Vacuolation and the release of heparin in mast cells cultivated in vitro

Paff, George H.; Bloom, Frank

doi:10.1002/ar.1091040106

Cited by 38 publications

(7 citation statements)

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“…There are also other differences in the shape and structure of the nucleus and staining reaction of these two cells.12* Like Doan and Reinhardt, most workers in this field have been careful to note that there are marked differences between the two types of basophil staining cells.112g 142 In recent years, however, many of the so-called differences between the two types of cells are beginning to be resolved. For example, tissue culture studies of the mast cells by Paff and Bloom (1949), 169 and Zitcer (1953), 244 have indicated that these cells are quite active and vary tremendously in size and shape. The granulation of the cells varies in a somewhat cyclic manner.…”

Section: Basopkil Cellsmentioning

confidence: 99%

Physiological Approaches to an Understanding of the Function of Eosinophils and Basophils

Speirs

1955

Annals of the New York Academy of Sciences

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Section: Basopkil Cellsmentioning

confidence: 99%

Physiological Approaches to an Understanding of the Function of Eosinophils and Basophils

Speirs

1955

Annals of the New York Academy of Sciences

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“…mals( 5- 7) , and under a variety of conditions (1,2,(8)(9)(10)(11). In none of the present studies were there statistically significant changes in total mast cell number.…”

Section: Resultsmentioning

confidence: 99%

Influence of Hypophysis and of Adrenal Cortex upon Tissue Mast Cell of the Hamster.

Smith

Lewis

1955

Experimental Biology and Medicine

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The finding ( 1-3 ) of changes in the number and cytology of tissue mast cells after administration of ACTH and cortisone has suggested that the pituitary-adrenal system exerts a controlling influence on the mast cell. In exploring this possibility further we have found, in the rat, that hypophysectomy or administration of ACTH or cortisone elicited small increases in the number of abnormal mast cells (as evidenced by vacuolation and clumping of cytoplasmic granules) (4). The bulk of the evidence indicated, however, that the ACTH effect was not mediated by the adrenal cortex (4). The present report is concerned with similar experiments in the Syrian hamster. We have found marked changes in the number of abnormal mast cells in the mesentery, skin, and cheek pouch as a result of ACTH or cortisone treatment. There is strong evidence that the ACTH effect is mediated through the adrenal cortex.Methods. Examinations of the behavior of the tissue mast cells were made in whole mounts(5) of skin, mesentery, and cheek pouch of young, adult, male Syrian hamsters (100 g) at various times after adrenalectomy, hypophysectomy, or treatment with ACTH or cortisone. Adrenalectomy was carried out in 2 stages at least one week apart. Adrenalec-*Performed under auspices of the U. S. Atomic Energy Commission. t With the assistan,ce of Sally T. Hartig.tomized hamsters given either tap water or 1% NaCl solution to drink died within one week. Subcutaneous implantation of 15-mg pellets of desoxycorticosterone acetate (DOCA) at time of removal of the second adrenal, however, was followed by weight gain and prolonged survival. (All survived for 34 days, the duration of the experiment.) These animals had only tap water to drink. Hypophysectomized animals and their controls were given 20% sucrose solution to drink. In addition to the regular diet of Rockland checkers, given to adrenalectomized and control animals, hypophysectomized animals had access to orange slices, lettuce, bread, and ground meat. Tissues were fixed in alcohol and stained with toluidine blue as previously described( 5 ) . Tissues from intact or mockoperated controls were simultaneously studied. Mast cell counts were done on the stained tissues, note being made of normal cells and atypical cells (degenerated cells with vacuoles and clumped granules) (5,6). On a single slide from each animal, 30 contiguous fields, 0.0676 mm2 each, were counted at a magnification factor of 264.The only change in mast cells which could be identified with certainty was an increase in the number of cells showing vacuolation and conglomeration of cytoplasmic granules. These alterations were similar to those previously found in irradiated ani-Results.

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“…mast cells ( 6) , edematous tissue and stimulated urticaria pigmentosa lesions ( 7), and after treatment with toluidine blue( 8), nitrogen mustard (9), ACTH ( 1 ) and cortisone ( 1,2 ) .…”

Section: Resultsmentioning

confidence: 99%

Influence of Hypophysis and Adrenal Cortex upon Tissue Mast Cell of the Rat

Smith¹,

Lewis²

1954

Experimental Biology and Medicine

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Finding( 1-3) of changes in number and cytology of tissue mast cells after administration of adrenworticotropic hormone ( lAICTH) and cortisone has suggested that the pituitaryadrenal system exerts a controlling influence on the mast cell. The present paper describes attempts to explore this possibility further.Methods. Examinations of the behavior of tissue mast cell were made in whole mounts (4) of skin and mesentery of young adult, male Sprague-Dawley rats (20'0 g) at various times after adrenlalectomy, hypophysectomy and treatment with X-irradiation, ACTH, adrenal cortical extract, desoxycorticosterone acetate or cortisone. Adrenalectomized rats and their controls were allowed to drink only 1 a/o NaCl solution; hypophysectomized anim l s and their controls were confined to 20% sucrose solution. In addistion to regular diet of Rockland checkers, the hypophysectomid animals had access to oranges, lettuce, bread, and ground meat. Tissues were fixed in alcohol and stained with toluidine blue, as previously described (4). Tissues from intact or mock-operated controls were simultaneously studied. The radiation factors were 250 kv, 15 ma, 0.5 mm Cu and 3.0 mm Bakelite filters, 26.7 cm target distance, 1.5 mm Cu half-value layer and 215-225 r per minute. Mast cell counts were made on stained tissues, separate note taken of normal cells and atypical cells, i.e. degenerated cells with vacuoles and clumped granules(4,S). On a single slide from each animal, thirty contiguous fields, 0.0676 mm2 each, were counted at a magnification factor of 264.The only change in mast cells which could be identified with certainty was an increase in number of cells showing vacuolation and conglomeration of cytoplasmic granules. The allteratims were similar to those previously found by us in irradiated animals (4) and by others in aged tissue cultures of Results.*With assistance of Sally T. Hartig.mast cells ( 6) , edematous tissue and stimulated urticaria pigmentosa lesions ( 7), and after treatment with toluidine blue( 8), nitrogen mustard (9), ACTH ( 1 ) and cortisone ( 1,2 ) .In none of the present studies were there statistically significant changes in total mast cell number.Adrenalectomy was without influence upun the mast cell or upon its response to X-irradiation. Groups of 3 to 101 rats were examined at 2, 7, 14, 21, 28 and 316 days after a d r e n a b 'tomy. Number of cells countedr2.028 mm2 of skin varied between 404 land 6 10 in adrenalectomized and 458 and 608 in mock-operated animals. The per cent of abnormal cells was approximately the same in both groups of animals (.2 to 1.2% of all cells). At 2 weeks after adrenalectomy or mock-operation rats were subjected to single, total-body exposures to 600 r. Groups of 3 rats were examined at various intervals after irradiation. Number of cells counted/2.028 mm2 of skin varied between 328 and 676 in adrenalectomized, irradiated land 339 and 8316 in mock-operated, irradiated rats. The per cent of abnormal cells was 10, 33.3,33.6, 7.0, 3.4 and 2.9 in the adrenalectomized and 10.1, 42...

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Vacuolation and the release of heparin in mast cells cultivated in vitro

Cited by 38 publications

References 2 publications

Physiological Approaches to an Understanding of the Function of Eosinophils and Basophils

Physiological Approaches to an Understanding of the Function of Eosinophils and Basophils

Influence of Hypophysis and of Adrenal Cortex upon Tissue Mast Cell of the Hamster.

Influence of Hypophysis and Adrenal Cortex upon Tissue Mast Cell of the Rat

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