Vaginal cytology and cervical mucus as tools to predict ovulation time in small ruminants

Souza-Fabjan, J. M. G.; Brair, Viviane Lopes; Silva, Dafne Dos Santos; Schmidt, Ana Paula; Figueira, Lucas Machado; Rangel, Paulo Sérgio Cerqueira; Vergani, Gabriel Brun; Machado, Vitória de Oliveira; Oliveira, M. E. F.; Fonseca, J. F. da

doi:10.1007/s11250-021-02667-6

Cited by 5 publications

(2 citation statements)

References 7 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Cervical mucus was observed in most females (87.5%), con rming that they were in estrus. The mucus appearance is a physiological characteristic evaluated to determine the time of ovulation in small domestic ruminants, this being more accurate in goats 22 than in sheep 51 . Compared to data from domestic species, the mucus observed in the present study (crystalline and crystalline/striated) is related to initial moments of estrus, preceding ovulation by ~ 40 hours 22 , and may be an indicator of estrus synchrony among them.…”

Section: Discussionmentioning

confidence: 99%

Transcervical artificial insemination in the brown brocket deer (Subulo gouazoubira): A promising method for assisted reproduction in deer

duarte

Galindo

Baldini

et al. 2023

Preprint

Self Cite

View full text Add to dashboard Cite

The present study aimed to test the efficiency of transcervical artificial insemination techniques with cervical immobilization (TCAI-CI) or cervical traction (TCAI-CT), associated or not with the use of oxytocin (OT) as a protocol for cervical dilation, in the brown brocket deer (Subulo gouazoubira). The study was carried out in a crossover design using four adult females in two replicates with an interval of 60 days. Estrus was synchronized with oral melengestrol acetate (MGA) associated with estradiol benzoate and sodium cloprostenol. TCAI techniques were performed from 18 to 24 hours after estrus onset. All females received either an i.v. Apply 50 IU OT (G-OT, n = 4) or 1 mL saline solution (G-Control, n = 4) 20 minutes before the TCAI procedure. The TCAIs were performed using frozen-thawed semen from the same batch. Behavioral estrus was observed in 100% of females, within the two replicates, with a mean time of onset of 44.0 ± 22.0 h after cloprostenol application. The average length of the vagina was 13.0 ± 1.2 cm. It achieved a 50% (4/8) success of cervical transposition with semen deposition in the uterine. Regarding inseminations, most of them (87.5%) were performed using the TCAI-CT technique, and the overall conception rate was 50%. Cervical transposition times (< 1 minute) and TCAI procedures (~ 17 minutes) could be considered satisfactory. Thus, the TCAI-CI and TCAI-CT techniques performance was successful when applied in S. gouazoubira females, regardless of the use of OT as a cervical dilation protocol. This procedure is proposed as a method of choice for artificial insemination with greater applicability in different conservation centers, compared to more advanced reproductive biotechniques, and with a favorable impact on the conservation of deer species.

show abstract

Section: Discussionmentioning

confidence: 99%

Transcervical artificial insemination in the brown brocket deer (Subulo gouazoubira): A promising method for assisted reproduction in deer

duarte

Galindo

Baldini

et al. 2023

Preprint

Self Cite

View full text Add to dashboard Cite

show abstract

“…Moreover, intravaginal deposition reduces the hepatic metabolism and clearance of the hormones as they divert from the ‘first‐pass metabolism by the liver’ to the ‘first uterine pass effect’, wherein a high degree of direct transport from the vagina to uterine and ovarian vasculature occurs (Bulletti et al, 1997). During the oestrus phase, estrogens produced by the ovulatory follicle(s) promote growth and vascularization of the reproductive tract epithelium (Souza‐Fabjan et al, 2021), which further increases the rate of transvaginal absorption of drugs/hormones.…”

Section: Introductionmentioning

confidence: 99%

Luteal function in cyclic goats treated with human chorionic gonadotropin administered by intramuscular or intravaginal routes at the time of artificial insemination

Rodrigues

Guimarães

Rangel

et al. 2022

Reprod Domestic Animals

View full text Add to dashboard Cite

Human chorionic gonadotropin (hCG) has been used to improve goats reproductive efficiency. This study aimed to (i) evaluate if hCG administered by the intramuscular (i.m.) or intravaginal (i.vag.) route can be detected by a rapid β-hCG test in blood plasma samples and (ii) document ovarian effects of hCG administered by both routes at the time of artificial insemination (AI) performed 60 h after oestrus synchronization in goats. Twenty-two Alpine goats received two i.m. injections of 30 μg of dcloprostenol (Prolise®, Tecnopec, São Paulo, Brazil) 7.5 days apart. One day after the onset of oestrus (at the time of AI), the goats were randomly allocated to one of the three groups that received: control (n = 7): 0.3 ml of saline solution intravaginally; hCG i.m. (n = 7): 300 IU of hCG (Vetecor®; Hertape-Calier, São Paulo, Brazil) i.m. and hCG i.vag. (n = 8): 300 IU of hCG deposited intravaginally. Blood samples were drawn at −1, 3, 6, 9 and 24 h after as well as on days 3, 7, 10, 13, 17 and 21 after hCG treatment/ AI. All animals tested negative for hCG (ECO Diagnóstica, Corinto, Brazil) at −1 h, and all control animals tested negative throughout the entire blood collection period. All hCG i.m. animals tested positive from 3 h until D3 post-AI but only 50% of hCG i.vag.goats tested positive during the present study. In all animals studied, mean circulating P 4 concentrations increased (p < .05) from D3 to D7 after AI and then declined (p < .05) from D10 to D17 in control and hCG i.m. groups and from D17 to D21 in the hCG i.vag. group. Total cross-sectional luteal area (CLA), mean colour Doppler area (DA), DA/ CLA, mean high-velocity Doppler area and HVDA/CLA all declined (p < .05) by D17-D21 in all animals studied. In summary: (i) human chorionic gonadotropin could consistently be detected in blood samples using the rapid β-hCG test only in the hCG i.m. group; and (ii) there were no significant differences in the mean pregnancy rate, circulating P 4 concentrations and various luteal parameters studied among Control, hCG i.m. and hCG i.vag. dose.

show abstract

Characterization and analysis of transcriptomes of multiple tissues from estrus and diestrus in pigs

Liu,

Zhang,

Chen

et al. 2024

International Journal of Biological Macromolecules

View full text Add to dashboard Cite

Vaginal cytology and cervical mucus as tools to predict ovulation time in small ruminants

Cited by 5 publications

References 7 publications

Transcervical artificial insemination in the brown brocket deer (Subulo gouazoubira): A promising method for assisted reproduction in deer

Transcervical artificial insemination in the brown brocket deer (Subulo gouazoubira): A promising method for assisted reproduction in deer

Luteal function in cyclic goats treated with human chorionic gonadotropin administered by intramuscular or intravaginal routes at the time of artificial insemination

Characterization and analysis of transcriptomes of multiple tissues from estrus and diestrus in pigs

Contact Info

Product

Resources

About