Vaginal
            <i>Atopobium</i>
            is Associated with Spontaneous Abortion in the First Trimester: a Prospective Cohort Study in China

Chen, Si; Xue, Xiaomeng; Zhang, Yingxuan; Zhang, Huimin; Huang, Xuge; Chen, Xiaofeng; Deng, Gaopi; Luo, Songping; Gao, Jie

doi:10.1128/spectrum.02039-21

Cited by 7 publications

(3 citation statements)

References 73 publications

(81 reference statements)

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“…Numerous studies have demonstrated that the microbes live in the genital tract are significantly associated with pregnancy outcomes of reproductive-aged women (5,(27)(28)(29), and they may also influence offspring's health in animal models (30)(31)(32).…”

Section: Discussionmentioning

confidence: 99%

The stability of lower genital tract (LGT) microbiota correlates with reproductive system function and in vitro fertilization and frozen embryo transfer outcomes in women with polycystic ovarian syndrome

Tu,

Zhang,

Chen

et al. 2024

Preprint

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We previously found that the lower genital tract (LGT) microbiota of polycystic ovarian syndrome (PCOS) women has altered when compared to healthy reproductive-aged women, however whether this alternation will have an impact on the reproductive system function and outcomes of PCOS patients have not yet been identified. Between 2018 and 2021, we totally enrolled 191 reproductive-aged women in three independent case-control studies. Firstly, we analyzed the correlation between LGT flora and clinical indexes of 97 women (containing 47 PCOS patients and 50 control reproductive-aged women), canonical correspondence analysis (CCA) showed that LH/FSH ratio was the most relevant factor that was related to the dominant genera in women with PCOS (r2=0.233, p=0.001), and menstrual cycle frequency was also strongly related to the LGT organisms (r2=0.116, p=0.005). Next, through analysis of 72 PCOS patients who were underwent IVF-FET therapy, the FET outcome in PNB group(PCOS with relative abundance of Lactobacillus ≥50%, n=57) was significant better than PDB group(PCOS patients with relative abundance of Lactobacillus<50%, n=15). Further, we randomly selected nine reproductive-aged PCOS patients (approximately half of them had altered LGT microbiota: Lactobacillus spp. ≤50%, n=5) and simultaneously collected both LGT swabs and cervical epithelial cells from them. By synchronization analysis of RNA sequencing of the cervical epithelial cells and 16S rRNA sequencing of the microbes, we found that the gene expression profiles of the cells significantly differed between the PDB (PCOS patients with relative abundance of Lactobacillus<50%, n=4) and PNB (PCOS with relative abundance of Lactobacillus ≥50%, n=5) groups.

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Section: Discussionmentioning

confidence: 99%

The stability of lower genital tract (LGT) microbiota correlates with reproductive system function and in vitro fertilization and frozen embryo transfer outcomes in women with polycystic ovarian syndrome

Tu,

Zhang,

Chen

et al. 2024

Preprint

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“…This bacterium has been associated with bacterial vaginosis [94][95][96][97] and with sPTB 54,98,99 . Indeed, in a recent study of the vaginal microbiota in midtrimester pregnant women in Nigeria, F. vaginae was the best predictor of preterm birth 54 , and in a study of rst trimester pregnant women in China, it was the best predictor of spontaneous abortion 100 . Aside from its ability to contribute to bio lm formation in women with bacterial vaginosis 101 , the virulence factors of F. vaginae remain largely unknown 102 .…”

Section: Discussionmentioning

confidence: 99%

The Vaginal Microbiota in Early Pregnancy Identifies a Subset of Women at Risk for Early Preterm Prelabor Rupture of Membranes and Preterm Birth

Romero¹,

Tarca

Gomez‐Lopez

et al. 2022

Preprint

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Spontaneous preterm birth (sPTB) can occur when vaginal bacteria gain access to the amniotic cavity. Thus, the predictive value of the vaginal microbiota for sPTB has been investigated, yet results have been inconclusive. Here, we report the largest study of the vaginal microbiota using longitudinal sampling of 257 cases and 514 controls (2,976 samples). Associations between the microbiota and sPTB were limited to cases of early (delivery <34 weeks) preterm prelabor rupture of membranes (PPROM). Starting with early gestation, microbial diversity was higher in early PPROM cases than controls, as were the relative abundances of Anaerococcus, Mobiluncus, Prevotella, and Sneathia. Random forest models based on data collected before 28 weeks predicted early PPROM [AUC=0.62(0.51−0.73)], while data collected by 24 weeks predicted PPROM with delivery <30 weeks [AUC=0.68(0.53-0.84)]. Therefore, monitoring of the vaginal microbiota profile may have clinical utility to identify a subset of women who will deliver a preterm neonate.

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“…One study found a high abundance of Atopobium in the vaginal microbiome samples of women with RPL as compared to controls [ 97 ], and another study reports that a relative abundance of >0.01% Atopobium could be used as a potential microbial biomarker to predict spontaneous miscarriages in the first trimester [ 102 ]. The potential immune-invasive role of Atopobium could be involved in disrupting the physicochemical barrier of the vaginal mucosa, thereby causing the release of pro-inflammatory cytokines and invasion of other anaerobes [ 102 ]. However, more research is needed on how the bacterial load of Atopobium affects pregnancy loss before it can be implemented as a diagnostic test.…”

Section: Repeated Implantation Failure and Recurrent Pregnancy Lossmentioning

confidence: 99%

Clinical Relevance of Vaginal and Endometrial Microbiome Investigation in Women with Repeated Implantation Failure and Recurrent Pregnancy Loss

Gao,

Louwers,

Laven

et al. 2024

IJMS

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Recent studies have investigated if and how the vaginal and endometrial microbiome might affect endometrial receptivity and reproductive health. Although there is no consensus on the existence of a core uterine microbiome yet, evidence shows that the dominance of Lactobacillus spp. in the female reproductive tract is generally associated with eubiosis and improved chances of successful implantation and an ongoing pregnancy. Conversely, vaginal and endometrial dysbiosis can cause local inflammation and an increase of pro-inflammatory cytokines, compromising the integrity and receptivity of the endometrial mucosa and potentially hampering successful embryonic implantation. This review provides a critical appraisal of the influence of the vaginal and endometrial microbiome as parts of the female reproductive tract on fertility outcomes, focusing on repeated implantation failure (RIF) and recurrent pregnancy loss (RPL). It seems that RIF as well as RPL are both associated with an increase in microbiome diversity and a loss of Lactobacillus dominance in the lower female reproductive system.

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Vaginal Atopobium is Associated with Spontaneous Abortion in the First Trimester: a Prospective Cohort Study in China

Abstract: Spontaneous abortion (SA) is the most common adverse pregnancy outcome in the first trimester. The causal drivers of SA have become a substantial challenge to reveal and overcome.

Cited by 7 publications

References 73 publications

The stability of lower genital tract (LGT) microbiota correlates with reproductive system function and in vitro fertilization and frozen embryo transfer outcomes in women with polycystic ovarian syndrome

The stability of lower genital tract (LGT) microbiota correlates with reproductive system function and in vitro fertilization and frozen embryo transfer outcomes in women with polycystic ovarian syndrome

The Vaginal Microbiota in Early Pregnancy Identifies a Subset of Women at Risk for Early Preterm Prelabor Rupture of Membranes and Preterm Birth

Clinical Relevance of Vaginal and Endometrial Microbiome Investigation in Women with Repeated Implantation Failure and Recurrent Pregnancy Loss

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