Vaccine potential of a tick vitellin-degrading enzyme (VTDCE)

Seixas, Adriana; Leal, Alexandre Trindade; Nascimento-Silva, Maria Clara L.; Masuda, Aoi; Termignoni, Carlos; Vaz, Itabajara da Silva

doi:10.1016/j.vetimm.2008.04.001

Cited by 48 publications

(36 citation statements)

References 39 publications

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“…In previous studies, we evaluated the efficacy of candidate anti-tick vaccines from H. longicornis, R. appendiculatus (Imamura et al , 2006Mulenga et al 1999;Sugino et al 2003;Tsuda et al 2001) and R. microplus (Leal et al 2006;Seixas et al 2008). The results demonstrated that vaccination efficacy in the host is expressed as an increase in feeding duration, a decrease in weight of engorged ticks, as inhibition of molting, death of feeding ticks, impaired egg production (Imamura et al , 2006Mulenga et al 1999;Tsuda et al 2001;Sugino et al 2003), and as the inhibition of pathogen transmission via tick feeding (Imamura et al 2008).…”

Section: Discussionmentioning

confidence: 99%

“…Many studies have observed an increase in mortality rate in female ticks and/or a decrease in egg laying capacity after tick-protein vaccinations (Imamura et al , 2008Seixas et al 2008). Anti-tick immunity induced by the rHLMP1-immunization impaired the tick, as evidenced by reduction in the number and increase in the mortality rates of the ticks fed on rHLMP1-immunized rabbits.…”

Section: Discussionmentioning

confidence: 99%

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Effect of vaccination with a recombinant metalloprotease from Haemaphysalis longicornis

et al. 2009

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We report the cloning, expression and characterization of an Haemaphysalis longicornis metalloprotease (named HLMP1). The gene encodes a predicted 550 aminoacid protein with similarity to metalloproteases of the reprolysin family. The protein sequence contains a signal sequence, the zinc-binding motif (HEXXHXXGXXH) common to metalloproteases and a cysteine-rich region. Reverse transcription-PCR expression analysis indicates the presence of mRNA in the salivary gland of larva, nymph and adult ticks. Rabbit repeatedly infested with H. longicornis recognized rHLMP1, suggesting that the immune-response against HLMP1 is naturally induced through the feeding of ticks. Vaccination of rabbit with rHLMP1 produced protective immunity against ticks, resulting in 15.6 and 14.6% mortality in nymph and adult ticks, respectively. This work provides information to understand the tick's defense system, and offers new insights to develop strategies to block this defense system with an anti-tick vaccine based on a metalloprotease.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Effect of vaccination with a recombinant metalloprotease from Haemaphysalis longicornis

et al. 2009

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“…These compounds include vitellogenin, which is the precursor for the yolk protein vitellin, an essential nutrient during early embryogenesis (Seixas et al, 2012). A range of proteases involved with the hydrolysis of vitellin, leading to yolk degradation, have been isolated in eggs of the cattle tick R. microplus Sorgine et al, 2000;Seixas et al, 2008) and targeted via vaccination. This has led to reduction in tick fecundity and next generation egg weight in ticks fed on the blood of vaccinated bovine hosts Seixas et al, 2008).…”

Section: Alternative and Novel Targetsmentioning

confidence: 99%

“…A range of proteases involved with the hydrolysis of vitellin, leading to yolk degradation, have been isolated in eggs of the cattle tick R. microplus Sorgine et al, 2000;Seixas et al, 2008) and targeted via vaccination. This has led to reduction in tick fecundity and next generation egg weight in ticks fed on the blood of vaccinated bovine hosts Seixas et al, 2008). Of these proteases vitellin-degrading cysteine endopeptidase, a Cathepsin L-like protein, is the most active enzyme.…”

Section: Alternative and Novel Targetsmentioning

confidence: 99%

Understanding the biology and control of the poultry red miteDermanyssus gallinae: a review

et al. 2015

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Dermanyssus gallinae, the poultry red mite (PRM), is a blood-feeding ectoparasite capable of causing pathology in birds, amongst other animals. It is an increasingly important pathogen in egg layers and is responsible for substantial economic losses to the poultry industry worldwide. Even though PRM poses a serious problem, very little is known about the basic biology of the mite. Here we review the current body of literature describing red mite biology and discuss how this has been, or could be, used to develop methods to control PRM infestations. We focus primarily on the PRM digestive system, salivary glands, nervous system and exoskeleton and also explore areas of PRM biology which have to date received little or no study but have the potential to offer new control targets.

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“…Since BYC immunization interferes in functions related to the target protein, in this case those related to embryonic and egg development, it is possible to assume that vaccination with other embryo proteins can be efficient to intervene in tick embryonic and larval phases. Recently, it has been shown that immunization of bovines with a cysteine endopeptidase -VTDCE, an enzyme also involved in the digestion of vitellin (SEIXAS et al, 2003), leads to a certain degree of immunoprotection (21% of overall protection rate) in vaccinated cattle (SEIXAS et al, 2008).…”

Section: Boophilus Yolk Pro-cathepsin and Tick Vitellin-degrading Enzymementioning

confidence: 99%

New approaches toward anti-Rhipicephalus (Boophilus) microplus tick vaccine

Parizi¹,

Pohl²,

Masuda³

et al. 2009

RBPV

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The tick Rhipicephalus (Boophilus) microplus (formerly Boophilus microplus) is the major ectoparasite affecting livestock in America, Asia, Africa, and Oceania. Conventional tick control is based on the use of acaricides but immunization of bovines with tick gut proteins induces only a partial protective immune response. Based on this information, distinct research groups have explored the possibility of protecting the animals by inducing an immune response against other tick proteins. However, the antigens so far described do not induce the necessary protection for suppressing the use of acaricides. Currently, several groups are engaged in identifying new tick proteins to be used as targets for the development of new vaccines. This approach focuses on the enhancement of the immunogenicity of antigens already tested by incorporating new adjuvants or formulations and by searching for new antigens. This paper reviews the work done by Brazilian researchers to develop a vaccine against this tick.Keywords: Boophilus microplus, Rhipicephalus microplus, vaccine, tick, control. ResumoO carrapato Rhipicephalus (Boophilus) microplus (anteriormente Boophilus microplus) é o principal ectoparasita que afeta bovinos na América, Ásia, África e Oceania e o seu controle é tradicionalmente realizado através do uso de acaricidas. Experimentos de imunização com proteínas do carrapato mostram que a resposta imune desenvolvida pelos bovinos vacinados protege, em parte, os animais do parasitismo. Baseado nessas observações, vários grupos de pesquisa exploram a possibilidade de proteger os animais pela indução de uma resposta imune contra proteínas do carrapato. Entretanto, os antígenos já caracterizados não asseguram o grau de proteção necessário para suprimir o uso de acaricidas. Portanto, esses grupos de pesquisa estão engajados na tentativa de identificar novas proteínas que possam ser utilizadas para o desenvolvimento de novas vacinas, as quais possam induzir maior imunogenicidade de que os antígenos já testados, através do uso de novas formulações e/ou pela incorporação de adjuvantes. O presente artigo apresenta uma revisão da literatura sobre os resultados obtidos por pesquisadores brasileiros no desenvolvimento de vacinas contra o carrapato.

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Vaccine potential of a tick vitellin-degrading enzyme (VTDCE)

Cited by 48 publications

References 39 publications

Effect of vaccination with a recombinant metalloprotease from Haemaphysalis longicornis

Effect of vaccination with a recombinant metalloprotease from Haemaphysalis longicornis

Understanding the biology and control of the poultry red miteDermanyssus gallinae: a review

New approaches toward anti-Rhipicephalus (Boophilus) microplus tick vaccine

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