Validating the potential of double-stranded RNA targeting Colorado potato beetle <i>mesh</i> gene in laboratory and field trials

Petek, Marko; Coll, Anna; Ferenc, Rok; Razinger, Jaka; Gruden, Kristina

doi:10.1101/2020.02.13.945097

Cited by 7 publications

(6 citation statements)

References 46 publications

(32 reference statements)

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“…Petek at al. [ 86 ] in their study designed dsRNA to silence the CPB mesh gene ( MESH ). They did laboratory-feeding trials to assess impacts on beetle survival and development and also a field trial to compare dsRNA sprayed potato with a spinosad-based insecticide.…”

Section: New Approaches To Colorado Potato Beetle Managementmentioning

confidence: 99%

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Modern Techniques in Colorado Potato Beetle (Leptinotarsa decemlineata Say) Control and Resistance Management: History Review and Future Perspectives

Balaško

Mikac

Bažok

et al. 2020

Insects

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Colorado potato beetle, CPB (Leptinotarsa decemlineata Say), is one of the most important pests of the potato globally. Larvae and adults can cause complete defoliation of potato plant leaves and can lead to a large yield loss. The insect has been successfully suppressed by insecticides; however, over time, has developed resistance to insecticides from various chemical groups, and its once successful control has diminished. The number of available active chemical control substances is decreasing with the process of testing, and registering new products on the market are time-consuming and expensive, with the possibility of resistance ever present. All of these concerns have led to the search for new methods to control CPB and efficient tools to assist with the detection of resistant variants and monitoring of resistant populations. Current strategies that may aid in slowing resistance include gene silencing by RNA interference (RNAi). RNAi, besides providing an efficient tool for gene functional studies, represents a safe, efficient, and eco-friendly strategy for CPB control. Genetically modified (GM) crops that produce the toxins of Bacillus thuringiensis (Bt) have many advantages over agro-technical, mechanical, biological, and chemical measures. However, pest resistance that may occur and public acceptance of GM modified food crops are the main problems associated with Bt crops. Recent developments in the speed, cost, and accuracy of next generation sequencing are revolutionizing the discovery of single nucleotide polymorphisms (SNPs) and field of population genomics. There is a need for effective resistance monitoring programs that are capable of the early detection of resistance and successful implementation of integrated resistance management (IRM). The main focus of this review is on new technologies for CPB control (RNAi) and tools (SNPs) for detection of resistant CPB populations.

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Section: New Approaches To Colorado Potato Beetle Managementmentioning

confidence: 99%

“…Petek at al. [ 86 ] showed in laboratory trials as well as in the field that spraying with insecticidal dsRNA is a highly efficient strategy for managing CPB. Future research will have to focus on formulations to improve dsRNA stability and cellular uptake.…”

Section: New Approaches To Colorado Potato Beetle Managementmentioning

confidence: 99%

Modern Techniques in Colorado Potato Beetle (Leptinotarsa decemlineata Say) Control and Resistance Management: History Review and Future Perspectives

Balaško

Mikac

Bažok

et al. 2020

Insects

View full text Add to dashboard Cite

show abstract

“…Meanwhile, there are also promising non-transformative RNAi applications/approaches available. RNAi via sprayable or soaking approaches has been recognized as a potential strategy for pest control, and thereafter, it has been well studied in a number of insect species (Avila et al, 2018;Petek et al, 2020). However, the RNAi e ciency can greatly vary among different insect species.…”

Section: Introductionmentioning

confidence: 99%

A first greenhouse application of bacteria-expressed and nanocarrier-delivered RNA pesticide for Myzus persicae control

Zhang

et al. 2022

J Pest Sci

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There are two main limitations for sprayable RNA pesticide development: delivery e ciency and synthetic cost of double-stranded RNA (dsRNA). We previously constructed a nanocarrier-based transdermal dsRNA delivery system and a novel bacteria-based hairpin RNA (hpRNA) expression system to solve these challenges. Herein, as a subsequent exploration of RNA pesticide (sprayable ds/hpRNA for pest control), we performed a greenhouse application of bacteria-expressed and nanocarrier-delivered RNA pesticide on green peach aphid. The nanoparticle SPc could combine and deliver dsRNA across the aphid cuticle, and V-type proton ATPase subunits d (ATP-d) and G (ATP-G) were selected as the potential RNA interference (RNAi) targets. Our plasmid-Escherichia coli system simultaneously expressing ATP-d and ATP-G hairpin RNAs (hpRNAs) was constructed for mass production of hpRNA. The expressed hpRNA was mixed with SPc and detergent to form RNA formulation, which showed a certain insecticidal activity through the spray application in the greenhouse. Total control e cacy of our RNA pesticide could reach 61% on 3 d, and maintained at 50% until the sixth day. To our knowledge, our study is the rst attempt to apply the bacteria-expressed and nanocarrier-delivered RNA pesticides for pest control in the greenhouse trial, which is bene cial for promoting the development of RNA pesticides. Key Message• SPc can protect and deliver dsRNA from degradation for e cient gene silencing• hpRNA is expressed in E. coli system to develop a novel RNA insecticide • Our RNA pesticide can be applied to control aphids in greenhouse • Our study brings the concept of RNAi-based pest management to practical application

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“…The scope of this project was to develop double-stranded RNA (dsRNA) based insecticide against Colorado potato beetle pest. It included identification of Colorado potato beetle gene targets, establishing dsRNA production, and validating the dsRNAs' insecticidal potential in laboratory and field trials 23 . The directory tree of this project consists of three investigations covering altogether 16 studies with wet-lab and dry-lab assays (Fig.…”

Section: Integration Of Pisa-tree Into Web-based Data Management Syst...mentioning

confidence: 99%

pISA-tree - a data management framework for life science research projects using a standardised directory tree

et al. 2022

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We developed pISA-tree, a straightforward and flexible data management solution for organisation of life science project-associated research data and metadata. pISA-tree was initiated by end-user requirements thus its strong points are practicality and low maintenance cost. It enables on-the-fly creation of enriched directory tree structure (project/Investigation/Study/Assay) based on the ISA model, in a standardised manner via consecutive batch files. Templates-based metadata is generated in parallel at each level enabling guided submission of experiment metadata. pISA-tree is complemented by two R packages, pisar and seekr. pisar facilitates integration of pISA-tree datasets into bioinformatic pipelines and generation of ISA-Tab exports. seekr enables synchronisation with the FAIRDOMHub repository. Applicability of pISA-tree was demonstrated in several national and international multi-partner projects. The system thus supports findable, accessible, interoperable and reusable (FAIR) research and is in accordance with the Open Science initiative. Source code and documentation of pISA-tree are available at https://github.com/NIB-SI/pISA-tree.

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Validating the potential of double-stranded RNA targeting Colorado potato beetle mesh gene in laboratory and field trials

Cited by 7 publications

References 46 publications

Modern Techniques in Colorado Potato Beetle (Leptinotarsa decemlineata Say) Control and Resistance Management: History Review and Future Perspectives

Modern Techniques in Colorado Potato Beetle (Leptinotarsa decemlineata Say) Control and Resistance Management: History Review and Future Perspectives

A first greenhouse application of bacteria-expressed and nanocarrier-delivered RNA pesticide for Myzus persicae control

pISA-tree - a data management framework for life science research projects using a standardised directory tree

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