Validated HPLC method for determination of amlodipine in human plasma and its application to pharmacokinetic studies

Zarghi, Afshin; Foroutan, Seyed Mohsen; Shafaati, Alireza; Khoddam, Arash

doi:10.1016/j.farmac.2005.06.012

Cited by 86 publications

(51 citation statements)

References 16 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…After oral management of therapeutic doses of amlodipine, absorption produces height plasma concentrations between 6 and 12 h. Amlodipine is considerably (approximately 90%) transformed to inactive metabolites through hepatic metabolism with 10% of the determined compound and 60% of the metabolites excreted in the urine. Amlodipine is slowly and nearly absolutely absorbed from the gastrointestinal tract [6]. Peak plasma attention is reached 6-12 h following oral management.…”

Section: Introductionmentioning

confidence: 99%

Analytical Method Development and Validation of Amlodipine in Human Plasma Using Liquid Chromatography-Mass Spectrometry/Mass Spectrometry

Mohanty

et al. 2018

Asian J Pharm Clin Res

View full text Add to dashboard Cite

Objective: The objective of the present investigation was to develop a novel, simple, and economic method for the estimation of amlodipine in positive ion mode in human plasma using amlodipine maleate d4 as an internal standard. Methods:The chromatographic separation was performed on Zorbax SB, C 18 , 50 mm*4.6 mm, and 3.5 mm. The mobile phase was prepared with a mixture of 5 mm ammonium acetate in 0.1% formic acid: High performance liquid chromatographic (HPLC) grade methanol:HPLC grade acetonitrile (40:30:30) that run isocratically at the flow rate of 0.700 ml/min and run time at 2.50 min. Results:The analytical method is valid for the estimation of amlodipine, in human plasma over a range of 0.100 ng/ml-9.990 ng/ml with the detection of amlodipine m/z -409.10 (parent) and 238.00 (product), and internal standard Amlodipine Maleate d4 m/z -413.20 (parent), and 238.00 (product) in positive ion mode. The results of carryover test, matrix effect, linearity, precision and accuracy, stabilities, dilution integrity, and run size evaluation test presented in this report are within the acceptance range. Conclusion:A sensitive method for the separation and determination of amlodipine in plasma has been developed based on solid-phase extraction with disposable extraction cartridges in combination with LC and mass spectrophotometers (MS/MS).

show abstract

Section: Introductionmentioning

confidence: 99%

Analytical Method Development and Validation of Amlodipine in Human Plasma Using Liquid Chromatography-Mass Spectrometry/Mass Spectrometry

Mohanty

et al. 2018

Asian J Pharm Clin Res

View full text Add to dashboard Cite

show abstract

“…There are many methods published for determination of AMO in dosage form and in plasma. Most of these methods utilized high-performance liquid chromatography (HPLC) with UV, fluorescence and mass detections [3][4][5][6][7][8][9][10][11][12][13][14]. Some of these methods use spectrofluorometry [15,16].…”

Section: Introductionmentioning

confidence: 99%

Utilization of oxidation-reduction reaction of Folin-Ciocalteu’s phenol reagent in colorimetric determination of amlodipine in pharmaceutical dosage form

Mostafa

Abotaleb

2016

Eur. J. Chem.

View full text Add to dashboard Cite

A simple visible spectrophotometric method has been developed for determination of amlodipine in commercial tablets. Amlodipine (AMO) is 1,4-dihydropyridine derivative, this group when reacted with Folin-Ciocaluts phenol reagent in presence of 10% sodium carbonate, intense blue color was formed that exhibits maximum absorbance at wave length 745 nm. The intensity of color was found to be affected by many parameters. Optimization of the method parameters was done. The method was validated over a concentration range of 10 to 110 µg/mL. The values of each validation items were within the acceptable range for precision and accuracy. The lower limit for quantitation and lower of detection were founded to be 3.21 and 9.72 µg/mL, respectively. The proposed method was successfully applied to the quantitative determination of amlodipine in tablets without interference from tablet additives. The simplicity and accuracy of this method will allow many laboratories to determine the concentration of amlodipine in dosage form in easy way.

show abstract

“…Several analytical procedures are available for the analysis of AD in the plasma/serum, such as high-performance liquid chromatography (HPLC) with fluorescence detection (Tatar, Atamaca, 2001;Bahrami, Mirzaeei, 2004); ultraviolet (UV) detection (Yeung et al, 1991;Coseberg, Carson, 1997;Patel et al, 1998;Johansen, Genner, 2003;Zarghi et al, 2005); amperometric detection (Shirmooka et al, 1989;Josefsson et al, 1995); highperformance thin layer chromatography method (Pandya 720 et al, 1995); liquid chromatography tandem mass spectrometry (LC-MS-MS) method (Yasud et al, 1996;Chen et al, 2001;Massaroti et al, 2005;Nirogi et al, 2006;Ma et al, 2007;Sirikatitham et al, 2008); gas chromatography (GC) method (Bresford et al, 1987;Scharpf et al, 1994;Monkman et al, 1996); GC-MS method (Feng et al, 1998) and electrochemical analysis (Gazy, 2004). Generally, AD in the plasma has been analyzed by HPLC (-MS) and GC methods.…”

Section: Introductionmentioning

confidence: 99%

Spectrofluorimetric determination of amlodipine in human plasma without derivatization

Kadıoğlu

Öztürk

2012

Braz. J. Pharm. Sci.

View full text Add to dashboard Cite

A rapid and sensitive spectrofluorimetric method was developed for the determination of amlodipine (AD), a calcium channel blocker, in the plasma. The type of solvent, the wavelength range, and the range of AD concentration were selected to optimize the experimental conditions. The calibration curves were linear (r 2 ≥0.997) in the concentration range of 0.1-12.5 ppm of AD. The limit of quantitation and limit of detection values for the method for plasma samples were 0.1 ppm and 0.07 ppm, respectively. The precision calculated as the relative standard deviation was less than 3.5%, and the accuracy (relative error) was better than 5.5% (n=6). The method developed in this study can be directly and easily applied for the determination of AD in the plasma without derivatization in plasma.Uniterms: Amlodipine/determination. Spectrofluorimetric method/validation. Human plasma.Método espectrofluorometrico rápido e sensível é descrito para a determinação de anlodipina (AD), um bloqueador de canais de cálcio, em amostras de plasma. O tipo de solvente, a faixa de comprimento de onda e a faixa de concentração foram escolhidas a fim de otimizar as condições experimentais. As curvas de calibração foram lineares (r ≥ 0,997) na faixa de concentração de 0,1-12,5 ppm de AD. Os valores LoQ e LoD do método para amostras de plasma foram 0,1 ppm e 0,07 ppm, respectivamente. A precisão calculada como desvio padrão relativo (RSD) foi menor do que 3,5% e a precisão (erro relativo) foi melhor do que 5,5% (n=6). O método desenvolvido neste estudo pode ser fácil e diretamente aplicado para a determinação de AD sem derivatização no plasma.Unitermos: Anlodipina/determinação. Método espectrofluorimétrico/validação. Plasma humano.

show abstract

Validated HPLC method for determination of amlodipine in human plasma and its application to pharmacokinetic studies

Cited by 86 publications

References 16 publications

Analytical Method Development and Validation of Amlodipine in Human Plasma Using Liquid Chromatography-Mass Spectrometry/Mass Spectrometry

Analytical Method Development and Validation of Amlodipine in Human Plasma Using Liquid Chromatography-Mass Spectrometry/Mass Spectrometry

Utilization of oxidation-reduction reaction of Folin-Ciocalteu’s phenol reagent in colorimetric determination of amlodipine in pharmaceutical dosage form

Spectrofluorimetric determination of amlodipine in human plasma without derivatization

Contact Info

Product

Resources

About