacity and cycle performance of the battery. The implementation of a bionic electrode microskin (EMS) composed of collagen hydrolysate to convert the irreversible side reactions into reversible reactions is reported. The proposed EMS effectively adsorbs and confines the Mn2+ ions around the cathode through van der Waals forces, hydrogen bonds, and/or ionic interactions, which makes the MnO2/Mn2+ reactions reversible during the charge/discharge process. Such Mn2+ dissolution reactions, with an ultrahigh theoretical capacity (617 mAh g‐1), contribute a large amount of capacity, ≈44% of the total specific capacity at a low scan rate. Based on these fundamental findings, the assembled ZMBs with an EMS display an unprecedented discharge capacity of 415 mAh g‐1 at 20 mA g‐1, which overcomes the theoretical capacity (308 mAh g‐1) limitation of the Zn2+ intercalation mechanism. More significantly, the EMS on all α‐, β‐, and γ‐MnO2 cathodes exhibits similar high capacity beyond the theoretical capacity of Zn intercalation and capacity retention enhancement after 3000 cycles.

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Section: Characterization Of Materials and Electrodesmentioning

confidence: 99%

Mn²⁺ Ions Confined by Electrode Microskin for Aqueous Battery beyond Intercalation Capacity

Liu

Zhi

Sedighi

et al. 2020

Advanced Energy Materials

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“…With the new “Species by Proteome INvestigation” (SPIN) workflow, we made use of the latest advances in LC-MS/MS to achieve fast and confident global species identification. Shotgun proteomics has the advantage of coping well with highly-degraded 14 or processed 15 specimens and for SPIN, we pushed the throughput beyond the fastest published methods 16 and developed a universal species inference algorithm for reproducible data interpretation. We developed and benchmarked a single-step method to extract proteins from mineralized tissues, which is followed by automated contaminant removal and digestion by protein aggregation capture (PAC).…”

Section: Introductionmentioning

confidence: 99%

“…We decided to optimize and test the SPIN work-flow for small 5 mg mammalian bone samples, as these are relevant for many potential use cases, such as forensic screening of animal or human remains, 21 archaeological investigations of bones and tools, 22, 23 authentication of processed food like gelatins, 15 and analyzing the change of species diversity in ecosystems. 24, 25 We optimized our methods with reference bones from 9 domesticated animals, a human, and 3 other great apes, validated the reproducibility and benchmarked comparability to morphologybased identification with more than 60 partially degraded bones from the Danish Early Iron-Age, and stress-tested the approach with a set of over 200 Middle and Upper Palaeolithic bone fragments from three archaeological sites in Portugal dating to 30 - 60,000 BP.…”

Section: Introductionmentioning

confidence: 99%

SPIN - Species by Proteome INvestigation

Ruether

Husic

Bangsgaard

et al. 2021

Preprint

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Genetic species determination has become an indispensable tool in forensics, archaeology, ecology, and food authentication. The available methods are either suited for detecting a single taxon across many samples or for screening a wide range of species across a few samples. Here, we introduce "Species by Proteome INvestigation" (SPIN), a proteomics workflow capable of querying over 150 mammalian species in 7.2 minutes of mass spectrometry (MS) analysis. Streamlined and automated sample preparation by protein aggregation capture, high-speed chromatography and data-independent acquisition, and a confident species inference algorithm facilitate processing hundreds of samples per day. We demonstrate the correct classification of known references, reproducible species identification in degraded Iron-Age material from Scandinavia, and test the limits of our methods with Middle and Upper Palaeolithic bones from Southern European sites with late Neanderthal occupation. While this initial study is focused on modern and archaeological mammalian bone, SPIN will be open and expandable with other biological tissues and taxa.

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“…when new relevant analytes are defined or discovered after synthesis of the labeled library. In addition, it is very difficult to confirm the concentrations of all the constituents of the library, which brings us back to approaches wherein labeled analytes are synthesized individually 14 . Individual synthesis can be expensive, especially when multiple components are analyzed.…”

Section: Introductionmentioning

confidence: 99%

Reliable and generic liquid chromatography/mass spectrometry quantification of short peptides using a stable‐isotope‐labeled labeling agent

Kleinnijenhuis¹,

Vergauwen²,

Holthoon³

et al. 2020

Rapid Comm Mass Spectrometry

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Rationale It is important to investigate the behavior of protein hydrolysate components in both in vitro and in vivo studies, to support the elucidation of their biological functions. As protein hydrolysates and biological matrices are highly complex mixtures, it is essential to apply fully reliable and flexible analytical approaches. Methods A novel and generic Liquid Chromatography/Mass Spectrometry methodology was developed to analyze short peptides. A stable‐isotope‐labeled labeling agent 6‐aminoquinolyl‐N‐hydroxysuccinimidyl carbamate (13C3) was synthesized and used to prepare internal standards from non‐labeled analyte peptides. The amino acid and peptides p, pG, Pp, GPp and PpG (where p stands for hydroxyproline) were used for proof of principle. Results The method showed acceptable performance in solvent, in simulated gastrointestinal fluid and in serum. The (linear) dynamic range expanded to over four orders of magnitude, which is very useful when multiple analytes are analyzed in a biological matrix, due to the large differences in concentrations observed for endogenous and protein hydrolysate components. The method provides absolute‐quantitative results and is fully accountable on the single‐sample and single‐component level. Conclusions The methodology can be applied to reliably quantify protein hydrolysate nutraceutical components at various stages during their in vivo processing. Internal standards can also be synthesized for other short peptides whenever they are expected to have biological relevance and require quantification. Overall this provides an excellent analytical tool to support the elucidation of the biological functions of protein hydrolysate components.

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Validation and theoretical justification of an LC-MS method for the animal species specific detection of gelatin

Cited by 32 publications

References 17 publications

Mn²⁺ Ions Confined by Electrode Microskin for Aqueous Battery beyond Intercalation Capacity

Mn²⁺ Ions Confined by Electrode Microskin for Aqueous Battery beyond Intercalation Capacity

SPIN - Species by Proteome INvestigation

Reliable and generic liquid chromatography/mass spectrometry quantification of short peptides using a stable‐isotope‐labeled labeling agent

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Validation and theoretical justification of an LC-MS method for the animal species specific detection of gelatin

Cited by 32 publications

References 17 publications

Mn2+ Ions Confined by Electrode Microskin for Aqueous Battery beyond Intercalation Capacity

Mn2+ Ions Confined by Electrode Microskin for Aqueous Battery beyond Intercalation Capacity

SPIN - Species by Proteome INvestigation

Reliable and generic liquid chromatography/mass spectrometry quantification of short peptides using a stable‐isotope‐labeled labeling agent

Contact Info

Product

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Mn²⁺ Ions Confined by Electrode Microskin for Aqueous Battery beyond Intercalation Capacity

Mn²⁺ Ions Confined by Electrode Microskin for Aqueous Battery beyond Intercalation Capacity