Validation and long-term assessment of an approach for the high throughput determination of lipophilicity (logPOW) values using multiplexed, absorbance-based capillary electrophoresis

Recent advances in microemulsion electrokinetic chromatography

“…The MEEKC method of octanol-water partition coefficient determination has recently been demonstrated for a series of synthetic steroids [2], small organic molecules [4], immunosuppressive drugs [5], biphenyl nitrile compounds used in the synthesis of liquid crystals [6], pesticides [7], and for weakly acidic and neutral compounds using a dynamic capillary coating [61]. The use of MEEKC for log P determinations has also been demonstrated with a 96-capillary array instrument for the high-throughput screening of compound hydrophobicity [1], and by pressure-assisted MEEKC [57]. Based on the same partitioning principle used to determine log P OW of compounds, the interaction between drug molecules and vehicle systems has been characterised using MEEKC [8].…”

“…Solutes interact with the migrating droplets and the chromatographic interaction is related to their hydrophobicity which enables the separation of neutral species. Indeed, MEEKC has been used [1][2][3][4][5][6][7][8] to assess compound hydrophobicity with good cross-correlation to other hydrophobicity measurement techniques. Charged solutes may also have a charge repulsion or ion-association with the droplet which will also affect their migration times.…”

Recent advances in the development and application of microemulsion EKC

“…Examples of recent high-throughput applications of multiplexed MEEKC include estimation of log P ow values [80] using microchip MEEKC with induced fluorescence detection. Wehmeyer et al [81], Marsh and Altria [82] and Wong et al [83], have reported the use of multiplexed MEEKC for the determination of log P values. Marsh and Altria [82] in a recent paper reported the use of this method for a number of applications including, tablet assay and impurities determination, pK a determinations and log P determinations.…”

“…Recent applications of MEEKC to determine physicochemical properties of compounds have included microchip MEEKC coupled with LIFD [80], which was shown to provide a 'proof of concept' for employing microchip MEEKC-LIFD for log P determinations. The use of multiplexed MEEKC systems [81,82,83] has also been shown to provide rapid determinations of physicochemical properties for a large number of drugs and chemical compounds. See Table 5 for recent MEEKC log P applications.…”

Capillary electrophoresis for the analysis of small‐molecule pharmaceuticals

“…The use of MEEKC to determine compound solubility (log P) has been successfully shown for many types of drugs [21][22][23][24][25][26][27][28][29] and is an important pharmaceutical application of the technique.…”