Vaccination with β₂‐Microglobulin‐Deficient Dendritic Cells Protects Against Growth of β₂‐Microglobulin‐Deficient Tumours

Abstract

Defects in cell surface expression of major histocompatibility complex class I antigen molecules are common in tumour cells. We have previously described the generation of adaptive immunity to tumour cells deficient in the transporter associated with antigen processing molecule. In this study, we demonstrate enhanced in vivo protection against growth of β2‐microglobulin‐deficient tumour cells in syngeneic C57Bl/6 mice, following vaccination with β2‐microglobulin‐deficient dendritic cells. In vitro analysis suggested that vaccinated mice produced CD3+ cells, which could induce apoptosis in syngeneic β2‐microglobulin‐deficient tumour and non‐malignant cells. Further investigation of target cell recognition suggested that also tumour cells lacking expression of classical major histocompatibility complex class I heavy chains and functional transporter associated with antigen processing molecules were recognized by CD3+ effector cells from vaccinated mice. Histopathological examination of organs from vaccinated mice showed no significant vaccination‐induced pathology. The present findings point to a new possible strategy to counteract the growth of major histocompatibility complex class I‐deficient tumour cells.

In-Vivo Antidiabetic Activity and Safety Profile of Fruits Extract and Fraction of Solanum Terminale Forsk (Solanaceae)

“…Animals were humanely sacrificed and the vital organs (kidney, lungs, heart, liver, spleen and intestines) isolated, weighed and examined for macroscopic changes and/or development of any lesions [11], then stored in 40% formalin. Features of both treated and control groups were compared and then histologically evaluated by the Department of Pathology -MUHAS.…”

“…Histological evaluation and photomicrography was performed by the Histopathologist using an Olympus (CX31RBSF Model) light microscope equipped with a digital camera (Olympus Corporation, Tokyo, Japan). Tissue toxicity (damage) was evaluated under the microscope on 7 lowpower fields (x10 magnification) as well as on their high-power fields (x40 magnification) while taking pictures [11,12]. Picture processing and printing was performed using Adobe Photoshop 7.0 (Adobe Systems Incorporated, San Jose, CA, USA).…”