Valdecoxib Recovers the Lipid Composition, Order and Dynamics in Colon Cancer Cell Lines Independent of COX-2 Expression: An ATR-FTIR Spectroscopy Study

Genç, Aysun; Gök, Seher; Banerjee, Sreeparna; Severcan, Feride

doi:10.1177/0003702816654164

Cited by 16 publications

(10 citation statements)

References 72 publications

(96 reference statements)

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“…About this, Ognjanović et al reported an increase in lipid oxidation activity related to KI; in our results, we found that CH 2 /CH 3 ratio decreased in the ISS group, and the ratio A1746/A1457 related to lipid peroxidation increased significantly in this group at day 8 (Figures 6(d) and 6(e)). These ratio changes indicate an increment in lipid saturation, corroborating the lipid oxidation as well as the accumulation of long chain length lipids reported by Yan et al and Genç et al [43, 51]. Furthermore, these events are related to apoptosis that also is associated with different membrane changes, such as phosphatidylserine exposure, membrane blebbing, and vesicle formation.…”

Section: Discussionsupporting

confidence: 86%

Nephroprotective Effect of Embryonic Stem Cells Reducing Lipid Peroxidation in Kidney Injury Induced by Cisplatin

Mata-Miranda

Barquero

Martínez-Cuazitl

et al. 2019

Oxidative Medicine and Cellular Longevity

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Introduction. The acute kidney injury (AKI) is characterized by a sudden glomerular filtration reduction. Renal or intrinsic causes of AKI include nephrotoxicity induced by exogenous agents like cisplatin, which causes oxidative stress altering the biochemical process and leading to apoptosis. Therefore, this research is aimed at analyzing the embryonic stem cells (ESC) nephroprotective effect in AKI induced by cisplatin, employing genetic, phenotypic, and microspectroscopic techniques. Methods. Thirty mice were randomly divided into three groups (n=10): the healthy, isotonic salt solution (ISS), and mouse embryonic stem cells (mESC) groups. The ISS and mESC groups were subjected to AKI using cisplatin; 24 h post-AKI received an intraperitoneal injection of ISS or 1×106 mESC, respectively. At days 4 and 8 post-AKI, five mice of each group were sacrificed to analyze the histopathological, genetic (PDK4 and HO-1), protein (p53), and vibrational microspectroscopic changes. Results. Histopathologically, interstitial nephritis and acute tubular necrosis were observed; however, the mESC group showed a more preserved microarchitecture with high cellularity. Additionally, the PDK4 and HO-1 gene expression only increased in the ISS group on day 4 post-AKI. Likewise, p53 was more immunoexpressed at day 8 post-AKI in the ISS group. About biomolecular analysis by microspectroscopy, bands associated with lipids, proteins, and nucleic acids were evidenced. Besides, ratios related to membrane function (protein/lipid), unsaturated lipid content (olefinic/total lipid, olefinic/total CH2, and CH2/CH3), and lipid peroxidation demonstrated oxidative stress induction and lipid peroxidation increase mainly in the ISS group. Finally, the principal component analysis discriminated against each group; nonetheless, some data of the healthy and mESC groups at day 8 were correlated. Conclusions. The mESC implant diminishes cisplatin nephrotoxicity, once the protective effect in the reduction of lipid peroxidation was demonstrated, reflecting a functional and histological restoration.

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Section: Discussionsupporting

confidence: 86%

Nephroprotective Effect of Embryonic Stem Cells Reducing Lipid Peroxidation in Kidney Injury Induced by Cisplatin

Mata-Miranda

Barquero

Martínez-Cuazitl

et al. 2019

Oxidative Medicine and Cellular Longevity

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“…To determine any alterations in nucleic acid content induced by low doses of DMSO, we analysed the PO 2 antisymmetric band from the absorbance spectrum located in between 1242–1238 cm −1 that is assigned to total nucleic acids 12 . Additionally, the band located at 915 cm −1 and emerging from Ribose ring vibrations of total RNA and DNA 13 , 14 was analysed from the second derivative spectra. The intensities (%) of the PO 2 antisymmetric band are summarized in Fig.…”

Section: Resultsmentioning

confidence: 99%

“…The reaction of free radicals with the olefinic bonds of polyunsaturated lipids results in lipid peroxidation, which in turn causes the loss of unsaturation in the lipid structure together with the breakdown of longer chains into smaller lipid acyl chains 14 . The olefinic band located at 3009 cm −1 in the lipid region of IR spectra assigned to unsaturated lipids was analysed to measure the level of unsaturation and lipid peroxidation end-products as a result of DMSO treatment.…”

Section: Resultsmentioning

confidence: 99%

“…An increase in intensity of this unsaturation marker band 13 is related to elevated concentrations of unsaturated acyl chains of lipids and loss of lipid-peroxidation end-products 75 – 77 . Specifically, intensities (%) of olefinic band and the ratio of olefinic to total lipids (olefinic/total lipid) were analysed to monitor the acyl chain unsaturation level of the phospholipids 14 (Fig. 7C,D ).…”

Section: Resultsmentioning

confidence: 99%

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Low dose dimethyl sulfoxide driven gross molecular changes have the potential to interfere with various cellular processes

Tunçer

Gurbanov

Sheraj

et al. 2018

Sci Rep

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Dimethyl sulfoxide (DMSO) is a small molecule with polar, aprotic and amphiphilic properties. It serves as a solvent for many polar and nonpolar molecules and continues to be one of the most used solvents (vehicle) in medical applications and scientific research. To better understand the cellular effects of DMSO within the concentration range commonly used as a vehicle (0.1–1.5%, v/v) for cellular treatments, we applied Attenuated Total Reflectance (ATR) Fourier Transform Infrared (FT-IR) spectroscopy to DMSO treated and untreated epithelial colon cancer cells. Both unsupervised (Principal Component Analysis-PCA) and supervised (Linear Discriminant Analysis-LDA) pattern recognition/modelling algorithms applied to the IR data revealed total segregation and prominent differences between DMSO treated and untreated cells at whole, lipid and nucleic acid regions. Several of these data were supported by other independent techniques. Further IR data analyses of macromolecular profile indicated comprehensive alterations especially in proteins and nucleic acids. Protein secondary structure analysis showed predominance of β-sheet over α-helix in DMSO treated cells. We also observed for the first time, a reduction in nucleic acid level upon DMSO treatment accompanied by the formation of Z-DNA. Molecular docking and binding free energy studies indicated a stabilization of Z-DNA in the presence of DMSO. This alternate DNA form may be related with the specific actions of DMSO on gene expression, differentiation, and epigenetic alterations. Using analytical tools combined with molecular and cellular biology techniques, our data indicate that even at very low concentrations, DMSO induces a number of changes in all macromolecules, which may affect experimental outcomes where DMSO is used as a solvent.

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“…Altered the lipid composition of cell membrane which resulted in anti-inflammatory activity in cancer cells 38 …”

Section: Resultsmentioning

confidence: 99%

Crocodylus porosus: a potential source of anticancer moleculesCrocodylus porosus: a potential source of anticancer molecules

et al. 2020

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BackgroundCancer remains a global threat resulting in significant morbidity and mortality despite advances in therapeutic interventions, suggesting urgency for identification of anticancer agents. Crocodiles thrive in polluted habitat, feed on germ-infested meat, are exposed to carcinogenic heavy metals, are the very few species to survive the catastrophic Cretaceous–Paleogene extinction event, yet have a prolonged lifespan and rarely been reported to develop cancer. Therefore, we hypothesised that animals living in polluted environments such as crocodiles possess anticancer molecules/mechanisms.MethodsCrocodylus porosus was procured, blood collected, dissected and lysates prepared from internal organs. Organ lysates and sera were tested for growth inhibition, cytotoxic effects and cell survival against HeLa, PC3 and MCF7 cells and subjected to liquid chromatography mass spectrometry. RNA transcriptome analysis and differential gene analysis were performed using Galaxy Bioinformatics.ResultsSera exhibited potent growth inhibition and cytotoxic effects against cancer cells. 80 molecules were detected from C. porosus and 19 molecules were putatively identified. Additionally, more than 100 potential anticancer peptides were identified from sera using bioinformatics based on peptide amino acid composition, binary profile, dipeptide composition and pseudo-amino acid composition. Following transcriptome analysis, 14 genes in treated HeLa cells, 51 genes in treated MCF7 cells and 2 genes in treated PC3 cells, were found to be expressed, compared with untreated controls.ConclusionAnimals residing in polluted milieus are an unexploited source for prospective pharmaceutical drugs, and could lead to identification of novel antitumour compound(s) and/or further understanding of the mechanisms of cancer resistance.

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Valdecoxib Recovers the Lipid Composition, Order and Dynamics in Colon Cancer Cell Lines Independent of COX-2 Expression: An ATR-FTIR Spectroscopy Study

Cited by 16 publications

References 72 publications

Nephroprotective Effect of Embryonic Stem Cells Reducing Lipid Peroxidation in Kidney Injury Induced by Cisplatin

Nephroprotective Effect of Embryonic Stem Cells Reducing Lipid Peroxidation in Kidney Injury Induced by Cisplatin

Low dose dimethyl sulfoxide driven gross molecular changes have the potential to interfere with various cellular processes

Crocodylus porosus: a potential source of anticancer moleculesCrocodylus porosus: a potential source of anticancer molecules

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