2018
DOI: 10.21767/2573-0282.100064
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vAetiology of Diarrhoea in Children Under Five in Zambia Detected Using Luminex xTAG Gastrointestinal Pathogen Panel

Abstract: Background: We aimed to document viral, bacterial, and protozoan enteric pathogens responsible for causing moderate-to-severe diarrhoea among children under five presenting at public health facilities in Zambia following the introduction of rotavirus vaccination.

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Cited by 24 publications
(33 citation statements)
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“…However, it must be emphasized that our study was entirely conducted in a very disadvantaged community with poor sanitation and very few resources. We are aware of only one other study in Zambia using the same diagnostic tool 33 ; that study included rural areas and a broader range of urban socio-economic conditions and found carriage rates about half of those reported in this study. Molecular diagnosis has been widely used for analysis of enteropathogen burden and the xTAG GPP panel has been reported to deliver accurate pathogen detection 34 , 35 .…”
Section: Discussionmentioning
confidence: 99%
“…However, it must be emphasized that our study was entirely conducted in a very disadvantaged community with poor sanitation and very few resources. We are aware of only one other study in Zambia using the same diagnostic tool 33 ; that study included rural areas and a broader range of urban socio-economic conditions and found carriage rates about half of those reported in this study. Molecular diagnosis has been widely used for analysis of enteropathogen burden and the xTAG GPP panel has been reported to deliver accurate pathogen detection 34 , 35 .…”
Section: Discussionmentioning
confidence: 99%
“…Samples were shipped frozen with temperatures monitors to the Georgia Institute of Technology (Atlanta, USA) where we used the xTAG GPP (Luminex Corp, Austin, USA), a qualitative multiplex molecular assay, to detect 15 enteric pathogens in stool samples: Campylobacter jejuni/coli/lari; Clostridium difficile , toxin A/B; enterotoxigenic Escherichia coli (ETEC) LT/ST; Shiga-like toxin producing E. coli (STEC) stx1/stx2; E. coli O157; Salmonella; Shigella boydii/sonnei/flexneri/dysenteriae; Vibrio cholerae; Yersinia enterocolitica; Giardia lamblia; Cryptosporidium parvum/hominis; Entamoeba histolytica; adenovirus 40/41; norovirus GI/GII; and rotavirus. The GPP has been rigorously tested and extensively used for stool-based enteric pathogen detection ( Chisenga et al, 2018 ; Claas, 2013 ; Deng et al, 2015 ; Duong et al, 2016 ; Huang et al, 2016 ; Kellner et al, 2019 ; Khare et al, 2014 ; Navidad et al, 2013 ; Patel et al, 2014 ). We analyzed samples according to manufacturer instructions with the addition of elution steps for the pretreatment of rectal swabs and diaper material saturated with liquid stool (Appendix 1- Consent procedures, survey administration, and specimen collection and analysis).…”
Section: Methodsmentioning
confidence: 99%
“…Acute diarrhea can be caused by different types of pathogens, among them the major five enteric pathogens Rotavirus (67.6%), Adenovirus (41.5%), enterotoxigenic E. coli (40.7%), Salmonella (38.4%), and Giardia (37.0%) [ 1 ]. Globally, acute diarrhea is the leading cause of morbidity and mortality among under-five children.…”
Section: Introductionmentioning
confidence: 99%