Vacuoles and Storage Organelles

Noguchi, Tetsuko; Hayashi, Yasuko

doi:10.1007/978-4-431-54941-3_5

Cited by 3 publications

(2 citation statements)

References 17 publications

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“…They are surrounded by vacuolar membranes and filled with dissolved substances, including hydrolytic enzymes. Once released from the vacuoles, these enzymes hydrolyze certain cellular components [ 28 , 29 ]. Low night temperature (9°C) caused increased numbers of vacuoles in intermediary cells, which later fused to a central vacuole.…”

Section: Discussionmentioning

confidence: 99%

Low Night Temperature Affects the Phloem Ultrastructure of Lateral Branches and Raffinose Family Oligosaccharide (RFO) Accumulation in RFO-Transporting Plant Melon (Cucumismelo L.) during Fruit Expansion

Hao

Zhu

et al. 2016

PLoS ONE

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Due to the importance and complexity of photo assimilate transport in raffinose family oligosaccharide (RFO)-transporting plants such as melon, it is important to study the features of the transport structure (phloem) particularly of the lateral branches connecting the source leaves and the sink fruits, and its responses to environmental challenges. Currently, it is unclear to what extents the cold environmental temperature stress would alter the phloem ultrastructure and RFO accumulation in RFO-transporting plants. In this study, we firstly utilized electron microscopy to investigate the changes in the phloem ultrastructure of lateral branches and RFO accumulation in melons after being subjected to low night temperatures (12°C and 9°C). The results demonstrated that exposure to 9°C and 12°C altered the ultrastructure of the phloem, with the effect of 9°C being more obvious. The most obvious change was the appearance of plasma membrane invaginations in 99% companion cells and intermediary cells. In addition, phloem parenchyma cells contained chloroplasts with increased amounts of starch grains, sparse cytoplasm and reduced numbers of mitochondria. In the intermediary cells, the volume of cytoplasm was reduced by 50%, and the central vacuole was present. Moreover, the treatment at 9°C during the night led to RFO accumulation in the vascular bundles of the lateral branches and fruit carpopodiums. These ultrastructural changes of the transport structure (phloem) following the treatment at 9°C represented adaptive responses of melons to low temperature stresses. Future studies are required to examine whether these responses may affect phloem transport.

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Section: Discussionmentioning

confidence: 99%

Low Night Temperature Affects the Phloem Ultrastructure of Lateral Branches and Raffinose Family Oligosaccharide (RFO) Accumulation in RFO-Transporting Plant Melon (Cucumismelo L.) during Fruit Expansion

Hao

Zhu

et al. 2016

PLoS ONE

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“…Vacuoles are membrane‐bound organelles that contain inorganic ions and organic compounds. Their morphology and biochemistry, as well as the materials they store, vary greatly depending on the cell type and stage (Noguchi & Hayashi, 2014). The vacuolar membrane, also called tonoplast, contains transport proteins with different functions, which are often considered key players in mechanisms of molecules storage in and release from the vacuolar environment (Behrenfeld et al ., 2021).…”

Section: Introductionmentioning

confidence: 99%

The tonoplast localized protein PtNPF1 participates in the regulation of nitrogen response in diatoms

Santin,

Russo,

de los Ríos

et al. 2023

New Phytologist

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Summary Diatoms are a highly successful group of phytoplankton, well adapted also to oligotrophic environments and capable of handling nutrient fluctuations in the ocean, particularly nitrate. The presence of a large vacuole is an important trait contributing to their adaptive features. It confers diatoms the ability to accumulate and store nutrients, such as nitrate, when they are abundant outside and then to reallocate them into the cytosol to meet deficiencies, in a process called luxury uptake. The molecular mechanisms that regulate these nitrate fluxes are still not known in diatoms. In this work, we provide new insights into the function of Phaeodactylum tricornutum NPF1, a putative low‐affinity nitrate transporter. To accomplish this, we generated overexpressing strains and CRISPR/Cas9 loss‐of‐function mutants. Microscopy observations confirmed predictions that PtNPF1 is localized on the vacuole membrane. Furthermore, functional characterizations performed on knock‐out mutants revealed a transient growth delay phenotype linked to altered nitrate uptake. Together, these results allowed us to hypothesize that PtNPF1 is presumably involved in modulating intracellular nitrogen fluxes, managing intracellular nutrient availability. This ability might allow diatoms to fine‐tune the assimilation, storage and reallocation of nitrate, conferring them a strong advantage in oligotrophic environments.

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Environmental impacts on carotenoid metabolism in leaves

Dhami

Cazzonelli

2020

Plant Growth Regul

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Vacuoles and Storage Organelles

Cited by 3 publications

References 17 publications

Low Night Temperature Affects the Phloem Ultrastructure of Lateral Branches and Raffinose Family Oligosaccharide (RFO) Accumulation in RFO-Transporting Plant Melon (Cucumismelo L.) during Fruit Expansion

Low Night Temperature Affects the Phloem Ultrastructure of Lateral Branches and Raffinose Family Oligosaccharide (RFO) Accumulation in RFO-Transporting Plant Melon (Cucumismelo L.) during Fruit Expansion

The tonoplast localized protein PtNPF1 participates in the regulation of nitrogen response in diatoms

Environmental impacts on carotenoid metabolism in leaves

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