Validated thin-layer chromatographic–densitometric and high-performance liquid chromatographic methods for the simultaneous determination of alfuzosin and tadalafil in pharmaceutical products

Surati, Jasmina; Kalesh, Kapila; Akbari, Ashok; Patel, Sagar; Shah, Ketan; Solanki, Divya

doi:10.1007/s00764-023-00240-5

Cited by 1 publication

(2 citation statements)

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“…Furthermore, the results in Table 3 demonstrate that the employed methods were used to selectively quantify ALF and TAD in pharmaceutical tablets without interference from excipients, as proven by the results of standard addition technique. The t-test and F-test were used to do a statistical comparison between the outcome data gained by the applied methods and those obtained by the previously published method [ 12 ]. As indicated in Table 3 , the data obtained demonstrate that no significant differences were discovered.…”

Section: Resultsmentioning

confidence: 99%

“…ALF and TAD fixed-dose combination tablets have been formulated. Only chromatographic [ 12 ] and synchronous spectrofluorometric [ 13 ] approaches for simultaneous detection of ALF and TAD were found in the literature. So far, no spectrophotometric approach for simultaneously determining ALF and TAD in their combination has been published.…”

Section: Introductionmentioning

confidence: 99%

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Utilization of absorbance subtraction and ratio difference green spectrophotometric methods for the quantification of alfuzosin hydrochloride and tadalafil in their binary mixture

Alqahtani,

Ramzy

2024

BMC Chemistry

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Alfuzosin hydrochloride and tadalafil fixed-dose combination tablets were recently formulated for the treatment of individuals with lower urinary tract symptoms caused by benign prostatic hyperplasia. Herein, the first spectrophotometric methods for quantitative analysis of alfuzosin hydrochloride and tadalafil in their binary mixture were established. The spectral overlapping of alfuzosin hydrochloride and tadalafil made direct simultaneous analysis unfeasible. Therefore, two mathematical methods were used to solve these overlapping spectra: absorbance subtraction and ratio difference. The absorbance subtraction method manipulates the zero absorption spectra of the studied drugs at the isoabsorptive point (272 nm) and uses the absorbance factor of pure ALF to calculate the absorbance of the studied drugs in the mixture at the isoabsorptive point. The ratio spectra method, on the other hand, manipulates the ratio spectra of the studied drugs, which are obtained by dividing each drug’s zero absorption spectra by a divisor spectrum from the second drug. The ratio amplitude difference between 251 nm and 211 nm was directly proportional to alfuzosin hydrochloride, whereas between 292 nm and 222 nm it was directly proportional to tadalafil. The methods used were verified in accordance with the recommendations of the ICH and demonstrated adequate linear regression in working ranges of 1–15 µg/mL for alfuzosin hydrochloride and 3–40 µg/mL for tadalafil. The methods were accurate, precise, and selectively employed to quantify alfuzosin hydrochloride and tadalafil in their combined tablets.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%