The cryogenic transfer line for KATRIN connects 3 large superconducting magnet systems in the Tritium Laboratory Karlsruhe (TLK) with a He refrigerator installed in an external machine building. The vacuum-insulated transfer line comprises 1 N 2 and 5 He process lines that are surrounded by a LN 2 -cooled radiation shield. Covering a distance of 40 m, the transfer line includes 4 valve boxes, 1 T-junction, 1 vertical and 3 horizontal line sections. After the introductory system description, an overview is given on project coordination and quality assurance, together with results of leak testing during pre-acceptance and commissioning. The method and results of thermal loss measurements in the He system are explained, reaching an accuracy of ±30 % with standard process instrumentation. Potential improvements in the MLI insulation scheme are revealed, based on both the measurements and theoretical considerations. The experimental results are finally compared with data of large cryogenic transfer systems.