Validated UPLC–MS/MS method for determination of hordenine in rat plasma and its application to pharmacokinetic study

Ma, Jianshe; Wang, Shuanghu; Huang, Xueli; Geng, Peiwu; Wen, Congcong; Zhou, Yunfang; Yu, Linsheng; Wang, Xianqin

doi:10.1016/j.jpba.2015.03.032

Cited by 52 publications

(33 citation statements)

References 32 publications

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“…It is crucial to efficient remove the proteins and other potential interference in the bio-samples prior to LC-MS analysis in the method development [20][21][22]. Initially, a simple protein precipitation by acetonitrile-methanol (9:1, v/v) was investigated.…”

Section: Methods Developmentmentioning

confidence: 99%

“…Calibration plots were built in the range of 2-2000 ng/mL for berberrubine in rat plasma (2,5,10,20,50,100,200, 500, 1000 and 2000 ng/mL) and tissue (5,10,20,50,100,200, 500, 1000 and 2000 ng/mL). 10 L of the appropriate working solution would be added to 100 L of blank plasma or tissue, and followed by short vortex mixing.…”

Section: Calibration Standards and Quality Control Samplesmentioning

confidence: 99%

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Pharmacokinetics in rats and tissue distribution in mouse of berberrubine by UPLC-MS/MS

Wang

et al. 2015

Journal of Pharmaceutical and Biomedical Analysis

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Section: Methods Developmentmentioning

confidence: 99%

Section: Calibration Standards and Quality Control Samplesmentioning

confidence: 99%

Pharmacokinetics in rats and tissue distribution in mouse of berberrubine by UPLC-MS/MS

Wang

et al. 2015

Journal of Pharmaceutical and Biomedical Analysis

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“…Our study proved that J. pelargoniifolia roots are a source of several biologicallyactive compounds such as hordenine, which exhibited various biological activities like inhibiting melanogenesis in human melanocytes, increasing the respiratory and heart rates [31], stimulation of gastrin release, inhibition of monoamine oxidase B, and antibacterial properties [32]. Furthermore, Chrisitine et al (1979) reported that Nmethyltyramine increases blood pressure in an anaesthetized rat, relaxes guinea pig ileum, and increases both the force and rate of contraction of guinea-pig right atrium by inducing the release of noradrenaline [33].…”

Section: Biological Activitymentioning

confidence: 53%

Phytochemical and Biological Investigation of <em>Jatropha pelargoniifolia</em> Root Native to the Kingdom of Saudi Arabia

Aati¹,

Gamal²,

Kayser³

2018

Preprint

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Extensive phytochemical and chromatographic analysis of different root fractions of Jatropha pelargoniifolia Courb. (Euphorbiaceae) resulted in the isolation and identification of 22 distinct secondary metabolite compounds. Two new compounds, 6-hydroxy-8-methoxycoumarin-7-O-β-D-glycopyranoside and (3-(2-(methylamino) ethyl)-1H-indol-2-yl) methanol, were isolated and identified for the first time from a natural source. In addition, other known compounds, such as hovetricoside C and Nmethyltryptamine were isolated from Euphorbiaceae, and hordenine, Nmethyltyramine, their salts, cynaroside and linarin were identified in Jatropha spp. for the first time. Some isolated metabolites, such as β-sitosterol, β-sitosterol glucoside, curcusons D and C, naringenin, apigenin, cleomiscosins B and A, spruceanol, propacin, jatrophadiketone, and uracil were previously identified in various Jatropha species. The structures of the isolated compounds were determined using different spectroscopic techniques. The anti-inflammatory, antinociceptive, antipyretic, and antioxidant activities were evaluated for some adequately available isolated compounds. Compounds showed significant antinociceptive activity compared with the standard analgesic drug indomethacin. The edema size was significantly reduced (p< 0.05-0.001) in the animals treated with low doses (5 and 10 mg/kg) of the isolated compounds compared with those treated with a high dose (100 mg/kg) of standard anti-inflammatory drug (phenylbutazone). Furthermore, all tested compounds showed a significant (p< 0.05-0.001) reduction in the rectal temperature of hyper-thermic mice.

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“…Optimum liquid chromatography conditions separate the endogenous interfering substances from the analyte and internal standard retention times as far as possible (Ma et al, ; Jin et al, ; Wang et al, 2017; Geng et al, ). We tried acetonitrile–0.1% formic acid, acetonitrile–10 mmol/L ammonium acetate (containing 0.1% formic acid), methanol–0.1% formic acid and methanol–10 mmol/L ammonium acetate (containing 0.1% formic acid), using gradient elution.…”

Section: Resultsmentioning

confidence: 99%

Pharmacokinetics and bioavailability of gelsenicine in mice by UPLC–MS/MS

Jin

et al. 2018

Biomedical Chromatography

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Gelsenicine is an indole alkaloid isolated from Gelsemium elegans Benth. In recent years, the role of G. elegans Benth preparations in anti-tumor, analgesic, dilatation and dermatological treatment has attracted attention, and it has been applied clinically, but it is easy to cause poisoning with its use. An UPLC-MS/MS method was established to determine the gelsenicine in mouse blood, and the pharmacokinetics of gelsenicine after intravenous (0.1 mg/kg) and intragastric (0.5 and 1 mg/kg) administration was studied. Deltalin was used as internal standard; a UPLC BEH C 18 column was used for chromatographic separation. The mobile phase consisted of acetonitrile and 10 mmol/L ammonium acetate (0.1% formic acid) with a gradient elution flow rate of 0.4 mL/min. Multiple reaction monitoring mode was used for quantitative analysis of gelsenicine in electrospray ionization positive interface. Proteins from mouse blood were removed by acetonitrile precipitation. A validation of this method was performed in accordance with the US Food and Drug Administration guidelines. In the concentration range of 0.05-100 ng/mL, the gelsenicine in the mouse blood was linear (r > 0.995), and the lower limit of quantification was 0.05 ng/mL. In the mouse blood, the intra-day precision RSD was <12%, the inter-day precision RSD was <15%, the accuracy ranged from 89.8 to 112.3%, the average recovery was >76.8%, and the matrix effect was between 103.7 and 108.4%, which meet the pharmacokinetic research requirements of gelsenicine. The UPLC-MS/MS method is sensitive, rapid and selective, and has been successfully applied to the pharmacokinetic study of gelsenicine in mice. The absolute bioavailability of gelsenicine is 1.13%.

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Validated UPLC–MS/MS method for determination of hordenine in rat plasma and its application to pharmacokinetic study

Cited by 52 publications

References 32 publications

Pharmacokinetics in rats and tissue distribution in mouse of berberrubine by UPLC-MS/MS

Pharmacokinetics in rats and tissue distribution in mouse of berberrubine by UPLC-MS/MS

Phytochemical and Biological Investigation of <em>Jatropha pelargoniifolia</em> Root Native to the Kingdom of Saudi Arabia

Pharmacokinetics and bioavailability of gelsenicine in mice by UPLC–MS/MS

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