Vacuolar Transport of Secondary Metabolites and Xenobiotics

Martinoia, Enrico; Klein, Markus; Geisler, Markus; Sánchez-Fernández, Rociío; Rea, Philip A.

doi:10.1002/9781119312994.apr0044

Cited by 5 publications

(5 citation statements)

References 104 publications

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“…In addition, glycosylation is stimulated by water stress: initially, a high concentration of volatiles is synthetized as a defense mechanism used by the plant against the stress, and then glycosylation occurs because these high concentrations may be toxic to the plant itself . Several studies support the hypothesis that plants may produce glycosylate volatile compounds as a detoxification strategy because glycosides have been identified in the vacuoles of cells . Other studies also showed this effect on the biosynthesis of monoterpenes during dehydration.…”

Section: Resultsmentioning

confidence: 69%

“…37 Several studies support the hypothesis that plants may produce glycosylate volatile compounds as a detoxification strategy because glycosides have been identified in the vacuoles of cells. 38,39 Other studies also showed this effect on the biosynthesis of monoterpenes during dehydration. Off-vine dehydration under an ozone atmosphere (combined water and oxidative stress) increased the contents of terpenes, which are the major aromatic markers of Moscato bianco grapes, in the last phases of dehydration.…”

Section: Free Volatile Secondary Metabolitesmentioning

confidence: 87%

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On‐vine withering process of ‘Moscato bianco’ grapes: effect of cane‐cut system on volatile composition

et al. 2018

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A cane-cut, on-vine withering system applied at harvest induced changes in the volatile composition of Moscato bianco grapes increasing total volatile content, consisting mainly of bound compounds, by the 24th day of dehydration. The grapes dehydrated on-vine using this new system also showed significantly greater content of most of the free volatile compounds detected. © 2018 Society of Chemical Industry.

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Section: Resultsmentioning

confidence: 69%

Section: Free Volatile Secondary Metabolitesmentioning

confidence: 87%

On‐vine withering process of ‘Moscato bianco’ grapes: effect of cane‐cut system on volatile composition

et al. 2018

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“…The vacuolar membrane contains both a V‐type H + ‐translocating adenosine triphosphatase (H + ‐ATPase) and an H + ‐translocating pyrophosphatase (H + ‐PPase; Rea and Sanders ) and, depending on the energy source available, these pumps transport H + from the cytoplasmic side of the membrane into the lumen of the vacuole forming both a pH gradient and a membrane potential. This H + ‐electrochemical potential difference can then be used to drive transport across the membrane through an H + ‐antiporter (Martinoia et al ). MATE transporters have been identified as the H + ‐antiporters involved in the vacuolar transport of several flavonoid glucosides (Marinova et al , Zhao and Dixon , Zhao et al ).…”

Section: Introductionmentioning

confidence: 99%

Mechanistic differences in the uptake of salicylic acid glucose conjugates by vacuolar membrane‐enriched vesicles isolated from Arabidopsis thaliana

Vaca

Behrens

Theccanat

et al. 2017

Physiologia Plantarum

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Salicylic acid (SA) is a plant hormone involved in a number of physiological responses including both local and systemic resistance of plants to pathogens. In Arabidopsis, SA is glucosylated to form either SA 2-O-β-d-glucose (SAG) or SA glucose ester (SGE). In this study, we show that SAG accumulates in the vacuole of Arabidopsis, while the majority of SGE was located outside the vacuole. The uptake of SAG by vacuolar membrane-enriched vesicles isolated from Arabidopsis was stimulated by the addition of MgATP and was inhibited by both vanadate (ABC transporter inhibitor) and bafilomycin A (vacuolar H -ATPase inhibitor), suggesting that SAG uptake involves both an ABC transporter and H -antiporter. Despite its absence in the vacuole, we observed the MgATP-dependent uptake of SGE by Arabidopsis vacuolar membrane-enriched vesicles. SGE uptake was not inhibited by vanadate but was inhibited by bafilomycin A and gramicidin D providing evidence that uptake was dependent on an H -antiporter. The uptake of both SAG and SGE was also inhibited by quercetin and verapamil (two known inhibitors of multidrug efflux pumps) and salicin and arbutin. MgATP-dependent SAG and SGE uptake exhibited Michaelis-Menten-type saturation kinetics. The vacuolar enriched-membrane vesicles had a 46-fold greater affinity and a 10-fold greater transport activity with SGE than with SAG. We propose that in Arabidopsis, SAG is transported into the vacuole to serve as a long-term storage form of SA while SGE, although also transported into the vacuole, is easily hydrolyzed to release the active hormone which can then be remobilized to other cellular locations.

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“…La vacuol a ha sido descrita como un componente celular que ocupa cerca de 80% del volumen de la célula, aunque también puede haber vacuolas de menor tamaño, las cuales pueden distinguirse por el contenido de proteínas solubles y por su proteína de membrana (acuaporinas) (Martinoia et al, 2000). Las vacuolas sirven como reservorio de metabolitos y nutrientes, y participan en el proceso de regulación homeostática del citosol (Martinoia et al, 2000). La importancia de la vacuola en la tolerancia a metales ha sido demostrada en diversos trabajos (ver revisión de De, 2000).…”

Section: Quelación De Los Metales En El Citosol Por Diversos Ligandosunclassified

Mecanismos de tolerancia a elementos potencialmente tóxicos en plantas

Mendoza¹,

Pérez

2008

Bot. Sci.

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Plants possess a wide array of potential cellular mechanisms that may be involved in the tolerance to potentially toxic elements. These mechanisms include mycorrhizal associations, heavy metals binding to cell wall, precipitation by extracellular exudates; reduction in uptake or efflux pumping of metals at the plasma membrane, chelation of metals in the cytosol by peptides such as phytochelatins, metallothionein, histidina free, proline free , and the compartmentation of metals in the vacuole by tono-plast- located transporters. This review provides a broad overview of the evidence of the involvement of each mechanism in plants' tolerance to potentially toxic metals.

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Vacuolar Transport of Secondary Metabolites and Xenobiotics

Abstract: The sections in this article areIntroductionCommon Pathways for DetoxificationSecondary Energized Transporters andABCTransportersCharacteristics of VacuolarABCTransportersConclusions and OutlookNoteAcknowledgments

Cited by 5 publications

References 104 publications

On‐vine withering process of ‘Moscato bianco’ grapes: effect of cane‐cut system on volatile composition

On‐vine withering process of ‘Moscato bianco’ grapes: effect of cane‐cut system on volatile composition

Mechanistic differences in the uptake of salicylic acid glucose conjugates by vacuolar membrane‐enriched vesicles isolated from Arabidopsis thaliana

Mecanismos de tolerancia a elementos potencialmente tóxicos en plantas

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