Vaginal Fibroblastic Cells from Women with Pelvic Organ Prolapse Produce Matrices with Increased Stiffness and Collagen Content

Ruiz‐Zapata, Alejandra M.; Kerkhof, Maxim P.A.M.; Ghazanfari, Samaneh; Zandieh-Doulabi, Behrouz; Stoop, Reinout; Smit, Theo H.; Helder, Marco N.

doi:10.1038/srep22971

Cited by 40 publications

(38 citation statements)

References 34 publications

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“…The images were taken using an inverted fluorescence microscope (Nikon TE 2000‐U, Nikon instruments Inc., USA). To evaluate the local fiber orientation, an Image J plug‐in called Orientation J was used …”

Section: Methodsmentioning

confidence: 99%

Evaluation of an elastic decellularized tendon‐derived scaffold for the vascular tissue engineering application

Ghazanfari

Alberti

et al. 2019

J Biomedical Materials Res

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Due to the limited success rate of currently available vascular replacements, tissue engineering has received tremendous attention in recent years. A main challenge in the field of regenerative medicine is creating a mechanically functional tissue with a well‐organized extracellular matrix, particularly of collagen and elastin. In this study, the native collagen scaffold derived from decellularized tendon sections, as a scaffold having the potential to be used for vascular tissue engineering applications, was studied. We showed that the elasticity of the scaffolds was improved when crosslinked with the bovine elastin. The effect of different concentrations of elastin on mechanical properties of the collagen scaffolds was evaluated of which 15% elastin concentration was selected for further analysis based on the results. Addition of 15% elastin to collagen scaffolds significantly decreased Young's modulus and the tensile stress at the maximum load and increased the tensile strain at the maximum load of the constructs as compared to those of the collagen scaffolds or control samples. Moreover, tubular elastin modified collagen scaffolds showed significantly higher burst pressure compared to the control samples. Smooth muscle cells and endothelial cells cultured on the elastin modified collagen scaffolds showed high viability (>80%) after 1, 3, and 7 days. Furthermore, the cells showed a high tendency to align with the collagen fibers within the scaffold and produced their own extracellular matrix over time. In conclusion, the results show that the decellularized tendon sections have a great potential to be used as scaffolds for vascular tissue engineering applications. © 2019 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 107A: 1225–1234, 2019.

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Section: Methodsmentioning

confidence: 99%

Evaluation of an elastic decellularized tendon‐derived scaffold for the vascular tissue engineering application

Ghazanfari

Alberti

et al. 2019

J Biomedical Materials Res

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“…The amount of ECM in the vaginal wall is responsible for supporting the pelvic floor and in advanced POP the remodeling capacity of vaginal fibroblasts is also adversely affected. 8 Oestrogen is the female sex hormone that plays a critical role in supporting the pelvic floor structures by controlling the rate of collagen remodeling. 9 Treatment with topical oestrogen in the form of creams prior to prolapse surgery has been shown to improve pelvic floor integrity compared to placebo.…”

Section: Introductionmentioning

confidence: 99%

Demonstration of improved tissue integration and angiogenesis with an elastic, estradiol releasing polyurethane material designed for use in pelvic floor repair

Shafaat

Mangır

Román

et al. 2018

Neurourology and Urodynamics

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“…In healthy women, the bladder is kept in place by the connective-tissue layer of the anterior vaginal wall which is a dense ECM with relatively few cells. The ECM obtains its strength from fibrillar proteins (collagen I and III), and is produced and maintained by fibroblastic cells (17,20). Abnormal collagen metabolism in SUI is well documented (21,22).…”

Section: Discussionmentioning

confidence: 99%

“…The vaginal wall consists of a dense ECM, rich in collagen, that is maintained and remodeled by fibroblastic cells. Fibroblasts are involved in normal and pathological soft tissue repair processes (17). Therefore, the present study was designed to determine AQP2 location and expression in the anterior vaginal wall, and investigate the association between AQP2 and the ECM in the pathogenesis of SUI.…”

Section: Introductionmentioning

confidence: 99%

Downregulation of AQP2 in the anterior vaginal wall is associated with the pathogenesis of female stress urinary incontinence

Zhang

Xie

et al. 2017

Molecular Medicine Reports

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The pathogenesis of stress urinary incontinence (SUI) is unclear. Aquaporins (AQPs) are a family of transmembrane proteins that transport water and small solutes, including glycerol, across cell membranes. AQPs have been demonstrated to serve a role in skin hydration, cellular proliferation, migration, immunity, wound healing and vascular remodeling in multiple organs. Furthermore, studies have confirmed that abnormal synthesis and degradation of collagens in extracellular matrix (ECM) remodeling contributes to SUI, by altering normal tissue architecture and mechanical properties. The authors previously demonstrated that AQP2 expressed in the human endometrium varies during the menstrual cycle. However, it is unknown whether AQP2 serves a role in the pathogenesis of SUI in the urethral supporting tissue. In the present study, AQP2 location and expression was examined in the anterior vaginal wall, and investigated the association between AQP2 and collagen I/III in female SUI. Western blotting, immunohistochemistry and immunofluorescence were used to measure AQP2 expression levels, and to reveal the location of AQP2 in the anterior vaginal wall, as well as fibroblasts in SUI and non‑SUI. The association between AQP2 and collagen I/III was subsequently investigated by AQP2‑small interfering RNA knockdown and overexpression 2 in fibroblasts. AQP2 expression in the anterior vaginal wall was significantly increased in women without SUI compared with those with SUI (P<0.05). Downregulation of AQP2 significantly decreased the mRNA and protein expression of collagen I/III, while AQP2 overexpression significantly increased the mRNA and protein expression of collagen I/III in fibroblasts (P<0.05). AQP2 was demonstrated to be expressed in the anterior vaginal wall and fibroblasts, and to regulate the expression level of collagen I/III in the anterior vaginal wall and fibroblasts, suggesting that AQP2 is associated with the pathogenesis of female SUI through collagen metabolism during ECM remodeling.

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Vaginal Fibroblastic Cells from Women with Pelvic Organ Prolapse Produce Matrices with Increased Stiffness and Collagen Content

Cited by 40 publications

References 34 publications

Evaluation of an elastic decellularized tendon‐derived scaffold for the vascular tissue engineering application

Evaluation of an elastic decellularized tendon‐derived scaffold for the vascular tissue engineering application

Demonstration of improved tissue integration and angiogenesis with an elastic, estradiol releasing polyurethane material designed for use in pelvic floor repair

Downregulation of AQP2 in the anterior vaginal wall is associated with the pathogenesis of female stress urinary incontinence

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