Vaginal candidiasis in Konya area: Etiology, risk factors, virulence patterns, and antifungal susceptibility

Tokak, Semih; Kılıç, İbrahim; Horasanlı, Jule Eriç; Mutlu, Elif Gülbahçe; Taşbent, Fatma Esenkaya; Karagöz, Işık Didem

doi:10.2478/rrlm-2021-0012

Cited by 3 publications

(1 citation statement)

References 42 publications

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“…In the study carried out by, Tokak et al, caspofungin resistance was confirmed in 5.3% of C. albicans and 7.7% of C. glabrata isolates. 49 In this regard, more complementary and therapeutic studies should be done on the effectiveness of drugs alone and in combination to achieve the best results.…”

Section: Discussionmentioning

confidence: 99%

Vulvovaginal candidiasis in Iranian women: Molecular identification and antifungal susceptibility pattern

Baniasadi

Mosavi

Sharifi

et al. 2022

J of Obstet and Gynaecol

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Aim Vulvovaginal candidiasis (VVC), is a common fungal infection that remains a global concern. The objectives of this study were molecular identification and assessment of the antifungal susceptibility profile of Candida species, causing VVC in southeast Iran. Methods A cross‐sectional investigation was carried out on 119 nonpregnant females suspected of VVC between February 2019 and May 2021. Yeast samples were characterized to the species level by conventional and molecular methods. All Candida isolates were examined for in vitro susceptibility profile to six conventional antifungal drugs using Clinical and Laboratory Standards Institute guidelines. Results Out of 119 subjects, 52 (43.7%) cases were affected by VVC, out of whom 11 (21.15%) cases had recurrent vulvovaginal candidiasis (RVVC). The species distribution was as follows; Candida albicans (n = 21; 40.4%), C. glabrata (n = 11; 21.2%), C. tropicalis (n = 9; 17.3%), C. parapsilosis (n = 5; 9.7%), C. africana (n = 3; 5.7%), C. famata (n = 1; 1.9%), C. lusitaniae (n = 1; 1.9%), and C. dubliniensis (n = 1; 1.9%). The resistance rate of Candida isolates to fluconazole, itraconazole, and voriconazole were 15.38%, 11.5%, and 3.8%, respectively. Resistance to fluconazole was obtained in 46% (5/11) of RVVC cases but only in 7% (3/41) of VVC cases. Conclusion This study demonstrated that the majority of VVC cases were caused by non‐albicans Candida species which also were resistant to some antifungal agents. Hence, our findings revealed the importance of conducting periodical epidemiological studies to determine changes in species distribution. Moreover, for effective management of treatment and infection, it is imperative to evaluate the susceptibility profiles of Candida species isolated from VVC patients.

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Section: Discussionmentioning

confidence: 99%

Vulvovaginal candidiasis in Iranian women: Molecular identification and antifungal susceptibility pattern

Baniasadi

Mosavi

Sharifi

et al. 2022

J of Obstet and Gynaecol

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Molecular Detection of INT, HWP, PLB, SAP and ALS Genes in Candida Species Isolated from Patients with Vulvovaginal Candidiasis

TOKAK,

KILIÇ,

GÜLBAHÇE MUTLU

et al. 2023

Kırıkkale Üniversitesi Tıp Fakültesi Dergisi

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Amaç: Vulvovajinal kandidiyazis, kadınların yaklaşık %75’inde yaşamları boyunca en az bir kez görülen yüzeysel mantar enfeksiyonudur. Bazı genler, Candida türlerinin vajina mukozasına ve epitel hücrelerine yapışmasında ve patogenezinde rol oynamaktadır. Bu çalışmanın amacı, vulvovajinal kandidiyazisli hastalardan izole edilen Candida suşlarındaki HWP1 (hifal duvar proteini), ALS1 (agglutinin benzeri sekans), SAP1 (sekrete aspartil proteaz), PLB1 (fosfolipaz B), INT1 (integrin A) genlerinin sıklığının araştırılmasıdır. Gereç ve Yöntemler: Çalışmaya moleküler teknik kullanılarak tanımlanan 100 vajinal Candida izolatı dahil edildi. İzole edilen tüm Candida suşlarında, enfeksiyon da önemli rol oynayan INT1, HWP1, PLB1, SAP1 ve ALS1 genlerinin varlığı PCR yöntemi kullanılarak araştırıldı. Bulgular: SAP1, INT1 ve HWP1 virülans genleri Candida suşları arasında en yaygın, PLB geni en az tespit edilen gen olmuştur. Candida albicans suşlarında SAP1 (%93.0) geni en çok saptanan virülans geni olurken, bunu HWP1 geni (%86) takip etmiştir. Benzer şekilde non-albicans Candida türlerinde saptanan virülans genleri arasında SAP1 geni (%86) en fazla saptanan virülans geni olmuştur. Farklı Candida türlerinin %95’inde VVK’ya neden olabilecek en az iki virülans faktörü tanımlanmıştır. Sonuç: Bu çalışmada elde edilen sonuçlar, vulvovajinal kandidiyazis enfeksiyonu sırasında eksprese edilen INT, HWP, PLB, SAP ve ALS genlerinin, Candida suşlarının vajinaya adezyonuna ve biyofilm oluşumuna katkıda bulunmuş olabileceğini düşündürmektedir.

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Actividad de fosfolipasas y proteasas en aislamientos de especies de Candida colonizadoras y causantes de vulvovaginitis en mujeres gestantes

Puello,

Young,

Suárez

2022

biomedica

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Introducción. Las proteasas y las fosfolipasas son factores de virulencia de Candida spp. que cumplen un papel importante en la invasión de los tejidos. Entre los factores relacionados con el huésped, se encuentran algunos asociados con las características ambientales y otros con la colonización.Objetivo. Determinar la actividad de fosfolipasas y proteasas en aislamientos de especies colonizadoras y patógenas de Candida spp., aisladas de mujeres gestantes de Cartagena de Indias.Materiales y métodos. Se determinó la actividad de fosfolipasas y proteasas en 56 aislamientos mediante degradación del sustrato y cálculo del coeficiente de actividad enzimática. Se compararon las actividades de fosfolipasas y proteasas, entre los aislamientos colonizadores y los patógenos.Resultados. La actividad de la fosfolipasa fue “muy alta” (< 0,69) en 34 aislamientos e, igualmente, la de la proteasa en 14. No hubo diferencias significativas al comparar las actividades de las fosfolipasas y de las de las proteasas, entre los aislamientos colonizadores y los patógenos.Conclusiones. La actividad de las fosfolipasas predominó como factor de virulencia en los aislamientos estudiados. No obstante, no se encontró una diferencia significativa entre los grupos de aislamientos colonizadores y los patógenos, en cuanto a las actividades de fosfolipasas y proteasas.

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Vaginal candidiasis in Konya area: Etiology, risk factors, virulence patterns, and antifungal susceptibility

Cited by 3 publications

References 42 publications

Vulvovaginal candidiasis in Iranian women: Molecular identification and antifungal susceptibility pattern

Vulvovaginal candidiasis in Iranian women: Molecular identification and antifungal susceptibility pattern

Molecular Detection of INT, HWP, PLB, SAP and ALS Genes in Candida Species Isolated from Patients with Vulvovaginal Candidiasis

Actividad de fosfolipasas y proteasas en aislamientos de especies de Candida colonizadoras y causantes de vulvovaginitis en mujeres gestantes

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