Validated High-Performance Liquid Chromatographic (HPLC) Method for the Simultaneous Quantification of 2,3-Butanediol, Glycerol, Acetoin, Ethanol, and Phosphate in Microbial Cultivations

Souza, Bruna Campos de; Bossardi, Flávia Frozza; Furlan, Greice Ribeiro; Folle, Analia Borges; Reginatto, Caroline; Polidoro, Tomás Augusto; Carra, Sabrina; Silveira, Maurício Moura da; Malvessi, Eloane

doi:10.1080/00032719.2020.1869754

Cited by 10 publications

(7 citation statements)

References 45 publications

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“…So far, the determination of phosphate methods include high-performance liquid chromatography (HPLC), 3 flow injection analysis (FIA), 4 and so on. 5,6 However, there exist many limitations in these methods, such as intricate operation steps, lengthy detection cycles, and expensive detection costs, which limit their practical applications.…”

Section: Introductionmentioning

confidence: 99%

Highly sensitive colorimetric detection and effective adsorption of phosphate based on MOF-808(Zr/Ce)

Zhai

Hou

et al. 2022

New J. Chem.

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Section: Introductionmentioning

confidence: 99%

Highly sensitive colorimetric detection and effective adsorption of phosphate based on MOF-808(Zr/Ce)

Zhai

Hou

et al. 2022

New J. Chem.

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“…Organic acids (succinic, acetic, and lactic acids), acetoin, and 2,3-butanediol were detected by liquid chromatography on an Agilent 1290 Infinity (Agilent Technologies, Waldbronn, Germany) instrument. The column model was an Aminex HPX-87H (Bio-Rad), injection volume was 20 μL, mobile phase was 5 mM H 2 SO 4 , flow rate was 0.6 mL/min, column temperature was 60 °C, and a refractive index detector was used (Souza et al 2021 ). TTMP was determined as per Peng et al ( 2020 ).…”

Section: Methodsmentioning

confidence: 99%

Production of acetoin and its derivative tetramethylpyrazine from okara hydrolysate with Bacillus subtilis

Liu

Guo

et al. 2023

AMB Expr

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Okara, a renewable biomass resource, is a promising fermentative raw material for the bio-production of value-added chemicals due to its abundance and low-costs. we developed a process for the enzymatic hydrolysis of okara, and then engineered Bacillus subtilis to utilize mixed sugars to produce acetoin in okara hydrolysis without the addition of a supplemental nitrogen source. Okara was initially hydrolyzed with cellulase, β-glucosidase, and pectinase to obtain okara hydrolysate containing mixed sugars (32.78 ± 0.23 g/L glucose, 1.43 ± 0.064 g/L arabinose, 7.74 ± 0.11 g/L galactose) and amino acids. In this study, Bacillus subtilis 168 was used as the acetoin-producing strain, and the key genes bdhA and acoA of the acetoin catabolism pathway were knocked out to improve the fermentation yield of acetoin. In order to utilize the galactose in the hydrolysate, the recombinant strain BS03 (Bacillus subtilis168∆bdhA∆acoA) was used to overexpress the arabinose transporter-encoding gene (araE) drive heterologous expression of the Leloir pathway gene (galKTE). The corn dry powder concentration was optimized to 29 g/L in the reducing sugar okara hydrolysate. The results show that the recombinant bacterium BS03 could still synthesize 11.79 g/L acetoin without using corn dry powder as a nitrogen source. Finally, using okara enzymatic hydrolysate as the carbon and nitrogen source, 11.11 g/L and 29.7 g/L acetoin were obtained by batch fermentation and fed-batch fermentation, respectively, which was further converted to 5.33 g/L and 13.37 g/L tetramethylpyrazine (TTMP) by reaction with an ammonium salt.

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“…The feed and permeate streams of hydrophilic pervaporation were analyzed by HPLC, following the procedures described by Souza et al [ 47 ]. We used a Shimadzu LC-20AD HPLC system (Shimadzu Corporation, Kyoto, Japan).…”

Section: Methodsmentioning

confidence: 99%

Non-Supported and PET-Supported Chitosan Membranes for Pervaporation: Production, Characterization, and Performance

et al. 2022

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The objective of this study was to develop non-supported and PET-supported chitosan membranes that were cross-linked with glutaraldehyde, then evaluate their physical–chemical, morphological, and mechanical properties, and evaluate their performance in the separation of ethanol/water and limonene/linalool synthetic mixtures by hydrophilic and target-organophilic pervaporation, respectively. The presence of a PET layer did not affect most of the physical-chemical parameters of the membranes, but the mechanical properties were enhanced, especially the Young modulus (76 MPa to 398 MPa), tensile strength (16 MPa to 27 MPa), and elongation at break (7% to 26%), rendering the supported membrane more resistant. Regarding the pervaporation tests, no permeate was obtained in target-organophilic pervaporation tests, regardless of membrane type. The support layer influenced the hydrophilic pervaporation parameters of the supported membrane, especially in reducing transmembrane flux (0.397 kg∙m−2∙h−1 to 0.121 kg∙m−2∙h−1) and increasing membrane selectivity (611 to 1974). However, the pervaporation separation index has not differed between membranes (228 for the non-supported and 218 for the PET-supported membrane), indicating that, overall, both membranes had a similar performance. Thus, the applicability of each membrane is linked to specific applications that require a more resistant membrane, greater transmembrane fluxes, and higher selectivity.

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Validated High-Performance Liquid Chromatographic (HPLC) Method for the Simultaneous Quantification of 2,3-Butanediol, Glycerol, Acetoin, Ethanol, and Phosphate in Microbial Cultivations

Cited by 10 publications

References 45 publications

Highly sensitive colorimetric detection and effective adsorption of phosphate based on MOF-808(Zr/Ce)

Highly sensitive colorimetric detection and effective adsorption of phosphate based on MOF-808(Zr/Ce)

Production of acetoin and its derivative tetramethylpyrazine from okara hydrolysate with Bacillus subtilis

Non-Supported and PET-Supported Chitosan Membranes for Pervaporation: Production, Characterization, and Performance

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