Vaginal microbiota affects urinary tract infection risk in women with systemic lupus erythematosus: a pilot cross-sectional study from Thailand

Rotjanapan, Porpon; Jaroensukrungruang, Aunyakant; Pisitkun, Prapaporn; Ngamjanyaporn, Pintip; Manonai, Jittima; Sawaswong, Vorthon; Chanchaem, Prangwalai; Payungporn, Sunchai

doi:10.1136/lupus-2021-000551

Cited by 4 publications

(2 citation statements)

References 46 publications

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“…Veillonella abundance has been linked to several pathologies in humans. For example, it is linked urinary tract infection risk in vagina flora ( 31 ), and to caries in oral microbiota ( 32 ). Interestingly, its abundance in the airways or in the gut at one month of age is also associated with later asthma development in childhood ( 33 , 34 ).…”

Section: Discussionmentioning

confidence: 99%

Circulating microbiome analysis in patients with perioperative anaphylaxis

de Chaisemartin,

Ciocan,

Gouel-Chéron

et al. 2024

Front. Immunol.

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BackgroundPerioperative anaphylaxis is a rare and acute systemic manifestation of drug-induced hypersensitivity reactions that occurs following anesthesia induction; the two main classes of drugs responsible for these reactions being neuromuscular blocking agents (NMBA) and antibiotics. The sensitization mechanisms to the drugs are not precisely known, and few risk factors have been described. A growing body of evidence underlines a link between occurrence of allergy and microbiota composition. However, no data exist on microbiota in perioperative anaphylaxis. The aim of this study was to compare circulating microbiota richness and composition between perioperative anaphylaxis patients and matched controls.MethodsCirculating 16s rDNA was quantified and sequenced in serum samples from 20 individuals with fully characterized IgE-mediated NMBA-related anaphylaxis and 20 controls matched on sex, age, NMBA received, type of surgery and infectious status. Microbiota composition was analyzed with a published bioinformatic pipeline and links with patients clinical and biological data investigated.ResultsAnalysis of microbiota diversity showed that anaphylaxis patients seem to have a richer circulating microbiota than controls, but no major differences of composition could be detected with global diversity indexes. Pairwise comparison showed a difference in relative abundance between patients and controls for Saprospiraceae, Enterobacteriaceae, Veillonellaceae, Escherichia-Shigella, Pseudarcicella, Rhodoferax, and Lewinella. Some taxa were associated with concentrations of mast cell tryptase and specific IgE.ConclusionWe did not find a global difference in terms of microbiota composition between anaphylaxis patient and controls. However, several taxa were associated with anaphylaxis patients and with their biological data. These findings must be further confirmed in different settings to broaden our understanding of drug anaphylaxis pathophysiology and identify predisposition markers.

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Section: Discussionmentioning

confidence: 99%

Circulating microbiome analysis in patients with perioperative anaphylaxis

de Chaisemartin,

Ciocan,

Gouel-Chéron

et al. 2024

Front. Immunol.

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“…They also documented that the use of prophylactic antibiotic therapy with trimethoprim sulfamethoxazole had an impact on the vaginal microflora. A relationship like this can favor the development of pathogenic bacteria, resulting in an infection and exacerbation of SLE [ 57 ].…”

Section: Genital Microbiotamentioning

confidence: 99%

Lupus Nephritis and Dysbiosis

2023

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Lupus nephritis (LN) is one of the most common and serious complications of systemic lupus erythematosus (SLE). The risk factors for developing LN by SLE patients are not fully understood. They are considered to be a mix of genetic and environmental variables, one of them being dysbiosis, proposed recently to interfere with autoimmunity. As of yet, the relations between the human microbiome, its genetic determinants, individual variability and clinical consequences remain to be established. One of the major obstacles in studying them is the magnitude of confounders, such as diet, drugs, infections or antibiotics use. They also make comparison between the studies extremely complicated. We reviewed the available evidence for the interplay between microbiome, dysbiosis and mechanisms triggering the autoimmune responses and potentially contributing to LN development. One such mechanism is the stimulation of autoimmune responses by bacterial metabolites that can mimic autoantigens and cause antibody production. These mimicking microbial antigens seem to be a promising target for future interventions.

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Detecting and quantifying Veillonella by real-time quantitative PCR and droplet digital PCR

Ding,

Cui,

Zhang

et al. 2024

Appl Microbiol Biotechnol

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Veillonella spp. are Gram-negative opportunistic pathogens present in the respiratory, digestive, and reproductive tracts of mammals. An abnormal increase in Veillonella relative abundance in the body is closely associated with periodontitis, inflammatory bowel disease, urinary tract infections, and many other diseases. We designed a pair of primers and a probe based on the 16S rRNA gene sequences of Veillonella and conducted real-time quantitative PCR (qPCR) and droplet digital PCR (ddPCR) to quantify the abundance of Veillonella in fecal samples. These two methods were tested for specificity and sensitivity using simulated clinical samples. The sensitivity of qPCR was 100 copies/μL, allowing for the accurate detection of a wide range of Veillonella concentrations from 10 3 to 10 8 CFU/mL. The sensitivity of ddPCR was 11.3 copies/μL, only allowing for the accurate detection of Veillonella concentrations from 10 1 to 10 4 CFU/mL because of the limited number of droplets generated by ddPCR. ddPCR is therefore more suitable for the detection of low-abundance Veillonella samples. To characterize the validity of the assay system, clinical samples from children with inflammatory bowel disease were collected and analyzed, and the results were verified using isolation methods. We conclude that molecular assays targeting the 16S rRNA gene provides an important tool for the rapid diagnosis of chronic and infectious diseases caused by Veillonella and also supports the isolation and identification of Veillonella for research purposes. Key points• With suitable primer sets, the qPCR has a wider detection range than ddPCR.• ddPCR is suitable for the detection of low-abundance samples.• Methods successfully guided the isolation of Veillonella in clinical sample.

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Vaginal microbiota affects urinary tract infection risk in women with systemic lupus erythematosus: a pilot cross-sectional study from Thailand

Cited by 4 publications

References 46 publications

Circulating microbiome analysis in patients with perioperative anaphylaxis

Circulating microbiome analysis in patients with perioperative anaphylaxis

Lupus Nephritis and Dysbiosis

Detecting and quantifying Veillonella by real-time quantitative PCR and droplet digital PCR

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