Vagus Nerve Electroneurogram-Based Detection of Acute Pentylenetetrazol Induced Seizures in Rats

Stumpp, Lars; Smets, Hugo; Vespa, Simone; Cury, Joaquin; Doguet, Pascal; Delbeke, Jean; Nonclercq, Antoine; Tahry, Riëm El

doi:10.1142/s0129065721500246

Cited by 9 publications

(9 citation statements)

References 48 publications

(62 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In the context of clinical applications, while techniques relying on scattering changes seem more appropriate than those based on birefringence changes for sciatic nerves, the latter method could still be promising for nerves containing less myelinated axons. It is the case, for instance, for the vagus nerve, showing a specific clinical interest in epilepsy [64]. To which extend polarization techniques could be implemented for monitoring neural activity in vagus nerves remains an open question and requires further investigations.…”

Section: Discussionmentioning

confidence: 99%

Optical birefringence changes in myelinated and unmyelinated nerves: A comparative study

Cury

Smets

Bouzin

et al. 2022

Journal of Biophotonics

Self Cite

View full text Add to dashboard Cite

The measurement of birefringence variations related to nerve activity is a promising label-free technique for sensing compound neural action potentials (CNAPs). While widely applied in crustaceans, little is known about its efficiency on mammal peripheral nerves. In this work, birefringence recordings to detect CNAPs, and Stokes parameters measurements were performed in rat and lobster nerves. While single-trial detection of nerve activity in crustaceans was achieved successfully, no optical signal was detected in rats, even after extensive signal filtering and averaging. The Stokes parameters showed that a high degree of polarization of light is maintained in lobster sample, whereas an almost complete light depolarization occurs in rat nerve. Our results indicate that depolarization itself is not sufficient to explain the absence of birefringence signals in rats. We hypothesize that this absence comes from the myelin sheets, which constraint the birefringence changes to only take place at the nodes of Ranvier.

show abstract

Section: Discussionmentioning

confidence: 99%

Optical birefringence changes in myelinated and unmyelinated nerves: A comparative study

Cury

Smets

Bouzin

et al. 2022

Journal of Biophotonics

Self Cite

View full text Add to dashboard Cite

show abstract

“…Diverse drugs such as pilocarpine or kainic acid have been used as chemoconvulsants in animal models of epilepsy for testing new ACD candidates [ 50 ]. One of the gold-standard models for the screening of new ACDs involves the chemical induction of seizures with pentylenetetrazol (PTZ), which is capable of generating acute seizures [ 51 , 52 , 53 ]. The mechanism of action of PTZ consists of noncompetitive blocking of GABA A receptors through inhibition of the chloride channel associated with the receptor complex.…”

Section: Treatment Of Seizures and Screening Of New Drugsmentioning

confidence: 99%

The GABAergic System and Endocannabinoids in Epilepsy and Seizures: What Can We Expect from Plant Oils?

et al. 2022

View full text Add to dashboard Cite

Seizures and epilepsy are some of the most common serious neurological disorders, with approximately 80% of patients living in developing/underdeveloped countries. However, about one in three patients do not respond to currently available pharmacological treatments, indicating the need for research into new anticonvulsant drugs (ACDs). The GABAergic system is the main inhibitory system of the brain and has a central role in seizures and the screening of new ACD candidates. It has been demonstrated that the action of agents on endocannabinoid receptors modulates the balance between excitatory and inhibitory neurotransmitters; however, studies on the anticonvulsant properties of endocannabinoids from plant oils are relatively scarce. The Amazon region is an important source of plant oils that can be used for the synthesis of new fatty acid amides, which are compounds analogous to endocannabinoids. The synthesis of such compounds represents an important approach for the development of new anticonvulsant therapies.

show abstract

“…(b) During the chronic experiment, electrodes impedances were controlled. (c) We used a previously validated spike detection algorithm [16] to quantify spike activity in the VENG signal acquired chronically. The shape of the spikes was also visually validated.…”

Section: Chronic Recordingmentioning

confidence: 99%

“…The VENG was software filtered between 300 and 3000 Hz (2nd order Butterworth). The number of spikes per second obtained for each hour of the recording was computed using a spike detection algorithm adapted from [16,18,23,24]. Spike are detected based on their waveform (template matching), with templates that are automatically adapted to each subject (subject-specific templates) and based on specific features of the waveform (spike slope, curvature, and duration).…”

Section: Veng Processingmentioning

confidence: 99%

“…Recently, the chronic recording of the vagus nerve has been developed and several studies reported the feasibility of such recordings [11][12][13][14]. Chronic continuous analysis of the vagus nerve activity could contribute to the understanding of the role of the vagus nerve in the circadian oscillations in general, and could be of special interest in the frame of epilepsy [15,16]. In this paper, we present for the first time the 24 h patterns of spontaneous vagus nerve activity found in continuous chronic vagus nerve recordings performed in four rats, which was previously reported only by indirect measurement.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Chronic recording of the vagus nerve to analyze modulations by the light–dark cycle

Smets¹,

Stumpp²,

Chávez³

et al. 2022

J. Neural Eng.

Self Cite

View full text Add to dashboard Cite

Objective. The vagus nerve is considered to play a key role in the circadian rhythm. Chronic continuous analysis of the vagus nerve activity could contribute to a better understanding of the role of the vagus nerve in light-dark modulations. This paper presents a continuous analysis of spontaneous vagus nerve activity performed in four rats. Approach. We analyzed the vagus electroneurogram (VENG) and electroencephalogram (EEG) over a recording period of 28 days. Spike activity and heart rate estimation were derived from the VENG, and slow-wave activity was derived from the EEG. The presence of repetitive patterns was investigated with periodograms, cosinor fitting, autocorrelation, and statistical tests. The light-dark variations derived from the VENG spikes were compared with EEG slow waves, an established metric in circadian studies. Results. Our results demonstrate that light-dark variations can be detected in long-term vagus nerve activity monitoring. A recording period of about seven days is required to characterize accurately the VENG light-dark variations. Significance. As a major outcome of this study, vagus nerve recordings hold the promise to help understand circadian regulation.

show abstract

Vagus Nerve Electroneurogram-Based Detection of Acute Pentylenetetrazol Induced Seizures in Rats

Cited by 9 publications

References 48 publications

Optical birefringence changes in myelinated and unmyelinated nerves: A comparative study

Optical birefringence changes in myelinated and unmyelinated nerves: A comparative study

The GABAergic System and Endocannabinoids in Epilepsy and Seizures: What Can We Expect from Plant Oils?

Chronic recording of the vagus nerve to analyze modulations by the light–dark cycle

Contact Info

Product

Resources

About