Vaginal bacterial flora and cytology in proestrous bitches: Role on fertility

Groppetti, D.; Pecile, A.; Barbero, César Alberto; Martino, Piera Anna

doi:10.1016/j.theriogenology.2011.11.022

Cited by 34 publications

(68 citation statements)

References 13 publications

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“…Vaginal microbiota findings in our work were similar to the findings reported by other authors (Carneiro, Toniollo, & Schocken‐Iturrino, ; Groppetti et al., ; Laurusevičius, Jūratė, & Henrikas, ; Watts, Wright, & Whithear, ). Staphylococcus spp.…”

Section: Discussionsupporting

confidence: 92%

“…The percentage of beta‐haemolytic Streptococcus observed in the present study in S1 in G1 and G2 were higher from those reported by Groppetti et al. (), who founded a smaller percentage (20.6%) in healthy bitches during proestrus.…”

Section: Discussioncontrasting

confidence: 91%

“…On the other hand, E. coli was found by Groppetti et al. () during proestrus in lower percentages than in the current study (2.9%). In the present study, beta‐haemolytic Streptococcus was the bacteria more frequently isolated.…”

Section: Discussioncontrasting

confidence: 80%

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Vaginal isolation of beta‐haemolytic Streptococcus from bitches with and without neonatal deaths in the litters

Guerrero¹,

Stornelli²,

Jurado³

et al. 2018

Reprod Domestic Animals

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The aim of the study was to identify beta-haemolytic streptococci in the vagina of bitches who had delivered healthy litters and bitches who had delivered litters in which neonatal deaths occurred. Fifty-one bitches divided into two groups were used. Group 1 (G1) included 28 bitches that had delivered healthy litters and group 2 (G2) included 23 bitches that had delivered puppies who died in the neonatal period. Two vaginal samples were taken, one in proestrus and the other at the end of gestation (EG). Beta-haemolytic Streptococcus (BS) was isolated from 16 bitches (57%) in G1 and from 21 bitches (91%) in G2. The bacteriological cultures, serological tests (Streptex ) and PCR assay allowed identification of Streptococcus canis and Streptococcus dysgalactiae in G1 and G2. Ultramicroscopic studies allowed the observation of M Protein and capsules in strains of S. dysgalactiae and S. canis in G1 and G2. The S. canis strains isolated from G2 showed thicker capsules than S. canis strains isolated from G1 (234 ± 24.2 vs 151.23 ± 28.93 nm; p < .001.). No differences were observed in capsule thickness between strains of S. dysgalactiae isolated from G1 and G2 (210 ± 13.54 vs 211.66 ± 19.67 nm; p > .70). All strains of beta-haemolytic Streptococcus isolated were penicillin sensitive. Penicillin was administered from EG to 5 days post-partum in 10 G2 females with isolation of BS (G2A). Saline solution was administered in eleven G2 females with isolation of BS (G2B). Ninety per cent of the puppies survived in G2A and 25% survived in G2B. Our results suggest BS is involved in canine neonatal deaths.

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Section: Discussionsupporting

confidence: 92%

Section: Discussioncontrasting

confidence: 91%

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Vaginal isolation of beta‐haemolytic Streptococcus from bitches with and without neonatal deaths in the litters

Guerrero¹,

Stornelli²,

Jurado³

et al. 2018

Reprod Domestic Animals

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“…), Moraxella spp., Flavobacterium spp., Pseudomonas spp., Proteus spp., Corynebacterium spp., Enterobacter spp., Bacillus spp., Klebsiella spp., Neisseria spp., Pasteurella spp., Ureaplasmataceae , Bacteroides spp., Peptostreptococcus spp., lactobacilli and Mycoplasma spp. (Bruchim, Lutsky, & Rosendal, ; Baba, Hata, Fukata, & Arakawa, ; van Duijkeren, ; Root Kustritz, ; Groppetti, Pecile, Barbero, & Martino, ). Although it is generally accepted that the vaginal microbiota almost represents the bacterial micro‐organisms residing in the uterine in prooestrus and oestrus, data about the uterine microbiota are controversial with some authors reporting bacterial findings only rarely (Olson & Mather, ) and others frequently (Watts, Wright, & Whithear, ; Watts, Wright, Lee, & Whithear, ; Watts, Wright, & Lee, ; Schultheiss, Jones, Kesel, & Olson, ).…”

Section: Introductionmentioning

confidence: 99%

Bacteriological findings in the canine uterus during Caesarean section performed due to dystocia and their correlation to puppy mortality at the time of parturition

Goericke-Pesch

Fux

Prenger‐Berninghoff

et al. 2018

Reprod Domestic Animals

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Canine intrauterine bacteriological flora during dystocia is unknown. Thus, frequency (bacterial growth (not) detected), quality (species and number of different bacterial isolates) and quantity (colony-forming units) of intrauterine bacteria in relation to in utero foetal death in 50 bitches undergoing emergency Caesarean section were investigated. Bacterial growth was quantified from single colonies, (+) (0.5), to strong growth, +++ (3) and was observed in 34 bitches (68%), with Staph. epidermidis (n = 12), Staph. intermedius-group (n = 7), β-haemolytic streptococci (n = 6), Staph. aureus, α- and γ-haemolytic streptococci (n = 4 each) being most common and one to four bacteria per sample. Regarding the quantity, most often (n = 46) low growth was identified. In bitches with living pups only (group I), mean number of isolates was 0.78 ± 0.83 compared to 1.60 ± 1.10 (living + stillborn pups, group II) and 1.0 ± 1.15 (stillborn pups only, group III) and mean bacterial growth in groups I/II/III was + (1.0, quantity), + (1.4) and ++ (1.6). Taking just positive samples into consideration, mean number of bacterial isolates was significantly higher in group II compared to I (p = .0088). We concluded that the canine uterus cannot be considered free of bacteria during dystocia. Mean numbers of different bacterial isolates and quantity of bacterial growth are higher in bitches with in utero foetal death.

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“…Exfoliative vaginal cytology is the most popular diagnostic method as a part of the gynaecological examination in the bitch (Dreier, 1975;Gunzel-Apel & Koivisto, 1984;Linde & Karlson, 1984;Soderberg, 1986a,b;Tammer et al, 1994;Johnson, 2006;Wehrend, 2007;Trasch, 2008;Turmalaj et al, 2011;Groppetti et al, 2012;Leigh et al, 2013;Wehrend et al, 2013). It is based on determination of cyclic cellular changes occurring in the vaginal epithelium as a result of reproductive hormone levels, especially estrogens (Wright & Parry, 1989).…”

Section: Introductionmentioning

confidence: 99%

Application of exfoliative vaginal cytology in clinical canine reproduction – a review

Antonov¹

2017

BJVM

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SummaryAntonov, A. L., 2017. Application of exfoliative vaginal cytology in clinical canine reproduction -a review. Bulg. J. Vet. Med., 20, No 3,[193][194][195][196][197][198][199][200][201][202][203] Vaginal cytology has many practical applications in the evaluation of both the normal and abnormal bitch. The objective of this review is to describe the use of exfoliative vaginal cytology as a diagnostic tool in clinical canine reproduction.

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Vaginal bacterial flora and cytology in proestrous bitches: Role on fertility

Cited by 34 publications

References 13 publications

Vaginal isolation of beta‐haemolytic Streptococcus from bitches with and without neonatal deaths in the litters

Vaginal isolation of beta‐haemolytic Streptococcus from bitches with and without neonatal deaths in the litters

Bacteriological findings in the canine uterus during Caesarean section performed due to dystocia and their correlation to puppy mortality at the time of parturition

Application of exfoliative vaginal cytology in clinical canine reproduction – a review

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