Vacuolar Sorting Behaviors of 11S Globulins in Plant Cells

Mori, Takashi; Saruta, Yohei; Fukuda, Tomoko; Prak, Krisna; Ishimoto, Masao; Maruyama, Nobuyuki; Utsumi, Shigeru

doi:10.1271/bbb.80460

Cited by 8 publications

(5 citation statements)

References 36 publications

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“…Soybean protein storage vacuoles (PSVs) are temporal extension organelles of the ER, which specialise in accumulating and compartmentalising seed storage proteins (Kim and Krishnan 2004;Robinson et al 2005;Mori et al 2009). This intrinsic characteristic results in an absence or low density of aminopeptidases in the lumen, an internally neutral pH and a low protein degradation rate, all of which are condition that allow for long-term storage of immunogenic and fully active recombinant proteins (Takaiwa et al 2007).…”

Section: Introductionmentioning

confidence: 99%

Expression of functional recombinant human growth hormone in transgenic soybean seeds

et al. 2010

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We produced human growth hormone (hGH), a protein that stimulates growth and cell reproduction, in genetically engineered soybean [Glycine max (L.) Merrill] seeds. Utilising the alpha prime (α') subunit of β-conglycinin tissue-specific promoter from soybean and the α-Coixin signal peptide from Coix lacryma-jobi, we obtained transgenic soybean lines that expressed the mature form of hGH in their seeds. Expression levels of bioactive hGH up to 2.9% of the total soluble seed protein content (corresponding to approximately 9 g kg(-1)) were measured in mature dry soybean seeds. The results of ultrastructural immunocytochemistry assays indicated that the recombinant hGH in seed cotyledonary cells was efficiently directed to protein storage vacuoles. Specific bioassays demonstrated that the hGH expressed in the soybean seeds was fully active. The recombinant hGH protein sequence was confirmed by mass spectrometry characterisation. These results demonstrate that the utilisation of tissue-specific regulatory sequences is an attractive and viable option for achieving high-yield production of recombinant proteins in stable transgenic soybean seeds.

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Section: Introductionmentioning

confidence: 99%

Expression of functional recombinant human growth hormone in transgenic soybean seeds

et al. 2010

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“…However, ingredients such as B. monnieri , Citicoline and L‐Theanine have been found to benefit IR and working memory (Calabrese et al., 2008; Pase et al., 2011; Sadhu et al., 2014; Stough et al., 2008). Other ingredients such as Lion's Mane Mushroom and Phosphatidylserine have been shown to generally benefit memory (Crook et al., 1991; Mori et al., 2009). These ingredients are commonly found in other nootropics such as Alpha Brain, Modafinil, Noocube and Qualia Mind and these products have also been associated with improvements in memory (Battleday & Brem, 2015; Snow et al., 2021; Solomon et al., 2016; Stough et al., 2015).…”

Section: Discussionmentioning

confidence: 99%

Efficacy of the nootropic supplement Mind Lab Pro on memory in adults: Double blind, placebo‐controlled study

Abbott‐Imboden,

Gonzalez,

Utley

2023

Human Psychopharmacology

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Objectives This study aimed to investigate the efficacy of taking Mind Lab Pro, a plant‐based nootropic on memory in a group of healthy adults. Auditory, visual, visual working memory, immediate and delayed recall (DR) were assessed. Methods The study employed a pseudo randomised, double blinded, placebo‐controlled design. A total of 49 healthy individuals completed the study with 36 in the experimental group and 13 in the control group. Participants ranged between 20 and 68 years with a mean age of 31.4 ± 14.4 years. Pre and post taking either the Mind Lab Pro supplement or placebo for 30 days. All participants completed the Wechsler Memory Scale Fourth UK Edition (WSM‐IV UK). Results We found that the experimental group significantly improved in all memory subtests assessed (p < 0.05) whilst the control group only significantly improved in auditory memory and immediate recall (p = 0.004 and p = 0.014 respectively). A significant difference in immediate and DR was also found between the control and experimental group (p = 0.005 and 0.034 respectively). Conclusion The use of Mind Lab Pro for 4 weeks improves memory with the experimental group significantly improving in all sub areas of memory as assessed by the WSM‐IV UK.

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“…11S globulins from pea ( P. sativum ), pumpkin and soybean (two types) have been expressed in BY-2 cells (Mori et al ., 2009). Trimeric pro-forms of the three globulin types were detected, although the expression level of the pumpkin 11S globulin was very low, and one soybean globulin appeared not to be expressed at all.…”

Section: Discussionmentioning

confidence: 99%

Successful transport to the vacuole of heterologously expressed mung bean 8S globulin occurs in seed but not in vegetative tissues

Wang

Shen

Cai

et al. 2013

Journal of Experimental Botany

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This study investigated the subcellular location of mung bean (Vigna radiata) 8S globulin in transient expression systems as well as in tobacco (Nicotiana tabacum) BY-2 cells and different tissues from a transgenic Arabidopsis (Arabidopsis thaliana) line stably expressing this storage globulin. When transiently expressed in protoplasts from both BY-2 cells and Arabidopsis suspension cultured cells, the 8S globulin located to structures that were neither Golgi nor pre-vacuolar compartments (PVCs). Immunogold electron microscopy of the transgenics reveals the 8S globulin-positive structures to be small, spherical, ribosome-covered endoplasmic reticulum (ER)-derived bodies. In BY-2 cells and all vegetative cells, the 8S globulin was present as a pro-form. However, in Arabidopsis embryos, with the onset of endogenous storage protein synthesis, the 8S globulin exited the ER and passed through the PVC to the protein storage vacuole where it was processed to its smaller mature form. These results clearly demonstrated that, when taken out of context and expressed in vegetative cells, the mung bean 8S storage globulin cannot exit the ER, and indicate that natural targeting of storage proteins to the vacuole should be better studied in the maturing seed.

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Vacuolar Sorting Behaviors of 11S Globulins in Plant Cells

Cited by 8 publications

References 36 publications

Expression of functional recombinant human growth hormone in transgenic soybean seeds

Expression of functional recombinant human growth hormone in transgenic soybean seeds

Efficacy of the nootropic supplement Mind Lab Pro on memory in adults: Double blind, placebo‐controlled study

Successful transport to the vacuole of heterologously expressed mung bean 8S globulin occurs in seed but not in vegetative tissues

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