Vaccines against influenza A viruses in poultry and swine: Status and future developments

Rahn, Jessica; Hoffmann, Donata; Harder, Timm C.; Beer, Martin

doi:10.1016/j.vaccine.2015.03.052

Cited by 39 publications

(29 citation statements)

References 218 publications

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“…Furthermore, the Bgag as a vaccine component can be a useful marker if the need arises to confirm the fact of vaccination. This can be especially important for veterinary applications, in which differentiation of infected from vaccinated animals (DIVA) is important (Rahn et al, 2015; Suarez, 2012). …”

Section: Discussionmentioning

confidence: 99%

Preparation of quadri-subtype influenza virus-like particles using bovine immunodeficiency virus gag protein

et al. 2016

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Influenza VLPs comprised of hemagglutinin (HA), neuraminidase (NA), and matrix (M1) proteins have been previously used for immunological and virological studies. Here we demonstrated that influenza VLPs can be made in Sf9 cells by using the bovine immunodeficiency virus gag (Bgag) protein in place of M1. We showed that Bgag can be used to prepare VLPs for several influenza subtypes including H1N1 and H10N8. Furthermore, by using Bgag, we prepared quadri-subtype VLPs, which co-expressed within the VLP the four HA subtypes derived from avian-origin H5N1, H7N9, H9N2 and H10N8 viruses. VLPs showed hemagglutination and neuraminidase activities and reacted with specific antisera. The content and co-localization of each HA subtype within the quadri-subtype VLP were evaluated. Electron microscopy showed that Bgag-based VLPs resembled influenza virions with the diameter of 150-200 nm. This is the first report of quadri-subtype design for influenza VLP and the use of Bgag for influenza VLP preparation.

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Section: Discussionmentioning

confidence: 99%

Preparation of quadri-subtype influenza virus-like particles using bovine immunodeficiency virus gag protein

et al. 2016

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“…The role of cell-mediated immunity remains to be studied. The presence of BIV gag in the VLPs can be important when differentiation of infected from vaccinated animals (DIVA) is important [26, 27]. DIVA is needed to allow serologic surveillance to be used to determine if field virus is still circulating in poultry flocks which have been vaccinated.…”

Section: Discussionmentioning

confidence: 99%

Vaccination with virus-like particles containing H5 antigens from three H5N1 clades protects chickens from H5N1 and H5N8 influenza viruses

Kapczynski

Tumpey

Hidajat

et al. 2016

Vaccine

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Highly pathogenic avian influenza (HPAI) viruses, especially H5N1 strains, represent a public health threat and cause widespread morbidity and mortality in domestic poultry. Recombinant virus-like particles (VLPs) represent a promising novel vaccine approach to control avian influenza including HPAI strains. Influenza VLPs contain viral hemagglutinin (HA), which can be expressed in cell culture within highly immunogenic VLPs that morphologically and antigenically resemble influenza virions, except VLPs are non-infectious. Here we describe a recombinant VLP containing HA proteins derived from three distinct clades of H5N1 viruses as an experimental, broadly protective H5 avian influenza vaccine. A baculovirus vector was configured to co-express the H5 genes from recent H5N1 HPAI isolates A/chicken/Germany/2014 (clade 2.3.4.4), A/chicken/West Java/Subang/29/2007 (clade 2.1.3) and A/chicken/Egypt/121/2012 (clade 2.2.1). Co-expression of these genes in Sf9 cells along with influenza neuraminidase (NA) and retrovirus gag genes resulted in production of triple-clade H555 VLPs that exhibited hemagglutination activity and morphologically resembled influenza virions. Vaccination of chickens with these VLPs resulted in induction of serum antibody responses and efficient protection against experimental challenges with three different viruses including the recent U.S. H5N8 HPAI isolate. We conclude that these novel triple-clade VLPs represent a feasible strategy for simultaneously evoking protective antibodies against multiple variants of H5 influenza virus.

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“…Poultry accounts for almost half of all meat consumed in the UK, with 875 million chickens, 17 million turkeys, 16 million ducks and 250,000 geese a year supplied by over 2500 poultry farms [1]. Their production can be adversely affected by infection with avian specific viruses such as infectious bursal disease virus (IBDV), infectious bronchitis virus (IBV) and Newcastle virus (NDV) [2][3][4][5][6][7]. Poultry can also serve as the source of zoonotic, or potentially zoonotic, infections with viruses such as H5N1 and H7N9, transmitted to humans through contact with poultry.…”

Section: Introductionmentioning

confidence: 99%

Accurate characterization of the IFITM locus using MiSeq and PacBio sequencing shows genetic variation in Galliformes

et al. 2017

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BackgroundInterferon inducible transmembrane (IFITM) proteins are effectors of the immune system widely characterized for their role in restricting infection by diverse enveloped and non-enveloped viruses. The chicken IFITM (chIFITM) genes are clustered on chromosome 5 and to date four genes have been annotated, namely chIFITM1, chIFITM3, chIFITM5 and chIFITM10. However, due to poor assembly of this locus in the Gallus Gallus v4 genome, accurate characterization has so far proven problematic. Recently, a new chicken reference genome assembly Gallus Gallus v5 was generated using Sanger, 454, Illumina and PacBio sequencing technologies identifying considerable differences in the chIFITM locus over the previous genome releases.MethodsWe re-sequenced the locus using both Illumina MiSeq and PacBio RS II sequencing technologies and we mapped RNA-seq data from the European Nucleotide Archive (ENA) to this finalized chIFITM locus. Using SureSelect probes capture probes designed to the finalized chIFITM locus, we sequenced the locus of a different chicken breed, namely a White Leghorn, and a turkey.ResultsWe confirmed the Gallus Gallus v5 consensus except for two insertions of 5 and 1 base pair within the chIFITM3 and B4GALNT4 genes, respectively, and a single base pair deletion within the B4GALNT4 gene. The pull down revealed a single amino acid substitution of A63V in the CIL domain of IFITM2 compared to Red Jungle fowl and 13, 13 and 11 differences between IFITM1, 2 and 3 of chickens and turkeys, respectively. RNA-seq shows chIFITM2 and chIFITM3 expression in numerous tissue types of different chicken breeds and avian cell lines, while the expression of the putative chIFITM1 is limited to the testis, caecum and ileum tissues.ConclusionsLocus resequencing using these capture probes and RNA-seq based expression analysis will allow the further characterization of genetic diversity within Galliformes.Electronic supplementary materialThe online version of this article (doi:10.1186/s12864-017-3801-8) contains supplementary material, which is available to authorized users.

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Vaccines against influenza A viruses in poultry and swine: Status and future developments

Cited by 39 publications

References 218 publications

Preparation of quadri-subtype influenza virus-like particles using bovine immunodeficiency virus gag protein

Preparation of quadri-subtype influenza virus-like particles using bovine immunodeficiency virus gag protein

Vaccination with virus-like particles containing H5 antigens from three H5N1 clades protects chickens from H5N1 and H5N8 influenza viruses

Accurate characterization of the IFITM locus using MiSeq and PacBio sequencing shows genetic variation in Galliformes

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