Validated single urinary assay designed for exposomic multi-class biomarkers of common environmental exposures

Jagani, Ravikumar; Pulivarthi, Divya; Patel, Dhaval; Wright, Rosalind J.; Wright, Robert O.; Arora, Manish; Wolff, Mary S.; Andra, Syam S.

doi:10.1007/s00216-022-04159-4

Cited by 7 publications

(2 citation statements)

References 73 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Multi-class analyses represent an exciting and cost-effective strategy. One of these methods successfully determined 50 analytes, including pesticides and compounds from different applications, using SPE with OASIS HLB followed by three injections on UPLC-ESI-TQ utilizing different columns (Betasil C18, Hypersil Gold AQ, and Synergi Polar RP), highlighting the potential of this reported approach ( 124 ).…”

Section: Methods and Techniquesmentioning

confidence: 99%

“…Subsequently, multi-residue approaches for analytes from the same chemical group were introduced (119, 120). In recent years, strategies for determining pesticides from several classes have been assessed, and in some cases, contaminants from consumer products have been evaluated in the same analytical run, independently of employing Triple Quadrupole, Time of Flight, or Orbitrap spectrometers (120)(121)(122)(123)(124)(125).…”

Section: Lc-ms/msmentioning

confidence: 99%

See 1 more Smart Citation

Determination of pesticide residues in urine by chromatography-mass spectrometry: methods and applications

Birolli,

Lanças,

dos Santos Neto

et al. 2024

Front. Public Health

View full text Add to dashboard Cite

IntroductionPollution has emerged as a significant threat to humanity, necessitating a thorough evaluation of its impacts. As a result, various methods for human biomonitoring have been proposed as vital tools for assessing, managing, and mitigating exposure risks. Among these methods, urine stands out as the most commonly analyzed biological sample and the primary matrix for biomonitoring studies.ObjectivesThis review concentrates on exploring the literature concerning residual pesticide determination in urine, utilizing liquid and gas chromatography coupled with mass spectrometry, and its practical applications.MethodThe examination focused on methods developed since 2010. Additionally, applications reported between 2015 and 2022 were thoroughly reviewed, utilizing Web of Science as a primary resource.SynthesisRecent advancements in chromatography-mass spectrometry technology have significantly enhanced the development of multi-residue methods. These determinations are now capable of simultaneously detecting numerous pesticide residues from various chemical and use classes. Furthermore, these methods encompass analytes from a variety of environmental contaminants, offering a comprehensive approach to biomonitoring. These methodologies have been employed across diverse perspectives, including toxicological studies, assessing pesticide exposure in the general population, occupational exposure among farmers, pest control workers, horticulturists, and florists, as well as investigating consequences during pregnancy and childhood, neurodevelopmental impacts, and reproductive disorders.Future directionsSuch strategies were essential in examining the health risks associated with exposure to complex mixtures, including pesticides and other relevant compounds, thereby painting a broader and more accurate picture of human exposure. Moreover, the implementation of integrated strategies, involving international research initiatives and biomonitoring programs, is crucial to optimize resource utilization, enhancing efficiency in health risk assessment.

show abstract

Section: Methods and Techniquesmentioning

confidence: 99%

Section: Lc-ms/msmentioning

confidence: 99%

Determination of pesticide residues in urine by chromatography-mass spectrometry: methods and applications

Birolli,

Lanças,

dos Santos Neto

et al. 2024

Front. Public Health

View full text Add to dashboard Cite

show abstract

Scaling up a targeted exposome LC-MS/MS biomonitoring method by incorporating veterinary drugs and pesticides

Hossain,

Feuerstein,

et al. 2024

Anal Bioanal Chem

View full text Add to dashboard Cite

Humans are exposed to a cocktail of food-related and environmental contaminants, potentially contributing to the etiology of chronic diseases. Better characterizing the “exposome” is a challenging task and requires broad human biomonitoring (HBM). Veterinary drugs (VDs)/antibiotics, widely used and regulated in food and animal production, however, are typically not yet included in exposomics workflows. Therefore, in this work, a previously established multianalyte liquid chromatography-tandem mass spectrometry (LC-MS/MS) method covering >80 diverse xenobiotics was expanded by >40 VDs/antibiotics and pesticides. It was investigated if the generic workflow allowed for the successful integration of a high number of new analytes in a proof-of-principle study. The expanded method was successfully in-house validated and specificity, matrix effects, linearity, intra- and inter-day precision, accuracy, limits of quantification, and detection were evaluated. The optimized method demonstrated satisfactory recovery (81–120%) for most of the added analytes with acceptable RSDs (<20%) at three spiking levels. The majority of VDs/antibiotics and pesticides (69%) showed matrix effects within a range of 50–140%. Moreover, sensitivity was excellent with median LODs and LOQs of 0.10 ng/mL and 0.31 ng/mL, respectively. In total, the expanded method can be used to detect and quantify more than 120 highly diverse analytes in a single analytical run. To the best of the authors’ knowledge, this work represents the first targeted biomonitoring method integrating VDs with various other classes of pollutants including plasticizers, PFAS, bisphenols, mycotoxins, and personal care products. It demonstrates the potential to expand targeted multianalyte methods towards additional groups of potentially toxic chemicals. Graphical Abstract

show abstract

Online solid phase extraction high-performance liquid chromatography – Isotope dilution – Tandem mass spectrometry quantification of organophosphate pesticides, synthetic pyrethroids, and selected herbicide metabolites in human urine

Wambua,

Roman,

Vidanage

et al. 2023

Chemosphere

View full text Add to dashboard Cite

Validated single urinary assay designed for exposomic multi-class biomarkers of common environmental exposures

Cited by 7 publications

References 73 publications

Determination of pesticide residues in urine by chromatography-mass spectrometry: methods and applications

Determination of pesticide residues in urine by chromatography-mass spectrometry: methods and applications

Scaling up a targeted exposome LC-MS/MS biomonitoring method by incorporating veterinary drugs and pesticides

Online solid phase extraction high-performance liquid chromatography – Isotope dilution – Tandem mass spectrometry quantification of organophosphate pesticides, synthetic pyrethroids, and selected herbicide metabolites in human urine

Contact Info

Product

Resources

About