Vaccum aspiratory puncture system for Leishmania culturing, isolation and transport: preliminary report

Sensitivity of a Vacuum Aspiratory Culture Technique for Diagnosis of Localized Cutaneous Leishmaniasis in an Endemic Area of Leishmania (Viannia) braziliensis Transmission

“…Culture of three aspirates significantly improved sensitivity. The contamination rate in our study was very low (2.9%) compared to 28.6% in the original proposal (Marzochi et al 1993). The greater sensiivity could be explained by modifications of culture medium specially those of lowvolume liquid phase which was not enriched as previously recommended by Shaw and Lainson (1981).…”

“…has shown variable results depending on quality of culture media (Shaw & Lainson 1981, Cuba et al 1986), collection technique (Evans 1989) and other characteristics such as disease duration and previous use of anti-leishmanial drugs (Cuba et al 1984). Marzochi et al (1993) described a new collection technique using direct inoculation of culture tubes containing NNN media with a liquid phase through a skin puncture system with five to ten mililiters vacuum.…”

“…has shown variable results depending on quality of culture media (Shaw & Lainson 1981, Cuba et al 1986), collection technique (Evans 1989) and other characteristics such as disease duration and previous use of anti-leishmanial drugs (Cuba et al 1984). Marzochi et al (1993) described a new collection technique using direct inoculation of culture tubes containing NNN media with a liquid phase through a skin puncture system with five to ten mililiters vacuum.We modified the culture medium using a smaller amount of an unenriched liquid phase composed of isotonic saline solution with gentamicin, resuming 15 ml vacuum in each tube. We used the same aspiratory puncture system with commercial needles but the aspiration was modified by allowing air to come into the tubes when pulling the needle out to obtain a larger amount of inoculum.…”

Aspectos epidemiológicos da leishmaniose tegumentar americana em cães, no Município de Paraty, Estado do Rio de Janeiro, Brasil

“…Concomitantemente à biópsia, foi realizada a técnica de punção aspirativa a vácuo proposta por Marzochi et al (1993). Os isolamentos foram realizados no Laboratório de Protozoologia do Departamento de Ciências Bioló-gicas, Escola Nacional de Saúde Pública, Fiocruz.…”

Sensitivity of Lymph Node Aspiration in Localized Cutaneous Leishmaniasis Due to Leishmania (Viannia) braziliensis

“…We performed aspiration cultures from skin and enlarged lymph nodes in patients with L.V.b. in- fections using a modified technique following the original principles suggested by Marzochi et al (1993).…”