Validated HPTLC method for simultaneous determination of shanzhiside methyl ester and barlerin inBarleria prionitis

Abstract

Aerial parts of Barleria prionitis Linn. (Acanthaceae) have shown anti-respiratory syncytial virus, anti-arthritic, anti-inflammatory, hepatoprotective, anti-stress, and immunorestorative activities. The two iridoid glycosides, shanzhiside methyl ester (SME) and barlerin, are the active principles of B. prionitis (BP). For the quality control of BP, rapid methods of quantitation are desirable. Therefore, a simple and reproducible high-performance thin-layer chromatography (HPTLC) method for the simultaneous quantification of SME and barlerin in BP was validated. The marker compounds were isolated, purified, and authenticated by spectral analysis. Precoated silica gel 60 F 254 TLC plates were used as a stationary phase and chloroform-methanol in the proportion of 80:20 (v/v) as the mobile phase. The method was validated in terms of linearity, precision, robustness, accuracy, and limits of detection and quantification. Quantification was performed in the absorbance mode at a wavelength of 240 nm using Deuterium lamp. The linearity was found to be in the range of 200-1000 ng spot -1 for both SME and barlerin, with correlation coefficients of 0.9956 and 0.9932, respectively. The percent recoveries for SME and barlerin were in the range of 99.20 to 99.54%, and 98.93 to 99.19%, respectively. Limits of detection and quantification were found to be 12.95 ng and 21.688 ng, and 17.861 ng and 31.051 ng, respectively, for SME and barlerin. The content (% w/w) of SME and barlerin was found to be 4.91 and 4.69, respectively, in methanol extract of BP. Statistical analysis of the data showed that the proposed method is simple, sensitive, precise, robust and reproducible, specific, and can be employed profitably in place of HPLC.

HPTLC Method for Shanzhiside Esters: Simultaneous Quantitative Analysis of Barlerin, Acetylbarlerin and Shanzhiside Methyl Ester in Barleria Species

“…After a bibliographic search, it was found that HPLC is the most common reported method for the analysis of shanzhiside esters [21,22,24]. To our knowledge, three reports are known where analysis of B. prionitis has been done by TLC densitometery using the solvent system chloroform-methanol (8:2, v/v) [28][29][30]. The first report was from our laboratory and this solvent system was developed for the estimation of single iridoid marker barlerin and the method was not validated.…”

A review on biological effects of Lamium album (white dead nettle) and its components

“…The most prominent compounds existing in species of Lamium genus are iridoids which usually exist as glycosides. According to the results which have been obtained from in vivo, in vitro, and clinical studies, iridoid-rich plants have anticancer, antioxidant, antibacterial, anti-viral, anti-inflammatory, antiarthritic, immunomodulatory, neuroprotective, and wound healing activities (17).…”

Comparative secondary metabolite expression in callus cultures and mother plant in Barleria prionitis L.

“…Its important active principles are shanzhiside methyl ester (SME), barlerin, and squalene apart from phenolics and avonoids. SME is an iridoid glycoside that has been reported to show various bioactivities, such as anti-in ammatory, anti-depressant, anti-diabetic, antioxidant, and neuroprotective effects (Ghule et al 2012). Some of the possible mechanisms of action of SME include inhibiting the production of pro-in ammatory cytokines, stimulating the expression of β-endorphin in spinal microglia, modulating the activity of monoamine oxidase and serotonin transporter, enhancing the insulin signaling pathway, scavenging free radicals, and protecting against neuronal damage (Ghule et al 2020;Sun et al 2022).…”

“…It has multiple industrial applications in different elds such as pharmaceuticals, cosmetics, food supplements and biofuels (Lozano-Grande et al 2018). Both SME (Ghule et al 2012) and squalene (Nidhi et al 2013) have been quanti ed and isolated in B. prionitis using techniques like HPTLC, ash chromatography and GC-MS and structure of these compounds were elucidated with the aid of extensive NMR spectral studies.…”