Vacuolar-Type H+-ATPase and Na+, K+-ATPase Expression in Gills of Atlantic Salmon (Salmo salar) during Isolated and Combined Exposure to Hyperoxia and Hypercapnia in Fresh Water

Seidelin, Michel; Brauner, Colin J.; Frank, Jason; Madsen, Steffen S.

doi:10.2108/zsj.18.1199

Cited by 24 publications

(14 citation statements)

References 30 publications

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“…For example, Na ϩ -K ϩ -ATPase-␣ 1a and NKCC1 mRNA expression increase only transiently in the gills of killifish after SW transfer, whereas expression of these genes increases for more prolonged periods in the gills of Atlantic salmon (6,51,58), brown trout (Salmo trutta) (28,58), or European sea bass (Dicentrarchus labrax) (19). Transient changes in killifish gill Na ϩ -K ϩ -ATPase activity after SW transfer were also observed in this study and in others (29,59), whereas more prolonged increases in Na ϩ -K ϩ -ATPase activity are typical of many fish species (19,24,28,47,50,51). The transient nature of many changes observed in gill protein expression and activity appears to be an important response of killifish to SW transfer and may reflect greater tolerance to salinity change.…”

Section: Discussionsupporting

confidence: 76%

Changes in gene expression in gills of the euryhaline killifishFundulus heteroclitusafter abrupt salinity transfer

Scott¹,

Richards²,

Forbush³

et al. 2004

American Journal of Physiology-Cell Physiology

206

168

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Maintenance of ion balance requires that ionoregulatory epithelia modulate ion flux in response to internal or environmental osmotic challenges. We have explored the basis of this functional plasticity in the gills of the euryhaline killifish Fundulus heteroclitus. The expression patterns of several genes encoding ion transport proteins were quantified after transfer from near-isosmotic brackish water [10 parts/thousand (ppt)] to either freshwater (FW) or seawater (SW). Many changes in response to SW transfer were transient. Increased mRNA expression occurred 1 day after transfer for Na(+)-K(+)-ATPase-alpha(1a) (3-fold), Na(+)-K(+)-2Cl(-)-cotransporter 1 (NKCC1) (3-fold), and glucocorticoid receptor (1.3-fold) and was paralleled by elevated Na(+)-K(+)-ATPase activity (2-fold). The transient increase in NKCC1 mRNA expression was followed by a later 2-fold rise in NKCC protein abundance. In contrast to the other genes studied in the present work, mRNA expression of the cystic fibrosis transmembrane conductance regulator (CFTR) Cl(-) channel generally remained elevated (2-fold) in SW. No change in protein abundance was detected, however, suggesting posttranscriptional regulation. The responses to FW transfer were quite different from those to SW transfer. In particular, FW transfer increased Na(+)-K(+)-ATPase-alpha(1a) mRNA expression and Na(+)-K(+)-ATPase activity to a greater extent than did SW transfer but had no effect on V-type H(+)-ATPase expression, supporting the current suggestion that killifish gills transport Na(+) via Na(+)/H(+) exchange. These findings demonstrate unique patterns of ion transporter expression in killifish gills after salinity transfer and illustrate important mechanisms of functional plasticity in ion-transporting epithelia.

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Section: Discussionsupporting

confidence: 76%

Changes in gene expression in gills of the euryhaline killifishFundulus heteroclitusafter abrupt salinity transfer

Scott¹,

Richards²,

Forbush³

et al. 2004

American Journal of Physiology-Cell Physiology

206

168

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“…A similar shift to more ATPefficient ion transporters was suggested in OA-exposed oysters due to the observed lowering of extracellular [Na þ ] and elevation of [K þ ] compared to controls (Lannig et al, 2010). In fish, elevated Na þ/ K þ ATPase activity was found in some species after acclimation to long-term hypercapnia (1e5 kPa P CO2 ) (Ishimatsu et al, 2005;Deigweiher et al, 2008), while in others Na þ/ K þ ATPase did not change (2 kPa P CO2 ) (Seidelin et al, 2001). In cod, Na þ /K þ ATPase activity in gill cells was not affected at 0.3 kPa P CO2 , but increased at 0.6 kPa P CO2 (Melzner et al, 2009).…”

Section: Ocean Acidificationsupporting

confidence: 63%

Energy homeostasis as an integrative tool for assessing limits of environmental stress tolerance in aquatic invertebrates

Sokolova

Frederich

Bagwe

et al. 2012

Marine Environmental Research

1,003

718

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“…The data for Na + K + -ATPase regulation under hypercapnia are sparse and contradictory suggesting species-specific thresholds in CO 2 -induced impact on Na + K + -ATPase capacity. Thus, in some species elevated CO 2 levels have no effect on activity of Na + K + -ATPase (2 kPa P co 2 , [94]) whereas in others increased Na + K + -ATPase activity was observed after acclimation to long-term hypercapnia (1–5 kPa P co 2 , [17,95]). Melzner et al [3] observed a concentration-dependent effect of CO 2 on Na + K + -ATPase activity in cod gills with unchanged activity at 0.3 kPa P co 2 , and increased activity at 0.6 kPa P co 2 .…”

Section: Resultsmentioning

confidence: 99%

Impact of Ocean Acidification on Energy Metabolism of Oyster, Crassostrea gigas—Changes in Metabolic Pathways and Thermal Response

et al. 2010

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Climate change with increasing temperature and ocean acidification (OA) poses risks for marine ecosystems. According to Pörtner and Farrell [1], synergistic effects of elevated temperature and CO2-induced OA on energy metabolism will narrow the thermal tolerance window of marine ectothermal animals. To test this hypothesis, we investigated the effect of an acute temperature rise on energy metabolism of the oyster, Crassostrea gigas chronically exposed to elevated CO2 levels (partial pressure of CO2 in the seawater ~0.15 kPa, seawater pH ~ 7.7). Within one month of incubation at elevated Pco2 and 15 °C hemolymph pH fell (pHe = 7.1 ± 0.2 (CO2-group) vs. 7.6 ± 0.1 (control)) and Peco2 values in hemolymph increased (0.5 ± 0.2 kPa (CO2-group) vs. 0.2 ± 0.04 kPa (control)). Slightly but significantly elevated bicarbonate concentrations in the hemolymph of CO2-incubated oysters ([HCO− 3]e = 1.8 ± 0.3 mM (CO2-group) vs. 1.3 ± 0.1 mM (control)) indicate only minimal regulation of extracellular acid-base status. At the acclimation temperature of 15 °C the OA-induced decrease in pHe did not lead to metabolic depression in oysters as standard metabolism rates (SMR) of CO2-exposed oysters were similar to controls. Upon acute warming SMR rose in both groups, but displayed a stronger increase in the CO2-incubated group. Investigation in isolated gill cells revealed a similar temperaturedependence of respiration between groups. Furthermore, the fraction of cellular energy demand for ion regulation via Na+/K+-ATPase was not affected by chronic hypercapnia or temperature. Metabolic profiling using 1H-NMR spectroscopy revealed substantial changes in some tissues following OA exposure at 15 °C. In mantle tissue alanine and ATP levels decreased significantly whereas an increase in succinate levels was observed in gill tissue. These findings suggest shifts in metabolic pathways following OA-exposure. Our study confirms that OA affects energy metabolism in oysters and suggests that climate change may affect populations of sessile coastal invertebrates such as mollusks.

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Vacuolar-Type H⁺-ATPase and Na⁺, K⁺-ATPase Expression in Gills of Atlantic Salmon (Salmo salar) during Isolated and Combined Exposure to Hyperoxia and Hypercapnia in Fresh Water

Cited by 24 publications

References 30 publications

Changes in gene expression in gills of the euryhaline killifishFundulus heteroclitusafter abrupt salinity transfer

Changes in gene expression in gills of the euryhaline killifishFundulus heteroclitusafter abrupt salinity transfer

Energy homeostasis as an integrative tool for assessing limits of environmental stress tolerance in aquatic invertebrates

Impact of Ocean Acidification on Energy Metabolism of Oyster, Crassostrea gigas—Changes in Metabolic Pathways and Thermal Response

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