Vaccinia virus host range genes

Perkus, Marion E.; Goebel, Scott J.; Davis, Stephen W.; Johnson, Gerard P.; Limbach, Keith; Norton, Elizabeth; Paoletti, Enzo

doi:10.1016/0042-6822(90)90296-4

Cited by 175 publications

(169 citation statements)

References 31 publications

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“…This interaction had been previously described, as well as that between E3L and the substrate binding domain of the host IFN-induced doublestranded RNA-activated protein kinase (53,54). An interaction between the K1L protein, required for replication in rabbit kidney and human cell lines (55), with the uncharacterized C10L protein was recently found by using another version of the yeast two-hybrid system (A. Grunhaus and B.M., unpublished data) and was confirmed in the present screening. The biological relationship between the K1L and C1OL proteins remains to be determined.…”

Section: Virion Structural͞morphogenesis Proteinsmentioning

confidence: 69%

Genome-wide analysis of vaccinia virus protein–protein interactions

McCraith

Holtzman²,

Moss³

et al. 2000

Proc. Natl. Acad. Sci. U.S.A.

265

205

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To detect interactions between proteins of vaccinia virus, we carried out a comprehensive two-hybrid analysis to assay every pairwise combination. We constructed an array of yeast transformants that contained each of the 266 predicted viral ORFs as Gal4 activation domain hybrid proteins. The array was individually mated to transformants containing each ORF as a Gal4 -DNAbinding domain hybrid, and diploids expressing the two-hybrid reporter gene were identified. Of the Ϸ70,000 combinations, we found 37 protein-protein interactions, including 28 that were previously unknown. In some cases, e.g., late transcription factors, both proteins were known to have related roles although there was no prior evidence of physical associations. For some other interactions, neither protein had a known role. In the majority of cases, however, one of the interacting proteins was known to be involved in DNA replication, transcription, virion structure, or host evasion, thereby providing a clue to the role of the other uncharacterized protein in a specific process.

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Section: Virion Structural͞morphogenesis Proteinsmentioning

confidence: 69%

Genome-wide analysis of vaccinia virus protein–protein interactions

McCraith

Holtzman²,

Moss³

et al. 2000

Proc. Natl. Acad. Sci. U.S.A.

265

205

View full text Add to dashboard Cite

show abstract

“…The expression plasmid pMK4 was used in vivo to generate a recombinant virus (vP 1258) by using a New York Vaccinia (NYVAC) rescuing virus (14). The COPAK donor plasmid contains multiple cloning sites, with the gene of interest being placed under the control of an early-late H6 promoter (15). The plasmid also carries the K1L ORF flanked on either side with ORFs of the A24R and A27L (16).…”

Section: Methodsmentioning

confidence: 99%

Boosting with recombinant vaccinia increases immunogenicity and protective efficacy of malaria DNA vaccine

Sedegah

Jones

Kaur

et al. 1998

Proc. Natl. Acad. Sci. U.S.A.

207

150

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“…This suggested that another gene functionally equivalent to K1L for multiplication in human cells was present in the VV genome. Indeed, Perkus et aL (1990) discovered that the C7L ORF on the HindlII restriction map of VV displays the properties predicted for a functional homologue of the K1L ORF. In an independent search for a VV host range gene with similar properties to K1L we have confirmed the host range function of the C7L ORF.…”

mentioning

confidence: 99%

Detection of a protein encoded by the vaccinia virus C7L open reading frame and study of its effect on virus multiplication in different cell lines

Oguiura

Spehner

Drillien

1993

Journal of General Virology

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Vaccinia virus encodes several proteins, the activity of which is essential for multiplication in different cell types. Both the C7L and K1L open reading frames (ORFs) have been characterized as viral determinants for multiplication in human cells. To confirm and extend these findings we inserted the C7L ORF into the genome of a mutant virus unable to multiply in human cells and showed that this virus recovered its ability to replicate. Deletion of C7L from a wild-type viral genome did not adversely affect virus multiplication in human cells but it did reduce replication in hamster Dede ceils. When both C7L and K1L were deleted from the vaccinia virus genome only poor or no viral yields were obtained from various human cell lines. Recombinant viruses were also constructed to facilitate the study of C7L protein synthesis during infection. One virus in which the lacZ ORF was fused downstream and in-frame with the C7L ORF enabled us to characterize the C7L protein as an early gene product. Another recombinant virus was constructed so that the carboxy terminus of the C7L ORF product contained an additional 28 amino acids from the carboxy terminus of K1L. Tagging of C7L in this way allowed us to detect the fusion protein by immunoprecipitation with antibodies against the K1L protein. Furthermore, the hybrid protein retained its biological properties. The recombinant viruses constructed in this work should be useful for studies of the molecular basis of the activity of viral host range proteins.

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Vaccinia virus host range genes

Cited by 175 publications

References 31 publications

Genome-wide analysis of vaccinia virus protein–protein interactions

Genome-wide analysis of vaccinia virus protein–protein interactions

Boosting with recombinant vaccinia increases immunogenicity and protective efficacy of malaria DNA vaccine

Detection of a protein encoded by the vaccinia virus C7L open reading frame and study of its effect on virus multiplication in different cell lines

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