Validation and application of a simple and rapid stability-indicating liquid chromatographic assay for the quantification of caffeine from human saliva

Oliphant, Elizabeth Anne; Purohit, Trusha J.; Alsweiler, Jane; McKinlay, Christopher J.D.; Hanning, Sara M.

doi:10.1080/10826076.2022.2095402

Cited by 6 publications

(4 citation statements)

References 20 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In addition, the criteria outlined in the tutorial review on validation of LC-MS methods by Kruve et al were followed [27,28]. Articles on validation of analytical methods for the determination of amines or amides by LC-MS were also consulted [20,[29][30][31][32][33].…”

Section: Methods Validationmentioning

confidence: 99%

Development and validation of a liquid chromatography‐mass spectrometry method for quantification of octadecylamine in the secondary circuit of a nuclear power plant in the presence of other amines

Bosco Ackerman,

Martín,

Espinosa

et al. 2024

Separation Science Plus

View full text Add to dashboard Cite

Octadecylamine (ODA) is the most widely used film‐forming amine in the nuclear industry. Its function is to minimize corrosion. In this work, we developed and validated a liquid chromatography‐mass spectrometry method that allows the quantification of ODA in the presence of other amines in the secondary system of nuclear power plants operating under all‐volatile treatment chemistry. Sample stabilities greater than 95% were achieved after 1 week of being taken in acetonitrile 1:1. The method showed a high selectivity allowing the measurement of ODA in the presence of other amines. The calibration curve was linear in the range of 0.0025–0.1025 mg/L. The limit of detection and limit of quantification (LOQ) were 1.8 and 2.2 µg/L, respectively for an injection volume of 3 µL. Lower LOQs can be achieved by increasing the injection volume, making the method applicable even in the first hours and days of treatment. The bias of the method was less than 10% and the relative standard deviation of reproducibility was less than 15%. The sample stabilization was robust and the uncertainty associated with the method was acceptable. The method was applied to a real sample taken after a few years of treatment and a concentration of 0.15 mg/L was measured.

show abstract

Section: Methods Validationmentioning

confidence: 99%

Development and validation of a liquid chromatography‐mass spectrometry method for quantification of octadecylamine in the secondary circuit of a nuclear power plant in the presence of other amines

Bosco Ackerman,

Martín,

Espinosa

et al. 2024

Separation Science Plus

View full text Add to dashboard Cite

show abstract

“…Saliva samples were collected from mothers (three samples across an 8-hour daytime period) and infants (prior to the study drug) at the 2-week timepoint and analysed to determine caffeine concentrations. 32 …”

Section: Methodsmentioning

confidence: 99%

Caffeine to prevent intermittent hypoxaemia in late preterm infants: randomised controlled dosage trial

Oliphant

McKinlay

McNamara

et al. 2022

Arch Dis Child Fetal Neonatal Ed

Self Cite

View full text Add to dashboard Cite

ObjectiveTo establish the most effective and best tolerated dose of caffeine citrate for the prevention of intermittent hypoxaemia (IH) in late preterm infants.DesignPhase IIB, double-blind, five-arm, parallel, randomised controlled trial.SettingNeonatal units and postnatal wards of two tertiary maternity hospitals in New Zealand.ParticipantsLate preterm infants born at 34+0–36+6 weeks’ gestation, recruited within 72 hours of birth.InterventionInfants were randomly assigned to receive a loading dose (10, 20, 30 or 40 mg/kg) followed by 5, 10, 15 or 20 mg/kg/day equivolume enteral caffeine citrate or placebo daily until term corrected age.Primary outcomeIH (events/hour with oxygen saturation concentration ≥10% below baseline for ≤2 min), 2 weeks postrandomisation.Results132 infants with mean (SD) birth weight 2561 (481) g and gestational age 35.7 (0.8) weeks were randomised (24–28 per group). Caffeine reduced the rate of IH at 2 weeks postrandomisation (geometric mean (GM): 4.6, 4.6, 2.0, 3.8 and 1.7 events/hour for placebo, 5, 10, 15 and 20 mg/kg/day, respectively), with differences statistically significant for 10 mg/kg/day (GM ratio (95% CI] 0.39 (0.20 to 0.76]; p=0.006) and 20 mg/kg/day (GM ratio (95% CI] 0.33 (0.17 to 0.68]; p=0.003) compared with placebo. The 20 mg/kg/day dose increased mean (SD) pulse oximetry oxygen saturation (SpO2) (97.2 (1.0) vs placebo 96.0 (0.8); p<0.001), and reduced median (IQR) percentage of time SpO2 <90% (0.5 (0.2–0.8) vs 1.1 (0.6–2.4); p<0.001) at 2 weeks, without significant adverse effects on growth velocity or sleeping.ConclusionCaffeine reduces IH in late preterm infants at 2 weeks of age, with 20 mg/kg/day being the most effective dose.Trial registration numberACTRN12618001745235.

show abstract

“…A popular method in chemical, medical or pharmaceutical analysis is high-performance liquid chromatography, and therefore, many analyses of caffeine, paracetamol and propyphenazone are carried out using this method. The analyses concern single compounds or multi-component mixtures, also with substances other than those mentioned [7][8][9][10][11][12][13][14][15][16]. Most often, these are preparations available on the pharmaceutical market.…”

Section: Introductionmentioning

confidence: 99%

Comparison of the Limit of Detection of Paracetamol, Propyphenazone, and Caffeine Analyzed by TLC and HPTLC

Bober-Majnusz,

Pyka-Pająk

2024

Preprint

View full text Add to dashboard Cite

TLC and HPTLC in normal (NP) and reversed (RP) phase systems combined with densitometry were used to analyze caffeine, propyphenazone and paracetamol. Normal phase analyses were performed on five different stationary phases (chromatographic plates), testing three mobile phases. Reversed-phase analyses were performed on four different chromatographic plates using three mobile phases. Thus, it was checked whether analyses using TLC chromatographic plates could be as sensitive as those performed on HPTLC plates. For all analyzed biologically active substances, the limit of detection (LOD) was calculated, i.e. a parameter that describes the method sensitivity. It has been shown that using both TLC and HPTLC plates, it is possible to develop chromatographic conditions that enable the detection of caffeine, propyphenazone and paracetamol in amounts ranging from a dozen to several dozen µg/spot. In the reversed-phase (RP) system, lower LOD values for all tested compounds were obtained using TLC than HPTLC. However, using analyses in the normal phase system (NP), similar (of the same order) LOD values are obtained for caffeine, propyphenazone and paracetamol, both when using TLC and HPTLC plates. Therefore, for economic reasons, TLC plates should be recommended for analyses of caffeine, propyphenazone and paracetamol, which are several times cheaper than HPTLC plates.

show abstract

Validation and application of a simple and rapid stability-indicating liquid chromatographic assay for the quantification of caffeine from human saliva

Cited by 6 publications

References 20 publications

Development and validation of a liquid chromatography‐mass spectrometry method for quantification of octadecylamine in the secondary circuit of a nuclear power plant in the presence of other amines

Development and validation of a liquid chromatography‐mass spectrometry method for quantification of octadecylamine in the secondary circuit of a nuclear power plant in the presence of other amines

Caffeine to prevent intermittent hypoxaemia in late preterm infants: randomised controlled dosage trial

Comparison of the Limit of Detection of Paracetamol, Propyphenazone, and Caffeine Analyzed by TLC and HPTLC

Contact Info

Product

Resources

About