Validated TLC-Image Analysis Method for Simultaneous Quantification of Curcuminoids in Curcuma longa

Phattanawasin, Panadda; Sotanaphun, Uthai; Sriphong, Lawan

doi:10.1365/s10337-008-0893-y

Cited by 21 publications

(11 citation statements)

References 10 publications

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“…For example, Photoshop 7.0 and Scion image software were chosen to determine the amount of three curcuminoid in Curcuma longa. The techniques were validated and showed to be accurate and reliable method [16][17] .…”

Section: Discussionmentioning

confidence: 99%

Pharmacognostic evaluation and chrysazin quantitation of Xyris indica flowering heads

Khuniad¹,

Thitikornpong²,

Palanuvej³

et al. 2014

Phcog J.

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Objectives: The present study aimed to establish quality specification of Xyris indica L. flowering heads. The pharmacognostic parameters were investigated. Chrysazin contents were analyzed by TLC image analysis using ImageJ software compared to TLC-densitometry. Methods: X. indica flowering heads from 15 different sources in Thailand were collected. Morphological and physicochemical parameters were characterized. Chrysazin was successively extracted and determined by TLC image analysis using ImageJ software and TLC-densitometry. Results: Macroscopic study was illustrated as whole plant drawing. The microscopic study showed fragment of corolla, seeds, pollen grain and staminode. The pharmacognostic parameters revealed that the loss on drying, total ash, acid-insoluble ash and water content should be not more than 6.90, 2.50, 0.41, and 11.12 of % dry weight respectively while water and ethanol-soluble extractive values should be not less than 6.59 and 4.03 of % dry weight respectively. TLC fingerprint revealed clearly chrysazin yellow fluorescent band at 365 nm. Chrysazin quantitation by TLC image analysis and TLC densitometry were developed and validated. Chrysazin content was 0.022 ± 0.001 % dry weight by both methods. There was no statistically significantly difference between these methods. Conclusion: This study provided pharmacognostic specification and chrysazin content of X. indica flowering heads that can be used for basic quality control and standardization of plant material. TLC image analysis using ImageJ software showed reliable and convenient for analysis of chrysazin content in this crude drug.

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Section: Discussionmentioning

confidence: 99%

Pharmacognostic evaluation and chrysazin quantitation of Xyris indica flowering heads

Khuniad¹,

Thitikornpong²,

Palanuvej³

et al. 2014

Phcog J.

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“…High performance thin layer chromatography (HP-TLC) silica gel plates with methanol: chloroform mobile phase could be used for the evaluation of curcuminoids in benzene extracts of Curcuma longa germplasm and quantitation through densitometry at 425 nm. Phattanawasin et al (2009) reported methanol extraction prior to TLC with detection through imaging analysis [97]. RP-TLC using THF: acetonitrile: oxalic acid for quantitation by scanning densitometry can be examined turmeric oleoresin in wide range of food stuffs.…”

Section: Curcumin (E100)mentioning

confidence: 99%

A review on analytical methods for the determination of natural colorants (green) in food commodities and beverages

2020

Biointerface Res Appl Chem

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A wide variety of natural colorants are generally used for coloring food stuffs to make them more attractive, informative and reinstate the original color lost during the production process. However, in many countries the usage of natural colorants is limited due to their low stability. Green is one of the most important secondary colors as far as consumer goods are concerned due to looking fresh and natural. Chlorophyll and its metal derivatives like copper or zinc chlorophylls have been used as green colorant in food and pharmaceuticals. The regulatory authorities and food analysts have adopted precise analytical methods in assessing quality and safety of food products. These techniques mainly deal with determination of quality, quantity, colorant stability, safety and utility limits of colorants in food stuffs. These aspects are sensitively regulated by regulatory authorities as well as domestic and food suppliers. In this review, we discuss various extraction methods that are executed for extracting the chlorophyll and metallochlorophyllins from complex food commodities and beverages. In addition, different extraction methods used for food commodities have been presented. Exclusively the present manuscript reviews various analytical techniques used for the analysis of Na-Cu-Chlorophyllin (Na-Cu-Chl) employed in food products as food colorant.

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“…Several methods have been developed for authentication of C. longa including high-performance liquid chromatography [9,10], thin layer chromatography [11], high-performance thin layer chromatography [12], ultra-performance liquid chromatography [13], and capillary electrophoresis [14] which are aimed to determine the curcuminoids content. However, these methods are destructive, are not simple in sample preparation (too laborious), are time-consuming, and require more chemicals.…”

Section: Introductionmentioning

confidence: 99%

Authentication of Turmeric Using Proton-Nuclear Magnetic Resonance Spectroscopy and Multivariate Analysis

2018

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Objective: The objective of this study was to apply 1H-NMR spectroscopy-based metabolite fingerprinting in combination with multivariate analysis for authentication of turmeric (Curcuma longa) from C. heyneana and C. manga.Methods: Partial least square-discriminant analysis (PLS-DA) and orthogonal projections to latent structures-discriminant analysis (OPLS-DA) were used for differentiation of authentic and adulterated C. longa with C. manga and C. heyneana. The variables used were peaks with certain chemical shifts at optimized 1H-NMR spectra of authentic and adulterated C. longa.Results: All of the authentic C. longa samples were clearly separated from the adulterated ones. The multivariate calibration of partial least square (PLS) was successfully applied to predict of adulterants in C. longa. The lower RMSEC (root mean square error of calibration) values, 0.94% for adulterated C. longa with C. heyneana and 1.37% for adulterated C. longa with C. manga, and the lower RMSEP (root mean square error of prediction) values, 0.83% for adulterated C. longa with C. heyneana and 1.34% for adulterated C. longa with C. manga indicated the good of accuracy and precision of the calibration models.Conclusion: The combination of 1H-NMR spectroscopy and chemometrics of multivariate analysis PLS-DA, OPLS-DA, and PLS proves an adequate technique for authentication of turmeric.

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Validated TLC-Image Analysis Method for Simultaneous Quantification of Curcuminoids in Curcuma longa

Cited by 21 publications

References 10 publications

Pharmacognostic evaluation and chrysazin quantitation of Xyris indica flowering heads

Pharmacognostic evaluation and chrysazin quantitation of Xyris indica flowering heads

A review on analytical methods for the determination of natural colorants (green) in food commodities and beverages

Authentication of Turmeric Using Proton-Nuclear Magnetic Resonance Spectroscopy and Multivariate Analysis

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