Validating genome‐wide associated signals for clinical mastitis in German Holstein cattle

Abdel-Shafy, Hamdy; Bortfeldt, Ralf; Reißmann, Monika; Brockmann, Gudrun A.

doi:10.1111/age.12624

Cited by 14 publications

(15 citation statements)

References 24 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Few regions on BTA6 and BTA14 are known to affect SCS and mastitis (Ogorevc et al, 2009;Wang et al, 2015). Within BTA6, several signals covered a region from 84.57 to 88.82 Mb, coincident to the window already reported to affect mastitis or SCS in US Holsteins (Cole et al, 2011), Nordic Holsteins (Sahana et al, 2013), Danish Holsteins (Wu et al, 2015), and German Holsteins (Abdel-Shafy et al, 2018). Furthermore, a variant in position 88.82 Mb of BTA6 was very close to CXCL8 (88.81 Mb), a gene related to mastitis, IMI, immune and inflammatory responses, and neutrophil activation (Youngerman et al, 2004;NCBI, 2018).…”

Section: Lactose Yieldsupporting

confidence: 67%

On the genomic regions associated with milk lactose in Fleckvieh cattle

Costa

Schwarzenbacher²,

Mészáros

et al. 2019

Journal of Dairy Science

View full text Add to dashboard Cite

Lactose is a sugar uniquely found in mammals' milk and it is the major milk solid in bovines. Lactose yield (LY, kg/d) is responsible for milk volume, whereas lactose percentage (LP) is thought to be more related to epithelial integrity and thus to udder health. There is a paucity of studies that have investigated lactose at the genomic level in dairy cows. This paper aimed to improve our knowledge on LP and LY, providing new insights into the significant genomic regions affecting these traits. A genome-wide association study for LP and LY was carried out in Fleckvieh cattle by using bulls' deregressed estimated breeding values of first lactation as pseudo-phenotypes. Heritabilities of firstlactation test-day LP and LY estimated using linear animal models were 0.38 and 0.25, respectively. A total of 2,854 bulls genotyped with a 54K SNP chip were available for the genome-wide association study; a linear mixed model approach was adopted for the analysis. The significant SNP of LP were scattered across the whole genome, with signals on chromosomes 1, 2, 3, 7, 12, 16, 18, 19, 20, 28, and 29; the top 4 significant SNP explained 4.90% of the LP genetic variance. The signals were mostly in regions or genes with involvement in molecular intra-or extracellular transport; for example, CDH5, RASGEF1C, ABCA6, and SLC35F3. A significant region within chromosome 20 was previously shown to affect mastitis or somatic cell score in cattle. As regards LY, the significant SNP were concentrated in fewer regions (chromosomes 6 and 14), related to mastitis/somatic cell score, immune response, and transport mechanisms. The 5 most significant SNP for LY explained 8.45% of genetic variance and more than one-quarter of this value has to be attributed to the variant within ADGRB1. Significant peaks in target regions remained even after adjustment for the 2 most significant variants previously detected on BTA6 and BTA14. The present study is a prelude for deeper investigations into the biological role of lactose for milk secretion and volume determination, stressing the connection with genes regulating intra-or extracellular trafficking and immune and inflammatory responses in dairy cows. Also, these results improve the knowledge on the relationship between lactose and udder health; they support the idea that LP and its derived traits are potential candidates as indicators of udder health in breeding programs aimed to enhance cows' resistance to mastitis.

show abstract

Section: Lactose Yieldsupporting

confidence: 67%

On the genomic regions associated with milk lactose in Fleckvieh cattle

Costa

Schwarzenbacher²,

Mészáros

et al. 2019

Journal of Dairy Science

View full text Add to dashboard Cite

show abstract

“…In order to compare the results that we obtained in DSN with German Holstein, we used GWAS results previously obtained in our lab on a population of 2,400 German Holstein bulls. This population has been used and described in detail repeatedly (Zielke et al, 2011(Zielke et al, , 2013Abdel-Shafy et al, 2018).…”

Section: Populationsmentioning

confidence: 99%

Genomic Loci Affecting Milk Production in German Black Pied Cattle (DSN)

et al. 2021

View full text Add to dashboard Cite

German Black Pied cattle (DSN) is an endangered population of about 2,550 dual-purpose cattle in Germany. Having a milk yield of about 2,500 kg less than the predominant dairy breed Holstein, the preservation of DSN is supported by the German government and the EU. The identification of the genomic loci affecting milk production in DSN can provide a basis for selection decisions for genetic improvement of DSN in order to increase market chances through the improvement of milk yield. A genome-wide association analysis of 30 milk traits was conducted in different lactation periods and numbers. Association using multiple linear regression models in R was performed on 1,490 DSN cattle genotyped with BovineSNP50 SNP-chip. 41 significant and 20 suggestive SNPs affecting milk production traits in DSN were identified, as well as 15 additional SNPs for protein content which are less reliable due to high inflation. The most significant effects on milk yield in DSN were detected on chromosomes 1, 6, and 20. The region on chromosome 6 was located nearby the casein gene cluster and the corresponding haplotype overlapped the CSN3 gene (casein kappa). Associations for fat and protein yield and content were also detected. High correlation between traits of the same lactation period or number led to some SNPs being significant for multiple investigated traits. Half of all identified SNPs have been reported in other studies, previously. 15 SNPs were associated with the same traits in other breeds. The other associated SNPs have been reported previously for traits such as exterior, health, meat and carcass, production, and reproduction traits. No association could be detected between DGAT1 and other known milk genes with milk production traits despite the close relationship between DSN and Holstein. The results of this study confirmed that many SNPs identified in other breeds as associated with milk traits also affect milk traits in dual-purpose DSN cattle and can be used for further genetic analysis to identify genes and causal variants that affect milk production in DSN cattle.

show abstract

“…To identify genomic loci affecting SCS or clinical mastitis directly, many association studies were performed in different dairy herds [7][8][9]. In this study, we tested 10 SNPs that have been previously associated with SCS in German Holstein bulls and cows [7,8] for associations with SCS in Sudanese purebred Butana and Butana…”

Section: Introductionmentioning

confidence: 99%

“…x Holstein crossbred cattle. Five of those SNPs were also found to be associated with clinical mastitis [9]. If those SNPs would be significantly associated, they could also be used as genetic markers to improve resistance against mastitis in Sudanese purebred Butana and Butana x Holstein crossbred populations.…”

Section: Introductionmentioning

confidence: 99%

Validation of Somatic Cell Score-Associated SNPs from Holstein Cattle in Sudanese Butana and Butana X Holstein Crossbred Cattle

Elzaki

Korkuć

Arends

et al. 2021

Preprint

View full text Add to dashboard Cite

The Bos indicus zebu cattle Butana is the most commonly used indigenous dairy cattle breed in Sudan. In the last years, high-yielding Holstein dairy cattle were introgressed into Butana cattle to improve their milk yield and simultaneously keep their good adaption to extreme environmental conditions. With the focus on the improvement of milk production, other problems arose such as an increased susceptibility to mastitis. Thus, genetic selection for mastitis resistance should be considered to maintain healthy and productive cows. In this study, we tested 10 SNPs which had been associated with somatic cell score (SCS) in Holstein cattle for association with SCS in 37 purebred Butana and 203 Butana × Holstein crossbred cattle from Sudan. Animals were genotyped by allele-specific KASP assays and association analysis was performed using a linear mixed model. All 10 SNPs were segregating in the crossbred Butana x Holstein populations, but only 8 SNPs in Sudanese purebred Butana cattle. The SNP on chromosome 13 was suggestively associated with SCS in the Butana x Holstein crossbred population (rs109441194, 13:79,365,467, PBF = 0.054) and the SNP on chromosome 19 was significantly associated with SCS in both populations (rs41257403, 19:50,027,458, Butana: PBF=0.003, Butana x Holstein: PBF=6.2x10-16). The minor allele of both SNPs showed an increasing in SCS. Therefore, selection against the disadvantageous minor allele could be used for genetic improvement of mastitis resistance in the studied populations. However, investigations in a bigger population and across the whole genome are needed to identify additional genomic loci.

show abstract

Validating genome‐wide associated signals for clinical mastitis in German Holstein cattle

Cited by 14 publications

References 24 publications

On the genomic regions associated with milk lactose in Fleckvieh cattle

On the genomic regions associated with milk lactose in Fleckvieh cattle

Genomic Loci Affecting Milk Production in German Black Pied Cattle (DSN)

Validation of Somatic Cell Score-Associated SNPs from Holstein Cattle in Sudanese Butana and Butana X Holstein Crossbred Cattle

Contact Info

Product

Resources

About